{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T07:18:47Z","timestamp":1792221527874,"version":"4.4.0"},"publisher-location":"Totowa, NJ","reference-count":56,"publisher":"Humana Press","isbn-type":[{"value":"9781627032384","type":"print"},{"value":"9781627032391","type":"electronic"}],"license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013]]},"DOI":"10.1007\/978-1-62703-239-1_22","type":"book-chapter","created":{"date-parts":[[2013,1,7]],"date-time":"2013-01-07T15:30:03Z","timestamp":1357572603000},"page":"327-339","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Methods to Investigate the Role of SIRT1 in Endothelial Senescence"],"prefix":"10.1007","author":[{"given":"Bo","family":"Bai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,11,1]]},"reference":[{"key":"22_CR00221","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1016\/0014-4827(61)90192-6","volume":"25","author":"L Hayflick","year":"1961","unstructured":"Hayflick L, Moorhead PS (1961) The serial cultivation of human diploid cell strains. Exp Cell Res 25:585\u2013621","journal-title":"Exp Cell Res"},{"key":"22_CR00222","doi-asserted-by":"publisher","first-page":"11190","DOI":"10.1073\/pnas.92.24.11190","volume":"92","author":"E Chang","year":"1995","unstructured":"Chang E, Harley CB (1995) Telomere length and replicative aging in human vascular tissues. Proc Natl Acad Sci U S A 92:11190\u201311194","journal-title":"Proc Natl Acad Sci U S A"},{"key":"22_CR00223","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1006\/excr.2001.5182","volume":"265","author":"QM Chen","year":"2001","unstructured":"Chen QM, Prowse KR, Tu VC, Purdom S, Linskens MH (2001) Uncoupling the senescent phenotype from telomere shortening in hydrogen peroxide-treated fibroblasts. Exp Cell Res 265:294\u2013303","journal-title":"Exp Cell Res"},{"key":"22_CR00224","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1111\/j.1749-6632.2000.tb06638.x","volume":"908","author":"O Toussaint","year":"2000","unstructured":"Toussaint O, Dumont P, Dierick JF, Pascal T, Frippiat C, Chainiaux F, Sluse F, Eliaers F, Remacle J (2000) Stress-induced premature senescence. Essence of life, evolution, stress, and aging. Ann N Y Acad Sci 908:85\u201398","journal-title":"Ann N Y Acad Sci"},{"key":"22_CR00225","doi-asserted-by":"publisher","first-page":"2888","DOI":"10.1101\/gad.9.23.2888","volume":"9","author":"CB Brachmann","year":"1995","unstructured":"Brachmann CB, Sherman JM, Devine SE, Cameron EE, Pillus L, Boeke JD (1995) The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability. Genes Dev 9:2888\u20132902","journal-title":"Genes Dev"},{"key":"22_CR00226","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1101\/gad.7.4.592","volume":"7","author":"M Braunstein","year":"1993","unstructured":"Braunstein M, Rose AB, Holmes SG, Allis CD, Broach JR (1993) Transcriptional silencing in yeast is associated with reduced nucleosome acetylation. Genes Dev 7:592\u2013604","journal-title":"Genes Dev"},{"key":"22_CR00227","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1016\/0092-8674(89)90681-8","volume":"56","author":"S Gottlieb","year":"1989","unstructured":"Gottlieb S, Esposito RE (1989) A new role for a yeast transcriptional silencer gene, SIR2, in regulation of recombination in ribosomal DNA. Cell 56:771\u2013776","journal-title":"Cell"},{"key":"22_CR00228","doi-asserted-by":"crossref","first-page":"2817","DOI":"10.1002\/j.1460-2075.1984.tb02214.x","volume":"3","author":"D Shore","year":"1984","unstructured":"Shore D, Squire M, Nasmyth KA (1984) Characterization of two genes required for the position-effect control of yeast mating-type genes. EMBO J 3:2817\u20132823","journal-title":"EMBO