{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T06:51:01Z","timestamp":1744181461604,"version":"3.40.3"},"publisher-location":"Cham","reference-count":38,"publisher":"Springer Nature Switzerland","isbn-type":[{"type":"print","value":"9783031395031"},{"type":"electronic","value":"9783031395048"}],"license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"vor","delay-in-days":212,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Many aquatic habitats have become vulnerable to rapid and long-term changes induced by industrialism, air pollution, tourism, fishing activities etc. These factors created an urgent need for extensive water monitoring and conservation. By observing the behaviour of lifeforms, we can monitor the state of the environment. Here, we present the methodology, calibration approaches and preliminary results of designing a biohybrid entity for aquatic monitoring. Biohybrid robots combine mechanical and electronic elements with living organisms or tissues. This biohybrid consists of several modules, each hosting or attracting different species and communities. We focus on animals such as <jats:italic>Daphnia<\/jats:italic> sp., zebra mussel <jats:italic>Dreissena polymorpha<\/jats:italic> and various representatives of the plankton community. The first results showed that 1) both <jats:italic>Daphnia<\/jats:italic> and <jats:italic>D. polymorpha<\/jats:italic> show no clear signs of confinement-induced stress, 2) the designed structures are examples of suitable tools for hosting the organisms, observing their behaviour and collecting and storing data and 3) their behaviour can be calibrated under laboratory conditions to be able to extrapolate the field data into environmental data.<\/jats:p>","DOI":"10.1007\/978-3-031-39504-8_24","type":"book-chapter","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T08:02:32Z","timestamp":1690790552000},"page":"348-362","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Living Organisms as\u00a0Sensors for\u00a0Biohybrid Monitoring Systems"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6328-2023","authenticated-orcid":false,"given":"Wiktoria","family":"Rajewicz","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8064-2155","authenticated-orcid":false,"given":"Nikolaus","family":"Helmer","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8598-7462","authenticated-orcid":false,"given":"Thomas","family":"Schmickl","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0420-4444","authenticated-orcid":false,"given":"Ronald","family":"Thenius","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,8,1]]},"reference":[{"issue":"2","key":"24_CR1","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1016\/j.cbpb.2003.11.003","volume":"137","author":"JE Alexander Jr","year":"2004","unstructured":"Alexander, J.E., Jr., McMahon, R.F.: Respiratory response to temperature and hypoxia in the zebra mussel Dreissena polymorpha. Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol. 137(2), 425\u2013434 (2004)","journal-title":"Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol."},{"key":"24_CR2","unstructured":"AquaDect: Mosselmonitor\u00ae. https:\/\/aquadect.nl\/en\/. Accessed 28 Mar 2023"},{"key":"24_CR3","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1016\/j.scitotenv.2013.07.075","volume":"466\u2013467","author":"MJ Bae","year":"2014","unstructured":"Bae, M.J., Park, Y.S.: Biological early warning system based on the responses of aquatic organisms to disturbances: a review. Sci. Total Environ. 466\u2013467, 635\u2013649 (2014). https:\/\/doi.org\/10.1016\/j.scitotenv.2013.07.075","journal-title":"Sci. Total Environ."},{"key":"24_CR4","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1016\/j.scitotenv.2017.05.199","volume":"601","author":"A Bownik","year":"2017","unstructured":"Bownik, A.: Daphnia swimming behaviour as a biomarker in toxicity assessment: a review. Sci. Total Environ. 601, 194\u2013205 (2017)","journal-title":"Sci. Total Environ."