{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T06:42:40Z","timestamp":1761892960265,"version":"3.40.3"},"publisher-location":"Totowa, NJ","reference-count":32,"publisher":"Humana Press","isbn-type":[{"type":"print","value":"9781627033015"},{"type":"electronic","value":"9781627033022"}],"license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013]]},"DOI":"10.1007\/978-1-62703-302-2_24","type":"book-chapter","created":{"date-parts":[[2013,3,25]],"date-time":"2013-03-25T16:23:55Z","timestamp":1364228635000},"page":"431-443","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Immunomagnetic Purification of Fluorescent Legionella-Containing Vacuoles"],"prefix":"10.1007","author":[{"given":"Ivo","family":"Finsel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hubert","family":"Hilbi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,2,8]]},"reference":[{"key":"24_CR00241","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1016\/0966-842X(96)10041-X","volume":"4","author":"BS Fields","year":"1996","unstructured":"Fields BS (1996) The molecular ecology of legionellae. Trends Microbiol 4:286\u2013290","journal-title":"Trends Microbiol"},{"key":"24_CR00242","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1111\/j.1758-2229.2011.00247.x","volume":"3","author":"H Hilbi","year":"2011","unstructured":"Hilbi H, Hoffmann C, Harrison CF (2011) Legionella spp. outdoors: colonization, communication and persistence. Environ Microbiol Rep 3:286\u2013296","journal-title":"Environ Microbiol Rep"},{"key":"24_CR00243","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1128\/CMR.00052-09","volume":"23","author":"HJ Newton","year":"2010","unstructured":"Newton HJ, Ang DK, van Driel IR, Hartland EL (2010) Molecular pathogenesis of infections caused by Legionella pneumophila. Clin Microbiol Rev 23:274\u2013298","journal-title":"Clin Microbiol Rev"},{"key":"24_CR00244","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1111\/j.1462-5822.2005.00493.x","volume":"7","author":"M Steinert","year":"2005","unstructured":"Steinert M, Heuner K (2005) Dictyostelium as host model for pathogenesis. Cell Microbiol 7:307\u2013314","journal-title":"Cell Microbiol"},{"key":"24_CR00245","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1111\/j.1462-2920.2007.01238.x","volume":"9","author":"H Hilbi","year":"2007","unstructured":"Hilbi H, Weber SS, Ragaz C, Nyfeler Y, Urwyler S (2007) Environmental predators as models for bacterial pathogenesis. Environ Microbiol 9:563\u2013575","journal-title":"Environ Microbiol"},{"key":"24_CR00246","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1038\/nrmicro1967","volume":"7","author":"RR Isberg","year":"2009","unstructured":"Isberg RR, O\u2019Connor TJ, Heidtman M (2009) The Legionella pneumophila replication vacuole: making a cosy niche inside host cells. Nat Rev Microbiol 7:13\u201324","journal-title":"Nat Rev Microbiol"},{"key":"24_CR00247","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1111\/j.1600-0854.2012.01344.x","volume":"13","author":"H Hilbi","year":"2012","unstructured":"Hilbi H, Haas A (2012) Secretive bacterial pathogens and the secretory pathway. Traffic. 13:1187\u20131197","journal-title":"Traffic."},{"key":"24_CR00248","doi-asserted-by":"publisher","first-page":"e17638","DOI":"10.1371\/journal.pone.0017638","volume":"6","author":"W Zhu","year":"2011","unstructured":"Zhu W, Banga S, Tan Y, Zheng C, Stephenson R, Gately J, Luo ZQ (2011) Comprehensive identification of protein substrates of the Dot\/Icm Type IV transporter of Legionella pneumophila. PLoS One 6:e17638","journal-title":"PLoS One"},{"key":"24_CR00249","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1146\/annurev-cellbio-100109-104034","volume":"26","author":"A Hubber","year":"2010","unstructured":"Hubber A, Roy CR (2010) Modulation of host cell function by Legionella pneumophila type IV effectors. Annu Rev Cell Dev Biol 26:261\u2013283","journal-title":"Annu Rev Cell Dev Biol"},{"key":"24_CR002410","doi-asserted-by":"crossref","first-page":"107","DOI":"10.4161\/cib.7713","volume":"2","author":"S Urwyler","year":"2009","unstructured":"Urwyler S, Brombacher E, Hilbi H (2009) Endosomal and secretory markers of the Legionella-containing vacuole. Commun Integr Biol 2:107\u2013109","journal-title":"Commun Integr Biol"},{"key":"24_CR002411","doi-asserted-by":"publisher","first-page":"1341","DOI":"10.1111\/j.1365-2958.2009.06608.x","volume":"71","author":"SS Weber","year":"2009","unstructured":"Weber