J"},{"key":"22_CR00229","doi-asserted-by":"publisher","first-page":"1279","DOI":"10.1016\/0092-8674(91)90049-5","volume":"66","author":"OM Aparicio","year":"1991","unstructured":"Aparicio OM, Billington BL, Gottschling DE (1991) Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae. Cell 66:1279\u20131287","journal-title":"Cell"},{"key":"22_CR002210","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1101\/gad.11.2.241","volume":"11","author":"JS Smith","year":"1997","unstructured":"Smith JS, Boeke JD (1997) An unusual form of transcriptional silencing in yeast ribosomal DNA. Genes Dev 11:241\u2013254","journal-title":"Genes Dev"},{"key":"22_CR002211","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1093\/genetics\/93.4.877","volume":"93","author":"J Rine","year":"1979","unstructured":"Rine J, Strathern JN, Hicks JB, Herskowitz I (1979) A suppressor of mating-type locus mutations in Saccharomyces cerevisiae: evidence for and identification of cryptic mating-type loci. Genetics 93:877\u2013901","journal-title":"Genetics"},{"key":"22_CR002212","doi-asserted-by":"publisher","first-page":"2235","DOI":"10.1056\/NEJMra1100831","volume":"364","author":"L Guarente","year":"2011","unstructured":"Guarente L (2011) Franklin H. Epstein Lecture: sirtuins, aging, and medicine. N Engl J Med 364:2235\u20132244","journal-title":"N Engl J Med"},{"key":"22_CR002213","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1146\/annurev.pathol.4.110807.092250","volume":"5","author":"MC Haigis","year":"2010","unstructured":"Haigis MC, Sinclair DA (2010) Mammalian sirtuins: biological insights and disease relevance. Annu Rev Pathol 5:253\u2013295","journal-title":"Annu Rev Pathol"},{"key":"22_CR002214","doi-asserted-by":"publisher","first-page":"2570","DOI":"10.1101\/gad.13.19.2570","volume":"13","author":"M Kaeberlein","year":"1999","unstructured":"Kaeberlein M, McVey M, Guarente L (1999) The SIR2\/3\/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev 13:2570\u20132580","journal-title":"Genes Dev"},{"key":"22_CR002215","doi-asserted-by":"publisher","first-page":"15998","DOI":"10.1073\/pnas.0404184101","volume":"101","author":"B Rogina","year":"2004","unstructured":"Rogina B, Helfand SL (2004) Sir2 mediates longevity in the fly through a pathway related to calorie restriction. Proc Natl Acad Sci U S A 101:15998\u201316003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"22_CR002216","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1038\/35065638","volume":"410","author":"HA Tissenbaum","year":"2001","unstructured":"Tissenbaum HA, Guarente L (2001) Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans. Nature 410:227\u2013230","journal-title":"Nature"},{"key":"22_CR002217","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.mad.2005.09.005","volume":"127","author":"Y Wang","year":"2006","unstructured":"Wang Y, Tissenbaum HA (2006) Overlapping and distinct functions for a Caenorhabditis elegans SIR2 and DAF-16\/FOXO. Mech Ageing Dev 127:48\u201356","journal-title":"Mech Ageing Dev"},{"key":"22_CR002218","doi-asserted-by":"publisher","first-page":"686","DOI":"10.1038\/nature02789","volume":"430","author":"JG Wood","year":"2004","unstructured":"Wood JG, Rogina B, Lavu S, Howitz K, Helfand SL, Tatar M, Sinclair D (2004) Sirtuin activators mimic caloric restriction and delay ageing in metazoans. Nature 430:686\u2013689","journal-title":"Nature"},{"key":"22_CR002219","doi-asserted-by":"publisher","first-page":"1427","DOI":"10.1091\/mbc.01-10-0482","volume":"13","author":"CM Armstrong","year":"2002","unstructured":"Armstrong CM, Kaeberlein M, Imai SI, Guarente L (2002) Mutations in Saccharomyces cerevisiae gene SIR2 can have differential effects on in vivo silencing phenotypes and in vitro