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Accurate diagnosis of Mpox can be challenging, but history, clinical findings, and laboratory diagnosis can establish the diagnosis. The pre-analytic phase of testing includes collecting, storing, and transporting specimens. It is advised to swab the lesion site with virus transport medium (VTM) containing Dacron or polyester flock swabs from two different sites. Blood, urine, and semen samples may also be used. Timely sampling is necessary to obtain a sufficient amount of virus or antibodies. The analytical phase of infectious disease control involves diagnostic tools to determine the presence of the virus. While polymerase chain reaction (PCR) is the gold standard for detecting Mpox, genome sequencing is for identifying new or modified viruses. As a complement to these methods, isothermal amplification methods have been designed. ELISAassays are also available for the determination of antibodies. Electron microscopy is another effective diagnostic method for tissue identification of the virus. Wastewater fingerprinting provides some of the most effective diagnostic methods for virus identification at the community level. The advantages and disadvantages of these methods are further discussed. Post-analytic phase requires proper interpretation of test results and the preparation of accurate patient reports that include relevant medical history, clinical guidelines, and recommendations for follow-up testing or treatment.<\/jats:p>","DOI":"10.1007\/978-3-031-57165-7_16","type":"book-chapter","created":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T07:02:03Z","timestamp":1716793323000},"page":"253-271","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Monkeypox Diagnosis in Clinical Settings: A Comprehensive Review of Best Laboratory Practices"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7918-7909","authenticated-orcid":false,"given":"Nityanand","family":"Jain","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6975-8096","authenticated-orcid":false,"given":"Tungki Pratama","family":"Umar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4378-2714","authenticated-orcid":false,"given":"Reem","family":"Sayad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0239-5814","authenticated-orcid":false,"given":"Muhammed Edib","family":"Mokresh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6998-8091","authenticated-orcid":false,"given":"Kevin","family":"Tandarto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0317-4113","authenticated-orcid":false,"given":"Reynold","family":"Siburian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2081-180X","authenticated-orcid":false,"given":"Phey","family":"Liana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2325-6151","authenticated-orcid":false,"given":"Sniedze","family":"Laivacuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3671-1213","authenticated-orcid":false,"given":"Aigars","family":"Reinis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,28]]},"reference":[{"issue":"3","key":"16_CR1","first-page":"82","volume":"5","author":"OA Adegoke","year":"2011","unstructured":"Adegoke OA, Idowu AA, Jeje OA (2011) Incomplete laboratory request forms as a contributory factor to preanalytical errors in a Nigerian teaching hospital. 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