{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:30:03Z","timestamp":1792715403293,"version":"4.5.0"},"publisher-location":"Totowa, NJ","reference-count":30,"publisher":"Humana Press","isbn-type":[{"value":"9781617796203","type":"print"},{"value":"9781617796210","type":"electronic"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012]]},"DOI":"10.1007\/978-1-61779-621-0_7","type":"book-chapter","created":{"date-parts":[[2012,4,3]],"date-time":"2012-04-03T17:50:43Z","timestamp":1333475443000},"page":"115-125","source":"Crossref","is-referenced-by-count":33,"title":["The Fluorescence Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors"],"prefix":"10.1007","author":[{"given":"Aeron C.","family":"Hurt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Okomo-Adhiambo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larisa V.","family":"Gubareva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,2,18]]},"reference":[{"key":"7_CR1_7","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1146\/annurev.med.59.061506.213121","volume":"59","author":"A Moscona","year":"2008","unstructured":"Moscona, A. (2008) Medical management of influenza infection. Annu. Rev. Med. 59, 397\u2013413.","journal-title":"Annu. Rev. Med."},{"key":"7_CR2_7","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/0166-3542(95)00914-0","volume":"29","author":"FG Hayden","year":"1996","unstructured":"Hayden, F. G. (1996) Combination antiviral therapy for respiratory virus infections. Antiviral Res. 29, 45\u201348.","journal-title":"Antiviral Res."},{"key":"7_CR3_7","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1001\/jama.295.8.joc60020","volume":"295","author":"RA Bright","year":"2006","unstructured":"Bright, R. A., Shay, D. K., Shu, B., Cox, N. J., and Klimov, A. I. (2006) Adamantane resistance among influenza A viruses isolated early during the 2005\u20132006 influenza season in the United States. JAMA 295, 891\u2013894.","journal-title":"JAMA"},{"key":"7_CR4_7","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1086\/518936","volume":"196","author":"VM Deyde","year":"2007","unstructured":"Deyde, V.M.; Xu; X.; Bright, R.A.; Shaw, M.; Smith, C.B.; Zhang, Y.; Shu, Y.; Gubareva, L.V.; Cox, N.J.; Klimov, A.I. (2007) Surveillance of resistance to adamantanes among influenza A(H3N2) and A(H1N1) viruses isolated worldwide. J. Infect. Dis., 196, 249\u2013257.","journal-title":"J. Infect. Dis."},{"key":"7_CR5_7","doi-asserted-by":"publisher","first-page":"2269","DOI":"10.3390\/v2102269","volume":"2","author":"M Okomo-Adhiambo","year":"2010","unstructured":"Okomo-Adhiambo, M., Sleeman, K., Ballenger, K., Nguyen, H.T., Mishin, V.P., Sheu, T.G., Smagala, J., Li, Y., Klimov, A.I., Gubareva, L.V. (2010) Neuraminidase Inhibitor Susceptibility Testing in Human Influenza Viruses: A Laboratory Surveillance Perspective. Viruses, 2, 2269\u20132289.","journal-title":"Viruses"},{"key":"7_CR6_7","unstructured":"CDC Website http:\/\/www.cdc.gov\/h1n1flu\/eua\/peramivir.htm (accessed on September 23, 2011)."},{"key":"7_CR7_7","unstructured":"Shionogi and Company Website http:\/\/www.shionogi.co.jp\/ir_en\/news\/detail\/e_100126.pdf (accessed on September 23, 2011)."