{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T04:38:07Z","timestamp":1747802287078,"version":"3.41.0"},"publisher-location":"Cham","reference-count":32,"publisher":"Springer International Publishing","isbn-type":[{"type":"print","value":"9783319157047"},{"type":"electronic","value":"9783319157054"}],"license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015]]},"DOI":"10.1007\/978-3-319-15705-4_29","type":"book-chapter","created":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T05:21:23Z","timestamp":1426483283000},"page":"297-306","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Automated Analysis of Images from Confocal Laser Scanning Microscopy Applied to Observation of Calcium Channel Subunits in Nerve Cell Model Line Subjected Electroporation and Calcium"],"prefix":"10.1007","author":[{"given":"Julita","family":"Kulbacka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Kulbacki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakub","family":"Segen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Choroma\u0144ska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jolanta","family":"Saczko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magda","family":"Dubi\u0144ska-Magiera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma\u0142gorzata","family":"Kotulska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,3,17]]},"reference":[{"key":"29_CR1","doi-asserted-by":"crossref","unstructured":"Kanade, T., et al.: Cell image analysis: Algorithms, system and applications. In: 2011 IEEE Workshop on Applications of Computer Vision (WACV), IEEE (2011)","DOI":"10.1109\/WACV.2011.5711528"},{"issue":"1","key":"29_CR2","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1002\/cyto.10026","volume":"47","author":"C W\u00e4hlby","year":"2002","unstructured":"W\u00e4hlby, C., Erlandsson, F., Bengtsson, E., Zetterberg, A.: Sequential immunofluorescence staining and image analysis for detection of large numbers of antigens in individual cell nuclei. Cytometry 47(1), 32\u201341 (2002)","journal-title":"Cytometry"},{"key":"29_CR3","series-title":"Lecture Notes in Computer Science","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1007\/978-3-319-10404-1_6","volume-title":"Medical Image Computing and Computer-Assisted Intervention \u2013 MICCAI 2014","author":"Z Yin","year":"2014","unstructured":"Yin, Z., Su, H., Ker, E., Li, M., Li, H.: Cell-Sensitive Microscopy Imaging for Cell Image Segmentation. In: Golland, P., Hata, N., Barillot, C., Hornegger, J., Howe, R. (eds.) MICCAI 2014, Part I. LNCS, vol. 8673, pp. 41\u201348. Springer, Heidelberg (2014)"},{"issue":"23","key":"29_CR4","doi-asserted-by":"crossref","first-page":"7557","DOI":"10.1523\/JNEUROSCI.16-23-07557.1996","volume":"16","author":"H Liu","year":"1996","unstructured":"Liu, H., Felix, R., Gurnett, C., De Waard, M., Witcher, D.R., Campbell, K.P.: Expression and Subunit Interaction of Voltage-Dependent Ca2+ Channels in PC12 Cells. The Journal of Neuroscience 16(23), 7557\u20137565 (1996)","journal-title":"The Journal of Neuroscience"},{"key":"29_CR5","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1038\/360169a0","volume":"360","author":"KG Beam","year":"1992","unstructured":"Beam, K.G., Adams, B.A., Niidome, T., Numa, S.: Tanabe T Function of a truncated dihydropyridine receptor as both voltage sensor and calcium channel. Nature 360, 169\u2013171 (1992)","journal-title":"Nature"},{"key":"29_CR6","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1002\/neu.480250309","volume":"25","author":"A Ghosh","year":"1994","unstructured":"Ghosh, A., Ginty, D.D., Bading, H., Greenberg, M.E.: Calcium regulation of gene expression in neuronal cells. J. Neurobiol. 25, 294\u2013303 (1994)","journal-title":"J. Neurobiol."},{"key":"29_CR7","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/0166-2236(95)93882-X","volume":"18","author":"K Dunlap","year":"1995","unstructured":"Dunlap, K.: Luebke JI, Turner TJ Exocytotic Ca2+ channels in mammalian central neurons. Trends. Neurosci. 18, 89\u201396 (1995)","journal-title":"Trends. Neurosci."