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The machine vision technology is used to solve the problems of low positioning accuracy and long positioning time in the traditional motion three-dimensional teaching method. The work of this method is as follows: (a) using machine vision method to determine the world coordinate system of the image; (b) using MRF algorithm to extract the features of 3D teaching action image; (c) determining the spatial correlation of 3D teaching action data. In the three-dimensional teaching action image, the smooth filtering technology is used to suppress and eliminate the noise. Then the convolution neural network (CNN) is used to reconstruct the three-dimensional teaching action image. The entropy of three-dimensional teaching behavior of physical education is determined by CNN. Through a large number of computer simulations, the effectiveness of the proposed system is confirmed. 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