SS, Ragaz C, Hilbi H (2009) Pathogen trafficking pathways and host phospho-inositide metabolism. Mol Microbiol 71: 1341\u20131352","journal-title":"Mol Microbiol"},{"key":"24_CR002412","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1083\/jcb.152.1.165","volume":"152","author":"J Garin","year":"2001","unstructured":"Garin J, Diez R, Kieffer S, Dermine JF, Duclos S, Gagnon E, Sadoul R, Rondeau C, Desjardins M (2001) The phagosome proteome: insight into phagosome functions. J Cell Biol 152: 165\u2013180","journal-title":"J Cell Biol"},{"key":"24_CR002413","first-page":"439","volume":"346","author":"D Gotthardt","year":"2006","unstructured":"Gotthardt D, Dieckmann R, Blancheteau V, Kistler C, Reichardt F, Soldati T (2006) Preparation of intact, highly purified phagosomes from Dictyostelium. Methods Mol Biol 346:439\u2013448","journal-title":"Methods Mol Biol"},{"key":"24_CR002414","doi-asserted-by":"publisher","first-page":"3508","DOI":"10.1091\/mbc.E02-04-0206","volume":"13","author":"D Gotthardt","year":"2002","unstructured":"Gotthardt D, Warnatz HJ, Henschel O, Bruckert F, Schleicher M, Soldati T (2002) High-resolution dissection of phagosome maturation reveals distinct membrane trafficking phases. Mol Biol Cell 13:3508\u20133520","journal-title":"Mol Biol Cell"},{"key":"24_CR002415","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.micpath.2004.11.003","volume":"38","author":"PE Kima","year":"2005","unstructured":"Kima PE, Dunn W (2005) Exploiting calnexin expression on phagosomes to isolate Leishmania parasitophorous vacuoles. Microb Pathog 38:139\u2013145","journal-title":"Microb Pathog"},{"key":"24_CR002416","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1023\/A:1017963401560","volume":"22","author":"A L\u00fchrmann","year":"2000","unstructured":"L\u00fchrmann A, Haas A (2000) A method to purify bacteria-containing phagosomes from infected macrophages. Methods Cell Sci 22:329\u2013341","journal-title":"Methods Cell Sci"},{"key":"24_CR002417","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1111\/j.1600-0854.2005.00304.x","volume":"6","author":"E Fernandez-Mora","year":"2005","unstructured":"Fernandez-Mora E, Polidori M, L\u00fchrmann A, Schaible UE, Haas A (2005) Maturation of Rhodococcus equi-containing vacuoles is arrested after completion of the early endosome stage. Traffic 6:635\u2013653","journal-title":"Traffic"},{"key":"24_CR002418","doi-asserted-by":"publisher","first-page":"2558","DOI":"10.1002\/elps.1150181411","volume":"18","author":"S Sturgill-Koszycki","year":"1997","unstructured":"Sturgill-Koszycki S, Haddix PL, Russell DG (1997) The interaction between Mycobacterium and the macrophage analyzed by two-dimensional polyacrylamide gel electrophoresis. Electrophoresis 18:2558\u20132565","journal-title":"Electrophoresis"},{"key":"24_CR002419","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/S0171-9335(98)80100-3","volume":"77","author":"SD Mills","year":"1998","unstructured":"Mills SD, Finlay BB (1998) Isolation and characterization of Salmonella typhimurium and Yersinia pseudotuberculosis-containing phagosomes from infected mouse macrophages: Y. pseudotuberculosis traffics to terminal lysosomes where they are degraded. Eur J Cell Biol 77:35\u201347","journal-title":"Eur J Cell Biol"},{"key":"24_CR002420","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1016\/j.ijmm.2009.03.006","volume":"299","author":"O Shevchuk","year":"2009","unstructured":"Shevchuk O, Batzilla C, H\u00e4gele S, Kusch H, Engelmann S, Hecker M, Haas A, Heuner K, Gl\u00f6ckner G, Steinert M (2009) Proteomic analysis of Legionella-containing phagosomes isolated from Dictyostelium. Int J Med Microbiol 299:489\u2013508","journal-title":"Int J Med Microbiol"},{"key":"24_CR002421","doi-asserted-by":"publisher","first-page":"e46","DOI":"10.1371\/journal.ppat.0020046","volume":"2","author":"SS Weber","year":"2006","unstructured":"Weber SS, Ragaz C, Reus K, Nyfeler Y, Hilbi H (2006) Legionella pneumophila exploits PI(4)P to anchor secreted effector proteins to the replicative vacuole. PLoS Pathog 2:e46","journal-title":"PLoS Pathog"},{"key":"24_CR002422","doi-asserted-by":"publisher","first-page":"2416","DOI":"10.1111\/j.1462-5822.2008.01219.x","volume":"10","author":"C Ragaz","year":"2008","unstructured":"Ragaz C, Pietsch H, Urwyler S, Tiaden A, Weber SS, Hilbi H (2008) The Legionella pneumophila phosphatidylinositol-4-phos-phate-binding type IV substrate SidC recruits endoplasmic reticulum vesicles to a replication-permissive