{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T07:06:53Z","timestamp":1743145613503,"version":"3.40.3"},"publisher-location":"Totowa, NJ","reference-count":41,"publisher":"Humana Press","isbn-type":[{"type":"print","value":"9781617798849"},{"type":"electronic","value":"9781617798856"}],"license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012]]},"DOI":"10.1007\/978-1-61779-885-6_14","type":"book-chapter","created":{"date-parts":[[2012,7,18]],"date-time":"2012-07-18T01:16:01Z","timestamp":1342574161000},"page":"201-221","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["SILAC for the Study of Mammalian Cell Lines and Yeast Protein Complexes"],"prefix":"10.1007","author":[{"given":"Heike","family":"Piechura","sequence":"first","affiliation":[]},{"given":"Silke","family":"Oeljeklaus","sequence":"additional","affiliation":[]},{"given":"Bettina","family":"Warscheid","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2012,5,7]]},"reference":[{"key":"14_CR1","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1074\/mcp.M200025-MCP200","volume":"1","author":"SE Ong","year":"2002","unstructured":"Ong SE, Blagoev B, Kratchmarova I et al (2002) Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol Cell Proteomics 1:376\u2013386","journal-title":"Mol Cell Proteomics"},{"key":"14_CR2","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1038\/nbt790","volume":"21","author":"B Blagoev","year":"2003","unstructured":"Blagoev B, Kratchmarova I, Ong SE et al (2003) A proteomics strategy to elucidate functional protein\u2013protein interactions applied to EGF signaling. Nat Biotechnol 21:315\u2013318","journal-title":"Nat Biotechnol"},{"key":"14_CR3","doi-asserted-by":"publisher","first-page":"1674","DOI":"10.1016\/j.febslet.2009.04.018","volume":"583","author":"S Oeljeklaus","year":"2009","unstructured":"Oeljeklaus S, Meyer HE, Warscheid B (2009) New dimensions in the study of protein complexes using quantitative mass spectrometry. FEBS Lett 583:1674\u20131683","journal-title":"FEBS Lett"},{"key":"14_CR4","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1074\/mcp.M700261-MCP200","volume":"7","author":"X Wang","year":"2008","unstructured":"Wang X, Huang L (2008) Identifying dynamic interactors of protein complexes by quantitative mass spectrometry. Mol Cell Proteomics 7:46\u201357","journal-title":"Mol Cell Proteomics"},{"key":"14_CR5","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1074\/mcp.M700306-MCP200","volume":"7","author":"F Mousson","year":"2008","unstructured":"Mousson F, Kolkman A, Pijnappel WW et al (2008) Quantitative proteomics reveals regulation of dynamic components within TATA-binding protein (TBP) transcription complexes. Mol Cell Proteomics 7:845\u2013852","journal-title":"Mol Cell Proteomics"},{"key":"14_CR6","doi-asserted-by":"publisher","first-page":"6043","DOI":"10.1021\/ac034931f","volume":"75","author":"N Ibarrola","year":"2003","unstructured":"Ibarrola N, Kalume DE, Gronborg M et al (2003) A proteomic approach for quantitation of phosphorylation using stable isotope labeling in cell culture. Anal Chem 75:6043\u20136049","journal-title":"Anal Chem"},{"key":"14_CR7","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1074\/mcp.M400219-MCP200","volume":"4","author":"A Gruhler","year":"2005","unstructured":"Gruhler A, Olsen JV, Mohammed S et al (2005) Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway. Mol Cell Proteomics 4:310\u2013327","journal-title":"Mol Cell Proteomics"},{"key":"14_CR8","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1038\/nmeth715","volume":"1","author":"SE Ong","year":"2004","unstructured":"Ong SE, Mittler G, Mann M (2004) Identifying and quantifying in vivo methylation sites by heavy methyl SILAC. Nat Methods 1:119\u2013126","journal-title":"Nat Methods"},{"key":"14_CR9","doi-asserted-by":"publisher","first-page":"710","DOI":"10.1002\/prot.22104","volume":"73","author":"JR