},{"issue":"3","key":"24_CR5","first-page":"259","volume":"34","author":"LC Clark Jr","year":"1988","unstructured":"Clark, L.C., Jr., Spokane, R.B., Homan, M.M., Sudan, R., Miller, M.: Long-term stability of electroenzymatic glucose sensors implanted in mice. An update. ASAIO Trans. 34(3), 259\u2013265 (1988)","journal-title":"ASAIO Trans."},{"issue":"2","key":"24_CR6","doi-asserted-by":"publisher","first-page":"126","DOI":"10.4319\/lom.2008.6.126","volume":"6","author":"RK Cowen","year":"2008","unstructured":"Cowen, R.K., Guigand, C.M.: In situ ichthyoplankton imaging system (ISIIS): system design and preliminary results. Limnol. Oceanogr. Methods 6(2), 126\u2013132 (2008). https:\/\/doi.org\/10.4319\/lom.2008.6.126","journal-title":"Limnol. Oceanogr. Methods"},{"key":"24_CR7","doi-asserted-by":"publisher","unstructured":"Damania, R., Desbureaux, S., Rodella, A.S., Russ, J., Zaveri, E.: Quality Unknown: the Invisible Water Crisis. World Bank Publications (2019). https:\/\/doi.org\/10.1596\/978-1-4648-1459-4","DOI":"10.1596\/978-1-4648-1459-4"},{"key":"24_CR8","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1007\/s10452-009-9282-3","volume":"43","author":"MT Dokulil","year":"2009","unstructured":"Dokulil, M.T., Herzig, A.: An analysis of long-term winter data on phytoplankton and zooplankton in Neusiedler See, a shallow temperate lake, Austria. Aquat. Ecol. 43, 715\u2013725 (2009)","journal-title":"Aquat. Ecol."},{"key":"24_CR9","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/s10750-019-04007-0","volume":"841","author":"A Dzier\u017cy\u0144ska-Bia\u0142o\u0144czyk","year":"2019","unstructured":"Dzier\u017cy\u0144ska-Bia\u0142o\u0144czyk, A., Jermacz, \u0141, Zielska, J., Kobak, J.: What scares a mussel? Changes in valve movement pattern as an immediate response of a byssate bivalve to biotic factors. Hydrobiologia 841, 65\u201377 (2019)","journal-title":"Hydrobiologia"},{"key":"24_CR10","unstructured":"Ebert, D.: Ecology, epidemiology, and evolution of parasitism in Daphnia. National Library of Medicine (2005)"},{"key":"24_CR11","unstructured":"Enviro-Analytical: Daphnia Toximeter II. A powerful instrument for water toxicity assessment- continuous visual analysis of Daphnia behaviour (2021). http:\/\/www.enviro-analytical.com\/preview\/enviroproducts\/daphnia_toximeter.html. Accessed 25 Feb 2021"},{"key":"24_CR12","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.actbio.2020.12.002","volume":"121","author":"L Gao","year":"2021","unstructured":"Gao, L., et al.: Recent progress in engineering functional biohybrid robots actuated by living cells. Acta Biomater. 121, 29\u201340 (2021)","journal-title":"Acta Biomater."},{"issue":"6","key":"24_CR13","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1002\/tox.10138","volume":"18","author":"U Green","year":"2003","unstructured":"Green, U., Kremer, J.H., Zillmer, M., Moldaenke, C.: Detection of chemical threat agents in drinking water by an early warning real-time biomonitor. Environ. Toxicol. 18(6), 368\u2013374 (2003). https:\/\/doi.org\/10.1002\/tox.10138","journal-title":"Environ. Toxicol."},{"issue":"1","key":"24_CR14","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1007\/s10750-011-0831-0","volume":"676","author":"E Jeppesen","year":"2011","unstructured":"Jeppesen, E., N\u00f5ges, P., Davidson, T.A., Haberman, J., N\u00f5ges, T., Blank, K., et al.: Zooplankton as indicators in lakes: a scientific-based plea for including zooplankton in the ecological quality assessment of lakes according to the European Water Framework Directive (WFD). Hydrobiologia 676(1), 279\u2013297 (2011). https:\/\/doi.org\/10.1007\/s10750-011-0831-0","journal-title":"Hydrobiologia"},{"issue":"6581","key":"24_CR15","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1126\/science.abh0474","volume":"375","author":"KY Lee","year":"2022","unstructured":"Lee, K.Y., et al.: An autonomously swimming biohybrid fish designed with human cardiac biophysics. Science 375(6581), 639\u2013647 (2022)","journal-title":"Science"},{"key":"24_CR16","doi-asserted-by":"publisher","first-page":"9891","DOI":"10.1007\/s11356-019-07592-8","volume":"27","author":"L Lomba","year":"2020","unstructured":"Lomba, L., et al.: Ecotoxicological study of six drugs in Aliivibrio fischeri, Daphnia magna and Raphidocelis subcapitata. Environ. Sci. Pollut. Res. 27, 9891\u20139900 (2020). https:\/\/doi.org\/10.1007\/s11356-019-07592-8","journal-title":"Environ. Sci. Pollut. Res."