vacuole. Cell Microbiol 10:2416\u20132433","journal-title":"Cell Microbiol"},{"key":"24_CR002423","doi-asserted-by":"publisher","first-page":"91","DOI":"10.3389\/fmicb.2011.00091","volume":"2","author":"H Hilbi","year":"2011","unstructured":"Hilbi H, Weber S, Finsel I (2011) Anchors for effectors: subversion of phosphoinositide lipids by Legionella. Front Microbiol 2:91","journal-title":"Front Microbiol"},{"key":"24_CR002424","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1111\/j.1600-0854.2008.00851.x","volume":"10","author":"S Urwyler","year":"2009","unstructured":"Urwyler S, Nyfeler Y, Ragaz C, Lee H, Mueller LN, Aebersold R, Hilbi H (2009) Proteome analysis of Legionella vacuoles purified by magnetic immunoseparation reveals secretory and endosomal GTPases. Traffic 10:76\u201387","journal-title":"Traffic"},{"key":"24_CR002425","unstructured":"Urwyler S, Finsel I, Ragaz C, Hilbi H (2010) Isolation of Legionella-containing vacuoles by immuno-magnetic separation. Curr Protoc Cell Biol. Chapter 3, Unit 3.34"},{"key":"24_CR002426","doi-asserted-by":"publisher","first-page":"6772","DOI":"10.1093\/emboj\/20.23.6772","volume":"20","author":"A M\u00fcller-Taubenberger","year":"2001","unstructured":"M\u00fcller-Taubenberger A, Lupas AN, Li H, Ecke M, Simmeth E, Gerisch G (2001) Calreticulin and calnexin in the endoplasmic reticulum are important for phagocytosis. EMBO J 20:6772\u20136782","journal-title":"EMBO J"},{"key":"24_CR002427","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1083\/jcb.45.2.399","volume":"45","author":"SM Cocucci","year":"1970","unstructured":"Cocucci SM, Sussman M (1970) RNA in cytoplasmic and nuclear fractions of cellular slime mold amebas. J Cell Biol 45:399\u2013407","journal-title":"J Cell Biol"},{"key":"24_CR002428","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1111\/j.1432-1033.1972.tb01894.x","volume":"28","author":"D Malchow","year":"1972","unstructured":"Malchow D, N\u00e4gele B, Schwarz H, Gerisch G (1972) Membrane-bound cyclic AMP phosphodiesterase in chemotactically responding cells of Dictyostelium discoideum. Eur J Biochem 28:136\u2013142","journal-title":"Eur J Biochem"},{"key":"24_CR002429","doi-asserted-by":"publisher","first-page":"2885","DOI":"10.1128\/AEM.72.4.2885-2895.2006","volume":"72","author":"J Mampel","year":"2006","unstructured":"Mampel J, Spirig T, Weber SS, Haagensen JAJ, Molin S, Hilbi H (2006) Planktonic replication is essential for biofilm formation by Legionella pneumophila in a complex medium under static and dynamic flow conditions. Appl Environ Microbiol 72:2885\u20132895","journal-title":"Appl Environ Microbiol"},{"key":"24_CR002430","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.1084\/jem.158.4.1319","volume":"158","author":"MA Horwitz","year":"1983","unstructured":"Horwitz MA (1983) Formation of a novel phagosome by the Legionnaires\u2019 disease bacterium (Legionella pneumophila) in human monocytes. J Exp Med 158:1319\u20131331","journal-title":"J Exp Med"},{"key":"24_CR002431","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1128\/jcm.10.4.437-441.1979","volume":"10","author":"JC Feeley","year":"1979","unstructured":"Feeley JC, Gibson RJ, Gorman GW, Langford NC, Rasheed JK, Mackel DC, Baine WB (1979) Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila. J Clin Microbiol 10:437\u2013441","journal-title":"J Clin Microbiol"},{"key":"24_CR002432","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1111\/j.1462-5822.2005.00528.x","volume":"7","author":"H Lu","year":"2005","unstructured":"Lu H, Clarke M (2005) Dynamic properties of Legionella-containing phagosomes in Dictyostelium amoebae. Cell Microbiol 7: 995\u20131007","journal-title":"Cell Microbiol"}],"container-title":["Methods in Molecular Biology","Dictyostelium discoideum Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-62703-302-2_24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,19]],"date-time":"2023-02-19T10:21:57Z","timestamp":1676802117000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-62703-302-2_24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"ISBN":["9781627033015","9781627033022"],"references-count":32,"URL":"https:\/\/doi.org\/10.1007\/978-1-62703-302-2_24","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"type":"print","value":"1064-3745"},{"type":"electronic","value":"1940-6029"}],"subject":[],"published":{"date-parts":[[2013]]},"assertion":[{"value":"8 February 2013","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}