histone deacetylation activity. Mol Biol Cell 13:1427\u20131438","journal-title":"Mol Biol Cell"},{"key":"22_CR002220","doi-asserted-by":"publisher","first-page":"939","DOI":"10.1189\/jlb.0903424","volume":"75","author":"SW Buck","year":"2004","unstructured":"Buck SW, Gallo CM, Smith JS (2004) Diversity in the Sir2 family of protein deacetylases. J Leukoc Biol 75:939\u2013950","journal-title":"J Leukoc Biol"},{"key":"22_CR002221","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1006\/bbrc.2000.3000","volume":"273","author":"RA Frye","year":"2000","unstructured":"Frye RA (2000) Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins. Biochem Biophys Res Commun 273:793\u2013798","journal-title":"Biochem Biophys Res Commun"},{"key":"22_CR002222","doi-asserted-by":"publisher","first-page":"e7350","DOI":"10.1371\/journal.pone.0007350","volume":"4","author":"I Autiero","year":"2009","unstructured":"Autiero I, Costantini S, Colonna G (2009) Human sirt-1: molecular modeling and structure-function relationships of an unordered protein. PLoS One 4:e7350","journal-title":"PLoS One"},{"key":"22_CR002223","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1007\/s10822-006-9084-9","volume":"20","author":"T Huhtiniemi","year":"2006","unstructured":"Huhtiniemi T, Wittekindt C, Laitinen T, Leppanen J, Salminen A, Poso A, Lahtela-Kakkonen M (2006) Comparative and pharmacophore model for deacetylase SIRT1. J Comput Aided Mol Des 20:589\u2013599","journal-title":"J Comput Aided Mol Des"},{"key":"22_CR002224","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1038\/nature08197","volume":"460","author":"T Finkel","year":"2009","unstructured":"Finkel T, Deng CX, Mostoslavsky R (2009) Recent progress in the biology and physiology of sirtuins. Nature 460:587\u2013591","journal-title":"Nature"},{"key":"22_CR002225","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1126\/science.1099196","volume":"305","author":"HY Cohen","year":"2004","unstructured":"Cohen HY, Miller C, Bitterman KJ, Wall NR, Hekking B, Kessler B, Howitz KT, Gorospe M, de Cabo R, Sinclair DA (2004) Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. Science 305:390\u2013392","journal-title":"Science"},{"key":"22_CR002226","doi-asserted-by":"publisher","first-page":"1731","DOI":"10.1126\/science.304.5678.1731a","volume":"304","author":"J Couzin","year":"2004","unstructured":"Couzin J (2004) Research on aging. Gene links calorie deprivation and long life in rodents. Science 304:1731","journal-title":"Science"},{"key":"22_CR002227","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1016\/j.cell.2005.01.029","volume":"120","author":"L Guarente","year":"2005","unstructured":"Guarente L, Picard F (2005) Calorie restriction\u2013the SIR2 connection. Cell 120:473\u2013482","journal-title":"Cell"},{"key":"22_CR002228","doi-asserted-by":"publisher","first-page":"1641","DOI":"10.1126\/science.1118357","volume":"310","author":"D Chen","year":"2005","unstructured":"Chen D, Steele AD, Lindquist S, Guarente L (2005) Increase in activity during calorie restriction requires Sirt1. Science 310:1641","journal-title":"Science"},{"key":"22_CR002229","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/j.cmet.2008.08.014","volume":"8","author":"AS Banks","year":"2008","unstructured":"Banks AS, Kon N, Knight C, Matsumoto M, Gutierrez-Juarez R, Rossetti L, Gu W, Accili D (2008) SirT1 gain of function increases energy efficiency and prevents diabetes in mice. Cell Metab 8:333\u2013341","journal-title":"Cell Metab"},{"key":"22_CR002230","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.cell.2006.07.002","volume":"126","author":"VD Longo","year":"2006","unstructured":"Longo VD, Kennedy BK (2006) Sirtuins in aging and age-related