},{"key":"24_CR17","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1111\/brv.12244","volume":"92","author":"M Lopes-Lima","year":"2017","unstructured":"Lopes-Lima, M., Sousa, R., Geist, J., Aldridge, D.C., Araujo, R., Jakob, B., et al.: Conservation status of freshwater mussels in Europe: state of the art and future challenges. Biol. Rev. 92, 572\u2013607 (2017). https:\/\/doi.org\/10.1111\/brv.12244","journal-title":"Biol. Rev."},{"issue":"44","key":"24_CR18","doi-asserted-by":"publisher","first-page":"66100","DOI":"10.1007\/s11356-022-20203-3","volume":"29","author":"Y Ma","year":"2022","unstructured":"Ma, Y., et al.: Combined toxicity and toxicity persistence of antidepressants citalopram and mirtazapine to zooplankton Daphnia magna. Environ. Sci. Pollut. Res. 29(44), 66100\u201366108 (2022)","journal-title":"Environ. Sci. Pollut. Res."},{"issue":"3","key":"24_CR19","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/j.ecoenv.2006.11.003","volume":"67","author":"J Martins","year":"2007","unstructured":"Martins, J., Soares, M., Saker, M.L., Oliva-Teles, L., Vasconcelos, V.: Phototactic behavior in Daphnia magna Straus as an indicator of toxicants in the aquatic environment. Ecotoxicol. Environ. Saf. 67(3), 417\u2013422 (2007)","journal-title":"Ecotoxicol. Environ. Saf."},{"issue":"5","key":"24_CR20","volume":"13","author":"R Mestre","year":"2021","unstructured":"Mestre, R., Pati\u00f1o, T., S\u00e1nchez, S.: Biohybrid robotics: from the nanoscale to the macroscale. Wiley Interdisc. Rev.: Nanomed. Nanobiotechnol. 13(5), e1703 (2021)","journal-title":"Wiley Interdisc. Rev.: Nanomed. Nanobiotechnol."},{"key":"24_CR21","doi-asserted-by":"publisher","unstructured":"Morimoto, Y., Onoe, H., Takeuchi, S.: Biohybrid robot powered by an antagonistic pair of skeletal muscle tissues. Sci. Robot. 3(18), eaat4440 (2018). https:\/\/doi.org\/10.1126\/scirobotics.aat4440","DOI":"10.1126\/scirobotics.aat4440"},{"issue":"13","key":"24_CR22","first-page":"74","volume":"8","author":"O Nikitin","year":"2014","unstructured":"Nikitin, O.: Aqueous medium toxicity assessment by Daphnia magna swimming activity change. Adv. Environ. Biol. 8(13), 74\u201378 (2014)","journal-title":"Adv. Environ. Biol."},{"issue":"6295","key":"24_CR23","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1126\/science.aaf4292","volume":"353","author":"SJ Park","year":"2016","unstructured":"Park, S.J., et al.: Phototactic guidance of a tissue-engineered soft-robotic ray. Science 353(6295), 158\u2013162 (2016)","journal-title":"Science"},{"key":"24_CR24","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1007\/s00422-021-00902-9","volume":"115","author":"W Rajewicz","year":"2021","unstructured":"Rajewicz, W., et al.: Freshwater organisms potentially useful as biosensors and power-generation mediators in biohybrid robotics. Biol. Cybern. 115, 615\u2013628 (2021)","journal-title":"Biol. Cybern."},{"key":"24_CR25","series-title":"Mechanisms and Machine Science","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1007\/978-3-031-04870-8_61","volume-title":"Advances in Service and Industrial Robotics","author":"W Rajewicz","year":"2022","unstructured":"Rajewicz, W., Schmickl, T., Thenius, R.: The use of robots in aquatic biomonitoring with special focus on biohybrid entities. In: M\u00fcller, A., Brandst\u00f6tter, M. (eds.) RAAD 2022. Mechanisms and Machine Science, vol. 120, pp. 521\u2013527. Springer, Cham (2022). https:\/\/doi.org\/10.1007\/978-3-031-04870-8_61"},{"key":"24_CR26","unstructured":"Reynolds, C.S., O\u2019Sullivan, P.E.: The Lakes Handbook: Lake Restoration and Rehabilitation. Blackwell Publishing (2003)"},{"key":"24_CR27","doi-asserted-by":"publisher","unstructured":"Ricotti, L., et al.: Biohybrid actuators for robotics: a review of devices actuated by living cells. Sci. Robot. 