},{"key":"7_CR8_7","doi-asserted-by":"crossref","unstructured":"Sugaya N, Ohashi Y: (2010) Long-Acting Neuraminidase Inhibitor Laninamivir Octanoate (CS-8958) versus Oseltamivir as Treatment for Children with Influenza Virus Infection. Antimicrob Agents Chemother. AAC.01755-09","DOI":"10.1128\/AAC.01755-09"},{"key":"7_CR9_7","doi-asserted-by":"crossref","first-page":"2972","DOI":"10.1128\/JVI.67.6.2972-2980.1993","volume":"67","author":"PM Colman","year":"1993","unstructured":"Colman, P.M., Hoyne, P.A., Lawrence, M.C. (1993) Sequence and structure alignment of paramyxovirus hemagglutinin-neuraminidase with influenza virus neuraminidase. J Virol., 67, 2972\u20132980.","journal-title":"J Virol."},{"key":"7_CR10_7","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.jcv.2007.10.020","volume":"41","author":"O Ferraris","year":"2008","unstructured":"Ferraris, O., Lina, B. (2008) Mutations of neuraminidase implicated in neuraminidase inhibitors resistance. J. Clin. Virol., 41, 13\u201319.","journal-title":"J. Clin. Virol."},{"key":"7_CR11_7","doi-asserted-by":"publisher","first-page":"2395","DOI":"10.1128\/AAC.01339-05","volume":"50","author":"AS Monto","year":"2006","unstructured":"Monto, A.S., McKimm-Breschkin, J.L., Macken, C., Hampson, A.W., Hay, A., Klimov, A., Tashiro, M., Webster, R.G., Aymard, M., Hayden, F.G., Zambon, M. (2006) Detection of influenza viruses resistant to neuraminidase inhibitors in global surveillance during the first 3 years of their use. Antimicrob. Agents Chemother., 50, 2395\u20132402.","journal-title":"Antimicrob. Agents Chemother."},{"key":"7_CR12_7","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.virusres.2004.02.033","volume":"103","author":"BA Mungall","year":"2004","unstructured":"Mungall, B.A., Xu, X., Klimov, A. (2004) Surveillance of influenza isolates for susceptibility to neuraminidase inhibitors during the 2000\u20132002 influenza seasons. Virus Res., 103, 195\u2013197.","journal-title":"Virus Res."},{"key":"7_CR13_7","doi-asserted-by":"publisher","first-page":"3284","DOI":"10.1128\/AAC.00555-08","volume":"52","author":"TG Sheu","year":"2008","unstructured":"Sheu, T.G., Deyde, V.M., Okomo-Adhiambo, M., Garten, R.J., Xu, X., Bright, R.A., Butler, E.N., Wallis, T.R., Klimov, A.I., Gubareva, L.V. (2008) Surveillance for neuraminidase inhibitor resistance among human influenza A and B viruses circulating worldwide from 2004 to 2008. Antimicrob. Agents Chemother., 52, 3284\u20133292.","journal-title":"Antimicrob. Agents Chemother."},{"key":"7_CR14_7","doi-asserted-by":"publisher","first-page":"1034","DOI":"10.1001\/jama.2009.294","volume":"301","author":"NJ Dharan","year":"2009","unstructured":"Dharan, N. J., Gubareva, L. V., Meyer, J. J., Okomo-Adhiambo, M., McClinton, R. C., Marshall, S. A., St, G. K., Epperson, S., Brammer, L., Klimov, A. I., Bresee, J. S., and Fry, A. M. (2009) Infections with oseltamivir-resistant influenza A(H1N1) virus in the United States. JAMA 301, 1034\u20131041.","journal-title":"JAMA"},{"key":"7_CR15_7","doi-asserted-by":"publisher","first-page":"155","DOI":"10.3201\/eid1502.081031","volume":"15","author":"SH Hauge","year":"2009","unstructured":"Hauge, S. H., Dudman, S., Borgen, K., Lackenby, A., and Hungnes, O. (2009). Oseltamivir-resistant influenza viruses A (H1N1), Norway, 2007\u201308. Emerg. Infect. Dis. 15, 155\u2013162.","journal-title":"Emerg. Infect. Dis."