},{"issue":"7","key":"29_CR8","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., Tinevez, J.Y., White, D.J., Hartenstein, V., Eliceiri, K., Tomancak, P., Cardona, A.: Fiji: an open-source platform for biological-image analysis. Nature Methods 9(7), 676\u2013682 (2012). doi:10.1038\/nmeth.2019","journal-title":"Nature Methods"},{"key":"29_CR9","unstructured":"R Development Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL (2008). http:\/\/www.R-project.org"},{"issue":"1","key":"29_CR10","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/0031-3203(94)E0043-K","volume":"28","author":"L-K Huang","year":"1995","unstructured":"Huang, L.-K., Wang, M.-J.J.: Image thresholding by minimizing the measure of fuzziness. Pattern Recognition 28(1), 41\u201351 (1995)","journal-title":"Pattern Recognition"},{"key":"29_CR11","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1111\/j.1749-6632.1965.tb11715.x","volume":"128","author":"JMS Prewitt","year":"1966","unstructured":"Prewitt, J.M.S., Mendelsohn, M.L.: The analysis of cell images. Annals of the New York Academy of Sciences 128, 1035\u20131053 (1966)","journal-title":"Annals of the New York Academy of Sciences"},{"issue":"4","key":"29_CR12","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1016\/0031-3203(93)90115-D","volume":"26","author":"CH Li","year":"1993","unstructured":"Li, C.H., Lee, C.K.: Minimum Cross Entropy Thresholding. Pattern Recognition 26(4), 617\u2013625 (1993)","journal-title":"Pattern Recognition"},{"issue":"3","key":"29_CR13","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/0734-189X(85)90125-2","volume":"29","author":"JN Kapur","year":"1985","unstructured":"Kapur, J.N., Sahoo, P.K., Wong, A.C.K.: A New Method for Gray-Level Picture Thresholding Using the Entropy of the Histogram. Graphical Models and Image Processing 29(3), 273\u2013285 (1985)","journal-title":"Graphical Models and Image Processing"},{"key":"29_CR14","doi-asserted-by":"crossref","unstructured":"Kittler, J., Illingworth, J.: Minimum error thresholding. Pattern Recognition 19 (1986)","DOI":"10.1016\/0031-3203(86)90030-0"},{"key":"29_CR15","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1016\/0734-189X(85)90133-1","volume":"29","author":"W Tsai","year":"1985","unstructured":"Tsai, W.: Moment-preserving thresholding: a new approach. Computer Vision, Graphics, and Image Processing 29, 377\u2013393 (1985)","journal-title":"Computer Vision, Graphics, and Image Processing"},{"key":"29_CR16","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1109\/TSMC.1979.4310076","volume":"9","author":"N Otsu","year":"1979","unstructured":"Otsu, N.: A threshold selection method from gray-level histograms. IEEE Trans. Sys., Man Cyber. 9, 62\u201366 (1979). doi:10.1109\/TSMC.1979.4310076","journal-title":"IEEE Trans. Sys., Man Cyber."},{"key":"29_CR17","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1145\/321119.321123","volume":"9","author":"W Doyle","year":"1962","unstructured":"Doyle, W.: Operation useful for similarity-invariant pattern recognition. Journal of the Association for Computing Machinery 9, 259\u2013267 (1962). doi:10.1145\/321119.321123","journal-title":"Journal of the Association for Computing Machinery"},{"key":"29_CR18","doi-asserted-by":"crossref","unstructured":"Shanbhag, A.G.: Utilization of information measure as a means of image thresholding. Graph. Models Image Process. (Academic Press, Inc.) 56(5), 414\u2013419, ISSN 1049-9652 (1994)","DOI":"10.1006\/cgip.1994.1037"},{"key":"29_CR19","doi-asserted-by":"crossref","unstructured":"Zack, G.W., Rogers, W.E., Latt, S.A.: Automatic measurement of sister chromatid exchange frequency. J. Histochem. Cytochem 25(7), 741\u201353. PMID 70454 (1977)","DOI":"10.1177\/25.7.70454"},{"key":"29_CR20","doi-asserted-by":"publisher","unstructured":"Yen, J.C., Chang, F.J., Chang, S.: A New Criterion for Automatic Multilevel Thresholding. IEEE Trans. on Image Processing 4(3), 370\u2013378, ISSN 1057-7149, (1995). doi:10.1109\/83.366472","DOI":"10.1109\/83.366472"},{"key":"29_CR21","doi-asserted-by":"publisher","first-page":"630","DOI":"10.1109\/TSMC.1978.4310039","volume":"8","author":"TW Ridler","year":"1978","unstructured":"Ridler, T.W., Calvard, S.: Picture thresholding using an iterative selection method. IEEE Transactions on Systems, Man and Cybernetics 8, 630\u2013632 (1978)","journal-title":"IEEE Transactions on Systems, Man and Cybernetics"},{"key":"29_CR22","doi-asserted-by":"publisher","unstructured":"Henriques, R., Lelek, M., Fornasiero, E.F., Valtorta, F.: Christophe Zimmer & Musa M Mhlanga. QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ. Nature