disease. Cell 126:257\u2013268","journal-title":"Cell"},{"key":"22_CR002231","doi-asserted-by":"publisher","first-page":"e1759","DOI":"10.1371\/journal.pone.0001759","volume":"3","author":"G Boily","year":"2008","unstructured":"Boily G, Seifert EL, Bevilacqua L, He XH, Sabourin G, Estey C, Moffat C, Crawford S, Saliba S, Jardine K, Xuan J, Evans M, Harper M-E, McBurney MW (2008) SirT1 Regulates energy metabolism and response to caloric restriction in mice. PLoS One 3:e1759","journal-title":"PLoS One"},{"key":"22_CR002232","doi-asserted-by":"publisher","first-page":"868","DOI":"10.1038\/nature05486","volume":"444","author":"L Guarente","year":"2006","unstructured":"Guarente L (2006) Sirtuins as potential targets for metabolic syndrome. Nature 444:868","journal-title":"Nature"},{"key":"22_CR002233","doi-asserted-by":"publisher","first-page":"10230","DOI":"10.1073\/pnas.0604392103","volume":"103","author":"WC Hallows","year":"2006","unstructured":"Hallows WC, Lee S, Denu JM (2006) Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases. Proc Natl Acad Sci U S A 103:10230\u201310235","journal-title":"Proc Natl Acad Sci U S A"},{"key":"22_CR002234","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/j.cmet.2007.11.006","volume":"7","author":"B Schwer","year":"2008","unstructured":"Schwer B, Verdin E (2008) Conserved metabolic regulatory functions of sirtuins. Cell Metab 7:104\u2013112","journal-title":"Cell Metab"},{"key":"22_CR002235","doi-asserted-by":"publisher","first-page":"2383","DOI":"10.1093\/emboj\/21.10.2383","volume":"21","author":"E Langley","year":"2002","unstructured":"Langley E, Pearson M, Faretta M, Bauer UM, Frye RA, Minucci S, Pelicci PG, Kouzarides T (2002) Human SIR2 deacetylates p53 and antagonizes PML\/p53-induced cellular senescence. EMBO J 21:2383\u20132396","journal-title":"EMBO J"},{"key":"22_CR002236","doi-asserted-by":"publisher","first-page":"1478","DOI":"10.4161\/cc.8.10.8408","volume":"8","author":"B Jung-Hynes","year":"2009","unstructured":"Jung-Hynes B, Ahmad N (2009) Role of p53 in the anti-proliferative effects of Sirt1 inhibition in prostate cancer cells. Cell Cycle 8:1478\u20131483","journal-title":"Cell Cycle"},{"key":"22_CR002237","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/sj.onc.1209049","volume":"25","author":"H Ota","year":"2006","unstructured":"Ota H, Tokunaga E, Chang K, Hikasa M, Iijima K, Eto M, Kozaki K, Akishita M, Ouchi Y, Kaneki M (2006) Sirt1 inhibitor, Sirtinol, induces senescence-like growth arrest with attenuated Ras-MAPK signaling in human cancer cells. Oncogene 25:176\u2013185","journal-title":"Oncogene"},{"key":"22_CR002238","doi-asserted-by":"publisher","first-page":"e1710","DOI":"10.1371\/journal.pone.0001710","volume":"3","author":"J Huang","year":"2008","unstructured":"Huang J, Gan Q, Han L, Li J, Zhang H, Sun Y, Zhang Z, Tong T (2008) SIRT1 overexpression antagonizes cellular senescence with activated ERK\/S6k1 signaling in human diploid fibroblasts. PLoS One 3:e1710","journal-title":"PLoS One"},{"key":"22_CR002239","doi-asserted-by":"publisher","first-page":"1634","DOI":"10.1161\/ATVBAHA.108.164368","volume":"28","author":"H Ota","year":"2008","unstructured":"Ota H, Eto M, Kano MR, Ogawa S, Iijima K, Akishita M, Ouchi Y (2008) Cilostazol inhibits oxidative stress-induced premature senescence via upregulation of Sirt1 in human endothelial cells. Arterioscler Thromb Vasc Biol 28:1634\u20131639","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"22_CR002240","doi-asserted-by":"publisher","first-page":"1384","DOI":"10.1161\/CIRCRESAHA.109.215483","volume":"106","author":"Y Zu","year":"2010","unstructured":"Zu Y, Liu L, Lee MY, Xu C, Liang Y, Man RY, Vanhoutte PM, Wang Y (2010) SIRT1 promotes