},{"key":"7_CR16_7","doi-asserted-by":"crossref","first-page":"552","DOI":"10.3201\/eid1504.181280","volume":"15","author":"A. Meijer","year":"2009","unstructured":"Meijer, A., Lackenby, A., Hungnes, O., Lina, B., van-der-Werf, S., Schweiger, B., Opp, M., Paget, J., van-de-Kassteele, J., Hay, A., and Zambon, M. (2009) Oseltamivir-resistant influenza virus A (H1N1), Europe, 2007\u201308 Season. Emerg. Infect. Dis. 15, 552\u2013560.","journal-title":"Emerg. Infect. Dis."},{"issue":"5","key":"7_CR17_7","doi-asserted-by":"crossref","first-page":"8026","DOI":"10.2807\/ese.13.05.08026-en","volume":"13","author":"A Lackenby","year":"2008","unstructured":"Lackenby, A., Hungnes, O., Dudman, S. G., Meijer, A., Paget, W. J., Hay, A. J., and Zambon, M. C. (2008) Emergence of resistance to oseltamivir among influenza A(H1N1) viruses in Europe. Euro. Surveill. 13(5), 8026.","journal-title":"Euro. Surveill."},{"issue":"1","key":"7_CR18_7","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.antiviral.2009.03.003","volume":"83","author":"AC Hurt","year":"2009","unstructured":"Hurt A. C., Ernest J., Deng Y. M., Iannello P., Besselaar T. G., Birch C., Buchy P., Chittaganpitch M., Chiu S. C., Dwyer D., Guigon A., Harrower B., Kei I. P., Kok T., Lin C., McPhie K., Mohd A., Olveda R., Panayotou T., Rawlinson W., Scott L., Smith D., D\u2019Souza H., Komadina N., Shaw R., Kelso A., and Barr I.G. (2009) Emergence and spread of oseltamivir-resistant A(H1N1) influenza viruses in Oceania, South East Asia and South Africa. Antiviral Res. 83(1), 90\u201393.","journal-title":"Antiviral Res."},{"key":"7_CR19_7","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1637\/0005-2086-47.s3.1141","volume":"47","author":"BA Mungall","year":"2003","unstructured":"Mungall, B. A., Xu, X., and Klimov, A. (2003) Assaying susceptibility of avian and other influenza A viruses to zanamivir: comparison of fluorescent and chemiluminescent neuraminidase assays. Avian Dis. 47, 1141\u20131144.","journal-title":"Avian Dis."},{"key":"7_CR20_7","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1128\/JCM.41.2.742-750.2003","volume":"41","author":"NT Wetherall","year":"2003","unstructured":"Wetherall, N. T., Trivedi, T., Zeller, J., Hodges-Savola, C., Kimm-Breschkin, J. L., Zambon, M., and Hayden, F. G. (2003) Evaluation of neuraminidase enzyme assays using different substrates to measure susceptibility of influenza virus clinical isolates to neuraminidase inhibitors: report of the neuraminidase inhibitor susceptibility network. J. Clin. Microbiol. 41, 742\u2013750.","journal-title":"J. Clin. Microbiol."},{"key":"7_CR21_7","doi-asserted-by":"publisher","first-page":"4515","DOI":"10.1128\/AAC.49.11.4515-4520.2005","volume":"49","author":"VP Mishin","year":"2005","unstructured":"Mishin, V. P., Hayden, F. G., and Gubareva, L. V. (2005) Susceptibilities of antiviral-resistant influenza viruses to novel neuraminidase inhibitors. Antimicrob. Agents Chemother. 49, 4515\u20134520.","journal-title":"Antimicrob. Agents Chemother."},{"key":"7_CR22_7","doi-asserted-by":"publisher","first-page":"2264","DOI":"10.1128\/AAC.47.7.2264-2272.2003","volume":"47","author":"J McKimm-Breschkin","year":"2003","unstructured":"McKimm-Breschkin, J., Trivedi, T., Hampson, A., Hay, A., Klimov, A., Tashiro, M., Hayden, F., and Zambon, M. (2003) Neuraminidase sequence analysis and susceptibilities of influenza virus clinical isolates to zanamivir and oseltamivir. Antimicrob. Agents Chemother. 47, 2264\u20132272.","journal-title":"Antimicrob. Agents Chemother."