Wi\u015bniewski","year":"2008","unstructured":"Wi\u015bniewski JR, Zougman A, Kr\u00fcger S et al (2008) Constitutive and dynamic phosphorylation and acetylation sites on NUCKS, a hypermodified nuclear protein, studied by quantitative proteomics. Proteins 73:710\u2013718","journal-title":"Proteins"},{"key":"14_CR10","doi-asserted-by":"publisher","first-page":"834","DOI":"10.1126\/science.1175371","volume":"325","author":"C Choudhary","year":"2009","unstructured":"Choudhary C, Kumar C, Gnad F et al (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science 325:834\u2013840","journal-title":"Science"},{"key":"14_CR11","doi-asserted-by":"publisher","first-page":"4566","DOI":"10.1021\/pr800468j","volume":"7","author":"D Meierhofer","year":"2008","unstructured":"Meierhofer D, Wang X, Huang L, Kaiser P (2008) Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry. J Proteome Res 7:4566\u20134576","journal-title":"J Proteome Res"},{"key":"14_CR12","doi-asserted-by":"publisher","first-page":"1751","DOI":"10.1074\/mcp.M800588-MCP200","volume":"8","author":"FS Oppermann","year":"2009","unstructured":"Oppermann FS, Gnad F, Olsen JV et al (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8:1751\u20131764","journal-title":"Mol Cell Proteomics"},{"issue":"104","key":"14_CR13","doi-asserted-by":"crossref","first-page":"ra3","DOI":"10.1126\/scisignal.2000475","volume":"3","author":"JV Olsen","year":"2010","unstructured":"Olsen JV, Vermeulen M, Santamaria A et al (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3(104):ra3","journal-title":"Sci Signal"},{"key":"14_CR14","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1074\/mcp.M500241-MCP200","volume":"5","author":"E Milner","year":"2006","unstructured":"Milner E, Barnea E, Beer I, Admon A (2006) The turnover kinetics of major histocompatibility complex peptides of human cancer cells. Mol Cell Proteomics 5:357\u2013365","journal-title":"Mol Cell Proteomics"},{"key":"14_CR15","doi-asserted-by":"publisher","first-page":"762","DOI":"10.1016\/j.molcel.2008.07.018","volume":"31","author":"T Bonaldi","year":"2008","unstructured":"Bonaldi T, Straub T, Cox J et al (2008) Combined use of RNAi and quantitative proteomics to study gene function in Drosophila. Mol Cell 31:762\u2013772","journal-title":"Mol Cell"},{"key":"14_CR16","doi-asserted-by":"publisher","first-page":"3638","DOI":"10.1021\/pr100150w","volume":"9","author":"B Soufi","year":"2010","unstructured":"Soufi B, Kumar C, Gnad F et al (2010) Stable isotope labeling by amino acids in cell culture (SILAC) applied to quantitative proteomics of Bacillus subtilis. J Proteome Res 9:3638\u20133646","journal-title":"J Proteome Res"},{"key":"14_CR17","doi-asserted-by":"publisher","first-page":"1697","DOI":"10.1074\/mcp.M500190-MCP200","volume":"4","author":"A Gruhler","year":"2005","unstructured":"Gruhler A, Schulze WX, Matthiesen R et al (2005) Stable isotope labeling of Arabidopsis thaliana cells and quantitative proteomics by mass spectrometry. Mol Cell Proteomics 4:1697\u20131709","journal-title":"Mol Cell Proteomics"},{"key":"14_CR18","doi-asserted-by":"publisher","first-page":"2173","DOI":"10.1074\/mcp.M110.000323","volume":"9","author":"MD Sury","year":"2010","unstructured":"Sury MD, Chen JX, Selbach M (2010) The SILAC fly allows for accurate protein quantification in vivo. Mol Cell Proteomics 9:2173\u20132183","journal-title":"Mol Cell Proteomics"},{"key":"14_CR19","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.cell.2008.05.033","volume":"134","author":"M Kr\u00fcger","year":"2008","unstructured":"Kr\u00fcger M, Moser M, Ussar S et al (2008) SILAC mouse for quantitative proteomics uncovers kindlin-3 as an essential factor for red blood cell function. Cell 134:353\u2013364","journal-title":"Cell"},{"key":"14_CR20","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1021\/pr0255708","volume":"2","author":"SE Ong","year":"2003","unstructured":"Ong