proliferation and prevents senescence through targeting LKB1 in primary porcine aortic endothelial cells. Circ Res 106:1384\u20131393","journal-title":"Circ Res"},{"key":"22_CR002241","doi-asserted-by":"publisher","first-page":"2054","DOI":"10.1161\/ATVBAHA.110.216739","volume":"31","author":"A Takemura","year":"2011","unstructured":"Takemura A, Iijima K, Ota H, Son BK, Ito Y, Ogawa S, Eto M, Akishita M, Ouchi Y (2011) Sirtuin 1 retards hyperphosphatemia-induced calcification of vascular smooth muscle cells. Arterioscler Thromb Vasc Biol 31:2054\u20132062","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"22_CR002242","doi-asserted-by":"publisher","first-page":"936","DOI":"10.1016\/j.bbapap.2008.03.004","volume":"1784","author":"ML Balestrieri","year":"2008","unstructured":"Balestrieri ML, Rienzo M, Felice F, Rossiello R, Grimaldi V, Milone L, Casamassimi A, Servillo L, Farzati B, Giovane A, Napoli C (2008) High glucose downregulates endothelial progenitor cell number via SIRT1. Biochim Biophys Acta 1784:936\u2013945","journal-title":"Biochim Biophys Acta"},{"key":"22_CR002243","doi-asserted-by":"publisher","first-page":"E110","DOI":"10.1152\/ajpendo.00192.2010","volume":"299","author":"T Zhao","year":"2010","unstructured":"Zhao T, Li J, Chen AF (2010) MicroRNA-34a induces endothelial progenitor cell senescence and impedes its angiogenesis via suppressing silent information regulator 1. Am J Physiol Endocrinol Metab 299:E110\u2013E116","journal-title":"Am J Physiol Endocrinol Metab"},{"issue":"10","key":"22_CR002244","doi-asserted-by":"publisher","first-page":"1749","DOI":"10.1089\/scd.2011.0429","volume":"21","author":"YJ Kim","year":"2012","unstructured":"Kim YJ, Hwang SH, Lee SY, Shin KK, Cho HH, Bae YC, Jung JS (2012) miR-486-5p induces replicative senescence of human adipose tissue-derived mesenchymal stem cells and its expression Is controlled by high glucose. Stem Cells Dev 21(10):1749\u20131760","journal-title":"Stem Cells Dev"},{"key":"22_CR002245","doi-asserted-by":"publisher","first-page":"986","DOI":"10.1016\/j.febslet.2010.11.047","volume":"585","author":"Y Wang","year":"2011","unstructured":"Wang Y, Liang Y, Vanhoutte PM (2011) SIRT1 and AMPK in regulating mammalian senescence: a critical review and a working model. FEBS Lett 585:986\u2013994","journal-title":"FEBS Lett"},{"key":"22_CR002246","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1159\/000265563","volume":"47","author":"MY Lee","year":"2010","unstructured":"Lee MY, Wang Y, Vanhoutte PM (2010) Senescence of cultured porcine coronary arterial endothelial cells is associated with accelerated oxidative stress and activation of NFkB. J Vasc Res 47:287\u2013298","journal-title":"J Vasc Res"},{"key":"22_CR002247","doi-asserted-by":"publisher","first-page":"2443","DOI":"10.1161\/ATVBAHA.107.141705","volume":"27","author":"MYK Lee","year":"2007","unstructured":"Lee MYK, Tse HF, Siu CW, Zhu SG, Man RYK, Vanhoutte PM (2007) Genomic changes in regenerated porcine coronary arterial endothelial cells. Arterioscler Thromb Vasc Biol 27:2443\u20132449","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"22_CR002248","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/0003-2697(86)90314-3","volume":"159","author":"RJ Gillies","year":"1986","unstructured":"Gillies RJ, Didier N, Denton M (1986) Determination of cell number in monolayer cultures. Anal Biochem 159:109\u2013113","journal-title":"Anal Biochem"},{"key":"22_CR002249","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/0003-2697(89)90710-0","volume":"182","author":"W Kueng","year":"1989","unstructured":"Kueng W, Silber E, Eppenberger U (1989) Quantification of cells cultured on 96-well plates. Anal Biochem 182:16\u201319","journal-title":"Anal