2(12), eaaq0495 (2017). https:\/\/doi.org\/10.1126\/scirobotics.aaq0495","DOI":"10.1126\/scirobotics.aaq0495"},{"key":"24_CR28","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1007\/s00422-018-0787-5","volume":"113","author":"D Romano","year":"2019","unstructured":"Romano, D., Donati, E., Benelli, G., Stefanini, C.: A review on animal-robot interaction: from bio-hybrid organisms to mixed societies. Biol. Cybern. 113, 201\u2013225 (2019)","journal-title":"Biol. Cybern."},{"issue":"2","key":"24_CR29","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.cvsm.2008.10.010","volume":"39","author":"PL Schmidt","year":"2009","unstructured":"Schmidt, P.L.: Companion animals as sentinels for public health. Vet. Clin. North America: Small Animal Pract. 39(2), 241\u2013250 (2009)","journal-title":"Vet. Clin. North America: Small Animal Pract."},{"issue":"1","key":"24_CR30","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.forsciint.2004.04.080","volume":"147","author":"G Schoon","year":"2005","unstructured":"Schoon, G.: The effect of the ageing of crime scene objects on the results of scent identification line-ups using trained dogs. Forensic Sci. Int. 147(1), 43\u201347 (2005)","journal-title":"Forensic Sci. Int."},{"key":"24_CR31","doi-asserted-by":"publisher","first-page":"38792","DOI":"10.1007\/s11356-021-13200-5","volume":"28","author":"AP Sousa","year":"2021","unstructured":"Sousa, A.P., Nunes, B.: Dangerous connections: biochemical and behavioral traits in Daphnia magna and Daphnia longispina exposed to ecologically relevant amounts of paracetamol. Environ. Sci. Pollut. Res. 28, 38792\u201338808 (2021)","journal-title":"Environ. Sci. Pollut. Res."},{"key":"24_CR32","first-page":"231","volume":"5","author":"T Spencer","year":"1962","unstructured":"Spencer, T.: Effects of carbon monoxide on man and canaries. Ann. Occup. Hyg. 5, 231\u2013234 (1962)","journal-title":"Ann. Occup. Hyg."},{"issue":"4","key":"24_CR33","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1016\/j.tube.2011.04.008","volume":"91","author":"DM Suckling","year":"2011","unstructured":"Suckling, D.M., Sagar, R.L.: Honeybees Apis mellifera can detect the scent of Mycobacterium tuberculosis. Tuberculosis 91(4), 327\u2013328 (2011)","journal-title":"Tuberculosis"},{"key":"24_CR34","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.128660","volume":"270","author":"A Szabelak","year":"2021","unstructured":"Szabelak, A., Bownik, A.: Behavioral and physiological responses of Daphnia magna to salicylic acid. Chemosphere 270, 128660 (2021)","journal-title":"Chemosphere"},{"issue":"03","key":"24_CR35","doi-asserted-by":"publisher","first-page":"341","DOI":"10.4236\/ojms.2016.63029","volume":"6","author":"B Tang","year":"2016","unstructured":"Tang, B., Riisg\u00e5rd, H.U., et al.: Physiological regulation of valve-opening degree enables mussels Mytilus edulis to overcome starvation periods by reducing the oxygen uptake. Open J. Mar. Sci. 6(03), 341 (2016)","journal-title":"Open J. Mar. Sci."},{"key":"24_CR36","doi-asserted-by":"crossref","unstructured":"Thenius, R., et al.: Biohybrid entities for environmental monitoring. In: ALIFE 2022: The 2022 Conference on Artificial Life. MIT Press (2021)","DOI":"10.1162\/isal_a_00366"},{"key":"24_CR37","doi-asserted-by":"crossref","unstructured":"Webster-Wood, V.A., et al.: Biohybrid robots: recent progress, challenges, and perspectives. Bioinspiration Biomimetics (2022)","DOI":"10.1088\/1748-3190\/ac9c3b"},{"key":"24_CR38","first-page":"1","volume":"79","author":"G Wieser","year":"2013","unstructured":"Wieser, G., Schulz, L.: Alpine Lakes Network SILMAS Beitrag K\u00e4rnten-Endbericht. Publikationen des K\u00e4rntner Instituts f\u00fcr Seenforschung 79, 1\u201344 (2013)","journal-title":"Publikationen des K\u00e4rntner Instituts f\u00fcr Seenforschung"}],"container-title":["Lecture Notes in Computer Science","Biomimetic and Biohybrid