SE, Kratchmarova I, Mann M (2003) Properties of 13C-substituted arginine instable isotope labeling by amino acids in cell culture (SILAC). J Proteome Res 2:173\u2013181","journal-title":"J Proteome Res"},{"key":"14_CR21","doi-asserted-by":"publisher","first-page":"1587","DOI":"10.1074\/mcp.M800113-MCP200","volume":"7","author":"SC Bendall","year":"2008","unstructured":"Bendall SC, Hughes C, Stewart MH et al (2008) Prevention of amino acid conversion in SILAC experiments with embryonic stem cells. Mol Cell Proteomics 7:1587\u20131597","journal-title":"Mol Cell Proteomics"},{"key":"14_CR22","doi-asserted-by":"publisher","first-page":"1567","DOI":"10.1074\/mcp.M110.000208","volume":"9","author":"CC Bicho","year":"2010","unstructured":"Bicho CC, de Lima AF, Chen ZA et al (2010) A genetic engineering solution to the \u201carginine conversion problem\u201d in stable isotope labeling by amino acids in cell culture (SILAC). Mol Cell Proteomics 9:1567\u20131577","journal-title":"Mol Cell Proteomics"},{"key":"14_CR23","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1038\/nchembio736","volume":"1","author":"SE Ong","year":"2005","unstructured":"Ong SE, Mann M (2005) Mass spectrometry-based proteomics turns quantitative. Nat Chem Biol 1:252\u2013262","journal-title":"Nat Chem Biol"},{"key":"14_CR24","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1074\/mcp.R400010-MCP200","volume":"4","author":"RJ Beynon","year":"2005","unstructured":"Beynon RJ, Pratt JM (2005) Metabolic labeling of proteins for proteomics. Mol Cell Proteomics 4:857\u2013872","journal-title":"Mol Cell Proteomics"},{"key":"14_CR25","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1021\/pr800650r","volume":"8","author":"H Molina","year":"2009","unstructured":"Molina H, Yang Y, Ruch T et al (2009) Temporal profiling of the adipocyte proteome during differentiation using a five-plex SILAC based strategy. J Proteome Res 8:48\u201358","journal-title":"J Proteome Res"},{"key":"14_CR26","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1021\/pr101065j","volume":"10","author":"J Cox","year":"2011","unstructured":"Cox J, Neuhauser N, Michalski A, Scheltema RA et al (2011) Andromeda: a peptide search engine integrated into the MaxQuant environment. J Proteome Res 10:1794\u20131805","journal-title":"J Proteome Res"},{"key":"14_CR27","doi-asserted-by":"publisher","first-page":"2650","DOI":"10.1038\/nprot.2006.427","volume":"1","author":"SE Ong","year":"2006","unstructured":"Ong SE, Mann M (2006) A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC). Nat Protoc 1:2650\u20132660","journal-title":"Nat Protoc"},{"key":"14_CR28","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/j.ymeth.2006.08.001","volume":"40","author":"B Blagoev","year":"2006","unstructured":"Blagoev B, Mann M (2006) Quantitative proteomics to study mitogen-activated protein kinases. Methods 40:243\u2013250","journal-title":"Methods"},{"key":"14_CR29","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.ab.2011.01.011","volume":"412","author":"C L\u00f6\u00dfner","year":"2011","unstructured":"L\u00f6\u00dfner C, Warnken U, Pscherer A, Schn\u00f6lzer M (2011) Preventing arginine-to-proline conversion in a cell line independent manner during cell cultivation under SILAC conditions. Anal Biochem 412:123\u2013125","journal-title":"Anal Biochem"},{"key":"14_CR30","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248\u2013254","journal-title":"Anal Biochem"},{"key":"14_CR31","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.1038\/nbt.1511","volume":"26","author":"J Cox","year":"2008","unstructured":"Cox J, Mann M (2008) MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. Nat Biotechnol 26:1367\u20131372","journal-title":"Nat Biotechnol"},{"key":"14_CR32","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/nprot.2009.36","volume":"4","author":"J Cox","year":"2009","unstructured":"Cox J, Matic I, Hilger M et al (2009) A