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Computer modeling-guided point mutagenesis of\n                    <jats:italic>C. glutamicum<\/jats:italic>\n                    \u03c3\n                    <jats:sup>H<\/jats:sup>\n                    subunit led to the creation of a panel of \u03c3\n                    <jats:sup>H<\/jats:sup>\n                    variants. Their ability to initiate transcription from naturally occurring hybrid \u03c3\n                    <jats:sup>D<\/jats:sup>\n                    \/\u03c3\n                    <jats:sup>H<\/jats:sup>\n                    -dependent promoter P\n                    <jats:italic>cg0441<\/jats:italic>\n                    and two control canonical promoters (\u03c3\n                    <jats:sup>D<\/jats:sup>\n                    -dependent P\n                    <jats:italic>rsdA<\/jats:italic>\n                    and \u03c3\n                    <jats:sup>H<\/jats:sup>\n                    -dependent P\n                    <jats:italic>uvrD3<\/jats:italic>\n                    ) was measured and interpreted using molecular dynamics simulations of homology models of all complexes. The results led us to design the artificial hybrid promoter P\n                    <jats:italic>D<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>35<\/jats:italic>\n                    <\/jats:sub>\n                    <jats:italic>H<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>10<\/jats:italic>\n                    <\/jats:sub>\n                    combining the\u2009\u221210 element of the P\n                    <jats:italic>uvrD3<\/jats:italic>\n                    promoter and the\u2009\u221235 element of the P\n                    <jats:italic>rsdA<\/jats:italic>\n                    promoter. This artificial hybrid promoter P\n                    <jats:italic>D<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>35-rsdA<\/jats:italic>\n                    <\/jats:sub>\n                    <jats:italic>H<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>10-uvrD3<\/jats:italic>\n                    <\/jats:sub>\n                    showed almost optimal properties needed for the bio-orthogonal transcription (not interfering with the native biological processes).\n                  <\/jats:p>","DOI":"10.1007\/s10822-024-00577-x","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T03:33:29Z","timestamp":1732505609000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Promoter recognition specificity of Corynebacterium glutamicum stress response sigma factors \u03c3D and \u03c3H deciphered using computer modeling and point mutagenesis"],"prefix":"10.1007","volume":"39","author":[{"given":"J.","family":"Blumenstein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Dost\u00e1lov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Ruck\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"\u0160t\u011bp\u00e1nek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Busche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Kalinowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"P\u00e1tek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0802-1346","authenticated-orcid":false,"given":"I.","family":"Barv\u00edk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,11,25]]},"reference":[{"key":"577_CR1","first-page":"331","volume":"50","author":"S Kinoshita","year":"2004","unstructured":"Kinoshita S, Udaka S, Shimono M (2004) Studies on the amino acid fermentation. 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