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Inftech."],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this paper, we present microfluidic chip platforms to enable electrophysiological measurements of nanometer-sized extracellular vesicles. The basis of the chip platform is the realization of a\u00a0synthetic free-standing lipid bilayer spanned within a\u00a0microfabricated aperture. To allow ion channel current measurements, the background current noise level should be reduced to a\u00a0minimum. This can be realized by coating microfabricated apertures from silicon, silicon oxide, or silicon nitride with PTFE or Parylene. Three promising chip platform designs are presented. Electrophysiological measurements conducted with these microfluidic systems show gating events of membrane proteins fused into synthetic lipid bilayers.<\/jats:p>","DOI":"10.1007\/s00502-022-01045-w","type":"journal-article","created":{"date-parts":[[2022,8,19]],"date-time":"2022-08-19T17:08:51Z","timestamp":1660928931000},"page":"471-476","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chip platforms with synthetic lipid bilayers for electrophysiological analyses of pore proteins and extracellular vesicles","Chip-Plattformen mit synthetischen Lipid-Bilayern zur elektrophysiologischen Analyse von Membran-Proteinen und extrazellul\u00e4ren Vesikeln"],"prefix":"10.1007","volume":"139","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1879-2777","authenticated-orcid":false,"given":"Sander","family":"van den Driesche","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Vellekoop","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,8,19]]},"reference":[{"key":"1045_CR1","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.1007\/s00018-018-2992-8","volume":"76","author":"C Brito","year":"2019","unstructured":"Brito\u00a0C, Cabanes\u00a0D, Sarmento Mesquita\u00a0F, Sousa\u00a0S (2019) Mechanisms protecting host cells against bacterial pore-forming toxins. 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