practical guide to the MaxQuant computational platform for SILAC-based quantitative proteomics. Nat Protoc 4:698\u2013705","journal-title":"Nat Protoc"},{"key":"14_CR33","doi-asserted-by":"publisher","first-page":"1118","DOI":"10.1021\/pr7007175","volume":"7","author":"S Hanke","year":"2008","unstructured":"Hanke S, Besir H, Oesterhelt D, Mann M (2008) Absolute SILAC for accurate quantitation of proteins in complex mixtures down to the attomole level. J Proteome Res 7:1118\u20131130","journal-title":"J Proteome Res"},{"key":"14_CR34","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1002\/pmic.200800275","volume":"9","author":"B Schwanh\u00e4usser","year":"2009","unstructured":"Schwanh\u00e4usser B, Gossen M, Dittmar G, Selbach M (2009) Global analysis of cellular protein translation by pulsed SILAC. Proteomics 9:205\u2013209","journal-title":"Proteomics"},{"key":"14_CR35","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1021\/pr800641v","volume":"8","author":"MK Doherty","year":"2009","unstructured":"Doherty MK, Hammond DE, Clague MJ et al (2009) Turnover of the human proteome: determination of protein intracellular stability by dynamic SILAC. J Proteome Res 8:104\u2013112","journal-title":"J Proteome Res"},{"key":"14_CR36","doi-asserted-by":"publisher","first-page":"2796","DOI":"10.1074\/mcp.M900285-MCP200","volume":"8","author":"C Pan","year":"2009","unstructured":"Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8:2796\u20132808","journal-title":"Mol Cell Proteomics"},{"key":"14_CR37","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1038\/nmeth.1446","volume":"7","author":"T Geiger","year":"2010","unstructured":"Geiger T, Cox J, Ostasiewicz P et al (2010) Super-SILAC mix for quantitative proteomics of human tumor tissue. Nat Methods 7:383\u2013385","journal-title":"Nat Methods"},{"key":"14_CR38","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1016\/j.cell.2010.10.012","volume":"143","author":"T Bartke","year":"2010","unstructured":"Bartke T, Vermeulen M, Xhemalce B et al (2010) Nucleosome-interacting proteins regulated by DNA and histone methylation. Cell 143:470\u2013484","journal-title":"Cell"},{"key":"14_CR39","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1038\/nmeth.1513","volume":"7","author":"O Rechavi","year":"2010","unstructured":"Rechavi O, Kalman M, Fang Y et al (2010) Trans-SILAC: sorting out the non-cell-autonomous proteome. Nat Methods 7:923\u2013927","journal-title":"Nat Methods"},{"key":"14_CR40","doi-asserted-by":"publisher","first-page":"1541","DOI":"10.1002\/(SICI)1097-0061(199910)15:14<1541::AID-YEA476>3.0.CO;2-K","volume":"15","author":"AL Goldstein","year":"1999","unstructured":"Goldstein AL, McCusker JH (1999) Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 15:1541\u20131553","journal-title":"Yeast"},{"key":"14_CR41","doi-asserted-by":"publisher","first-page":"963","DOI":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W","volume":"15","author":"M Knop","year":"1999","unstructured":"Knop M, Siegers K, Pereira G et al (1999) Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines. Yeast 15:963\u2013972","journal-title":"Yeast"}],"container-title":["Methods in Molecular Biology","Quantitative Methods in Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-61779-885-6_14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,19]],"date-time":"2023-02-19T02:50:14Z","timestamp":1676775014000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-61779-885-6_14"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"ISBN":["9781617798849","9781617798856"],"references-count":41,"URL":"https:\/\/doi.org\/10.1007\/978-1-61779-885-6_14","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"type":"print","value":"1064-3745"},{"type":"electronic","value":"1940-6029"}],"subject":[],"published":{"date-parts":[[2012]]},"assertion":[{"value":"7 May 2012","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}