},{"key":"7_CR23_7","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1006\/abio.2000.4517","volume":"280","author":"RC Buxton","year":"2000","unstructured":"Buxton, R. C., Edwards, B., Juo, R. R., Voyta, J. C., Tisdale, M., and Bethell, R. C. (2000) Development of a sensitive chemiluminescent neuraminidase assay for the determination of influenza virus susceptibility to zanamivir. Anal. Biochem. 280, 291\u2013300.","journal-title":"Anal. Biochem."},{"key":"7_CR24_7","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/0003-2697(79)90362-2","volume":"94","author":"M Potier","year":"1979","unstructured":"Potier, M., Mameli, L., Belisle, M., Dallaire, L., and Melancon, S. B. (1979) Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-alpha-D-N-acetylneuraminate) substrate. Anal. Biochem. 94, 287\u2013296.","journal-title":"Anal. Biochem."},{"key":"7_CR25_7","doi-asserted-by":"publisher","first-page":"10366","DOI":"10.1128\/JVI.01200-09","volume":"83","author":"AC Hurt","year":"2009","unstructured":"Hurt A. C., Holien J. K., Parker M., Kelso A., and Barr I. G. (2009) Zanamivir-Resistant Influenza Viruses with a Novel Neuraminidase Mutation. J.Virol. 83, 10366\u201310373.","journal-title":"J. Virol."},{"key":"7_CR26_7","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1016\/j.antiviral.2009.11.005","volume":"85","author":"M Okomo-Adhiambo","year":"2010","unstructured":"Okomo-Adhiambo, M.; Nguyen, H.T.; Sleeman, K.; Sheu, T.G.; Deyde, V.M.; Garten, R.J.; Xu, X.; Shaw, M.W.; Klimov, A.I.; Gubareva, L.V. (2010) Host cell selection of influenza neuraminidase variants: implications for drug resistance monitoring in A(H1N1) viruses. Antiviral Res. 85, 381\u2013388.","journal-title":"Antiviral Res."},{"key":"7_CR27_7","unstructured":"NISN website: http:\/\/www.nisn.org\/v_analysis_guidance_ic50.php (accessed on September 23, 2011)"},{"key":"7_CR28_7","unstructured":"Tocris Website http:\/\/www.tocris.com\/molarityCalculator.php (accessed on September 23, 2011)."},{"key":"7_CR29_7","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.antiviral.2008.08.008","volume":"81","author":"VM Deyde","year":"2009","unstructured":"Deyde, V. M., Okomo-Adhiambo, M., Sheu, T. G., Wallis, T. R., Fry, A., Dharan, N., Klimov, A. I., and Gubareva, L. V. (2009) Pyrosequencing as a tool to detect molecular markers of resistance to neuraminidase inhibitors in seasonal influenza A viruses. Antiviral Res. 81, 16\u201324.","journal-title":"Antiviral Res."},{"key":"7_CR30_7","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1586\/erm.09.21","volume":"9","author":"VM Deyde","year":"2009","unstructured":"Deyde, V. M. and Gubareva, L. V. (2009) Influenza genome analysis using pyrosequencing method: current applications for a moving target. Expert. Rev. Mol. Diagn. 9, 493\u2013509.","journal-title":"Expert. Rev. Mol. Diagn."}],"container-title":["Methods in Molecular Biology","Influenza Virus"],"original-title":[],"link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-61779-621-0_7.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T17:51:01Z","timestamp":1619891461000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-61779-621-0_7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"ISBN":["9781617796203","9781617796210"],"references-count":30,"URL":"https:\/\/doi.org\/10.1007\/978-1-61779-621-0_7","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}