Methods 7, 339\u2013340. (2010). doi:10.1038\/nmeth0510-339","DOI":"10.1038\/nmeth0510-339"},{"key":"29_CR23","volume-title":"Information Theory and Statistics","author":"S Kullback","year":"1968","unstructured":"Kullback, S.: Information Theory and Statistics. Dover, New York (1968)"},{"key":"29_CR24","unstructured":"Puzicha, J., Hofmann, T., Buhmann, J.M.: Non-parametric similarity measures for unsupervised texture segmentation and image retrieval. In: Proceedings of CVPR (June 1997)"},{"key":"29_CR25","doi-asserted-by":"crossref","unstructured":"Rubner, Y., Tomasi, C., Guibas, L.J.: A metric for distributions with applications to image databases. In: IEEE International Conference on Computer Vision, pp. 59\u201366 (January 1998)","DOI":"10.1109\/ICCV.1998.710701"},{"key":"29_CR26","doi-asserted-by":"publisher","first-page":"4171","DOI":"10.1046\/j.1460-9568.1999.00849.x","volume":"11","author":"U Klockner","year":"1999","unstructured":"Klockner, U., Lee, J.H., Cribbs, L.L., Daud, A., Hescheler, J., Pereverzev, A., Perez-Reyes, E., Schneider, T.: Comparison of the Ca2+ currents induced by expression of three cloned \u03b11 subunits, \u03b11G, \u03b11H and \u03b11I, of low-voltage-activated T-type Ca2+ channels. Eur J Neurosci 11, 4171\u20134178 (1999)","journal-title":"Eur J Neurosci"},{"issue":"3","key":"29_CR27","doi-asserted-by":"publisher","first-page":"271","DOI":"10.2174\/1389200211314030001","volume":"14","author":"L Tang","year":"2013","unstructured":"Tang, L.: Electroporation for drug and gene delivery in cancer therapy. Curr. Drug. Metab. 14(3), 271 (2013)","journal-title":"Curr. Drug. Metab."},{"key":"29_CR28","doi-asserted-by":"crossref","unstructured":"Gibot, L., Wasungu, L., Teissi\u00e9, J., Rols, M.P.: Antitumor drug delivery in multicellular spheroids by electropermeabilization. J. Control Release 28, 167(2), 138\u201347 (2013)","DOI":"10.1016\/j.jconrel.2013.01.021"},{"key":"29_CR29","unstructured":"Kulbacka, J., Daczewska, M., Dubi\u0144ska-Magiera, M., Choroma\u0144ska, A., Rembia\u0142kowska, N., Surowiak, P., Kulbacki, M., Kotulska, M., Saczko, J.: Doxorubicin delivery enhanced by electroporation to gastrointestinal adenocarcinoma cells with P-gp overexpression. Bioelectrochemistry. pii: S1567\u20135394(14) 00065-6 (April 4, 2014)"},{"issue":"6","key":"29_CR30","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1007\/s12192-013-0423-1","volume":"18","author":"J Kulbacka","year":"2013","unstructured":"Kulbacka, J., Kotulska, M., Rembia\u0142kowska, N., Choroma\u0144ska, A., Kami\u0144ska, I., Garbiec, A., Rossowska, J., Daczewska, M., Jachimska, B., Saczko, J.: Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX). Cell Stress Chaperones 18(6), 719\u2013731 (2013)","journal-title":"Cell Stress Chaperones"},{"issue":"5","key":"29_CR31","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1002\/bem.21696","volume":"33","author":"V Nesin","year":"2012","unstructured":"Nesin, V., Bowman, A.M., Xiao, S., Pakhomov, A.G.: Cell permeabilization and inhibition of voltage-gated Ca(2+) and Na(+) channel currents by nanosecond pulsed electric field. Bioelectromagnetics 33(5), 394\u2013404 (2012)","journal-title":"Bioelectromagnetics"},{"key":"29_CR32","unstructured":"Pakhomov, A.G., Kolb, J., White, J., Shevin, R., Pakhomova, O.N., Schoenbach, K.S.: Membrane effects of ultrashort (nanosecond) electric stimuli. Society for Neuroscience 37th Annual Meeting; Nov. 2\u20137, 2007; San Diego, CA. 2007a. 2007 Neuroscience Meeting Planner CD-ROM, Presentation Number: 317.14"}],"container-title":["Lecture Notes in Computer Science","Intelligent Information and Database Systems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-3-319-15705-4_29","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:16:12Z","timestamp":1747786572000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-3-319-15705-4_29"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"ISBN":["9783319157047","9783319157054"],"references-count":32,"URL":"https:\/\/doi.org\/10.1007\/978-3-319-15705-4_29","relation":{},"ISSN":["0302-9743","1611-3349"],"issn-type":[{"type":"print","value":"0302-9743"},{"type":"electronic","value":"1611-3349"}],"subject":[],"published":{"date-parts":[[2015]]},"assertion":[{"value":"17 March 2015","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}