Biochem"},{"key":"22_CR002250","doi-asserted-by":"publisher","first-page":"11462","DOI":"10.1158\/0008-5472.CAN-06-1969","volume":"66","author":"Y Wang","year":"2006","unstructured":"Wang Y, Lam JB, Lam KS, Liu J, Lam MC, Hoo RL, Wu D, Cooper GJ, Xu A (2006) Adiponectin modulates the glycogen synthase kinase-3beta\/beta-catenin signaling pathway and attenuates mammary tumorigenesis of MDA-MB-231 cells in nude mice. Cancer Res 66:11462\u201311470","journal-title":"Cancer Res"},{"key":"22_CR002251","doi-asserted-by":"publisher","first-page":"1798","DOI":"10.1038\/nprot.2009.191","volume":"4","author":"F Debacq-Chainiaux","year":"2009","unstructured":"Debacq-Chainiaux F, Erusalimsky JD, Campisi J, Toussaint O (2009) Protocols to detect senescence-associated beta-galactosidase (SA-betagal) activity, a biomarker of senescent cells in culture and in vivo. Nat Protoc 4:1798\u20131806","journal-title":"Nat Protoc"},{"key":"22_CR002252","doi-asserted-by":"publisher","first-page":"9363","DOI":"10.1073\/pnas.92.20.9363","volume":"92","author":"GP Dimri","year":"1995","unstructured":"Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Linskens M, Rubelj I, Pereira-Smith O et al (1995) A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci U S A 92:9363\u20139367","journal-title":"Proc Natl Acad Sci U S A"},{"key":"22_CR002253","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1111\/j.1474-9726.2006.00199.x","volume":"5","author":"BY Lee","year":"2006","unstructured":"Lee BY, Han JA, Im JS, Morrone A, Johung K, Goodwin EC, Kleijer WJ, DiMaio D, Hwang ES (2006) Senescence-associated beta-galactosidase is lysosomal beta-galactosidase. Aging Cell 5:187\u2013195","journal-title":"Aging Cell"},{"key":"22_CR002254","doi-asserted-by":"publisher","first-page":"2004","DOI":"10.1016\/j.cub.2003.10.031","volume":"13","author":"A Woods","year":"2003","unstructured":"Woods A, Johnstone SR, Dickerson K, Leiper FC, Fryer LG, Neumann D, Schlattner U, Wallimann T, Carlson M, Carling D (2003) LKB1 is the upstream kinase in the AMP-activated protein kinase cascade. Curr Biol 13:2004\u20132008","journal-title":"Curr Biol"},{"key":"22_CR002255","doi-asserted-by":"publisher","first-page":"3569","DOI":"10.1016\/j.febslet.2011.10.022","volume":"585","author":"HJ Kim","year":"2011","unstructured":"Kim HJ, Cho JH, Quan H, Kim JR (2011) Down-regulation of Aurora B kinase induces cellular senescence in human fibroblasts and endothelial cells through a p53-dependent pathway. FEBS Lett 585:3569\u20133576","journal-title":"FEBS Lett"},{"key":"22_CR002256","doi-asserted-by":"publisher","first-page":"H1673","DOI":"10.1152\/ajpheart.00455.2009","volume":"297","author":"R Nakano-Kurimoto","year":"2009","unstructured":"Nakano-Kurimoto R, Ikeda K, Uraoka M, Nakagawa Y, Yutaka K, Koide M, Takahashi T, Matoba S, Yamada H, Okigaki M, Matsubara H (2009) Replicative senescence of vascular smooth muscle cells enhances the calcification through initiating the osteoblastic transition. Am J Physiol Heart Circ Physiol 297:H1673\u2013H1684","journal-title":"Am J Physiol Heart Circ Physiol"}],"container-title":["Methods in Molecular Biology","Cell Senescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-62703-239-1_22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T11:09:58Z","timestamp":1676286598000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-62703-239-1_22"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,1]]},"ISBN":["9781627032384","9781627032391"],"references-count":56,"URL":"https:\/\/doi.org\/10.1007\/978-1-62703-239-1_22","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,1]]},"assertion":[{"value":"1 November 2012","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}