Systems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-3-031-39504-8_24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T07:04:37Z","timestamp":1700809477000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-3-031-39504-8_24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"ISBN":["9783031395031","9783031395048"],"references-count":38,"URL":"https:\/\/doi.org\/10.1007\/978-3-031-39504-8_24","relation":{},"ISSN":["0302-9743","1611-3349"],"issn-type":[{"type":"print","value":"0302-9743"},{"type":"electronic","value":"1611-3349"}],"subject":[],"published":{"date-parts":[[2023]]},"assertion":[{"value":"1 August 2023","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"Living Machines","order":1,"name":"conference_acronym","label":"Conference Acronym","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"Conference on Biomimetic and Biohybrid Systems","order":2,"name":"conference_name","label":"Conference Name","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"Genoa","order":3,"name":"conference_city","label":"Conference City","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"Italy","order":4,"name":"conference_country","label":"Conference Country","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"2023","order":5,"name":"conference_year","label":"Conference Year","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"10 July 2023","order":7,"name":"conference_start_date","label":"Conference Start Date","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"13 July 2023","order":8,"name":"conference_end_date","label":"Conference End Date","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"12","order":9,"name":"conference_number","label":"Conference Number","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"lm2023","order":10,"name":"conference_id","label":"Conference ID","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"https:\/\/livingmachinesconference.eu\/2023\/","order":11,"name":"conference_url","label":"Conference URL","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"Single-blind","order":1,"name":"type","label":"Type","group":{"name":"ConfEventPeerReviewInformation","label":"Peer Review Information (provided by the conference organizers)"}},{"value":"EquinOCS","order":2,"name":"conference_management_system","label":"Conference Management System","group":{"name":"ConfEventPeerReviewInformation","label":"Peer Review Information (provided by the conference organizers)"}},{"value":"67","order":3,"name":"number_of_submissions_sent_for_review","label":"Number of Submissions Sent for Review","group":{"name":"ConfEventPeerReviewInformation","label":"Peer Review Information (provided by the conference organizers)"}},{"value":"44","order":4,"name":"number_of_full_papers_accepted","label":"Number of Full Papers Accepted","group":{"name":"ConfEventPeerReviewInformation","label":"Peer Review Information (provided by the conference organizers)"}},{"value":"14","order":5,"name":"number_of_short_papers_accepted","label":"Number of Short Papers Accepted","group":{"name":"ConfEventPeerReviewInformation","label":"Peer Review Information (provided by the conference organizers)"}},{"value":"66% - The value is computed by the equation \"Number of Full Papers Accepted \/ Number of Submissions Sent for Review * 100\" and then rounded to a whole number.","order":6,"name":"acceptance_rate_of_full_papers","label":"Acceptance Rate of Full Papers","group":{"name":"ConfEventPeerReviewInformation","label":"Peer Review Information (provided by the conference organizers)"}},{"value":"2.7","order":7,"name":"average_number_of_reviews_per_paper","label":"Average Number of Reviews per Paper","group":{"name":"ConfEventPeerReviewInformation","label":"Peer Review Information (provided by the conference organizers)"}},{"value":"1.1","order":8,"name":"average_number_of_papers_per_reviewer","label":"Average Number of Papers per Reviewer","group":{"name":"ConfEventPeerReviewInformation","label":"Peer Review Information (provided by the conference organizers)"}},{"value":"Yes","order":9,"name":"external_reviewers_involved","label":"External Reviewers Involved","group":{"name":"ConfEventPeerReviewInformation","label":"Peer Review Information (provided by the conference organizers)"}}]}}