{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T12:23:28Z","timestamp":1740140608628,"version":"3.37.3"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T00:00:00Z","timestamp":1661558400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T00:00:00Z","timestamp":1661558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100012957","name":"Friedrich-Schiller-Universit\u00e4t Jena","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100012957","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Theory Biosci."],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fifty years ago, the enigmatic Brazilian myxomycete-species <jats:italic>Didymium aquatile<\/jats:italic> was described and analyzed with respect to the structure of the plasmodium and its spores. In this study, we compare this rare plasmodial slime mold with another, temporarily aquatic taxon from Europe, <jats:italic>Didymium nigripes<\/jats:italic>. Phenotypic plasticity of <jats:italic>D. nigripes<\/jats:italic> was investigated under various environmental conditions. Large changes in the morphology of the plasmodia were observed. For species identification, characteristics of the fruiting bodies are key features. However, <jats:italic>Didymium aquatile<\/jats:italic> was only characterized by its \u201cabnormal\u201d plasmodia, but no molecular data were available. Here, we analyzed DNA-sequences of 22 species of the genera <jats:italic>Didymium<\/jats:italic> and <jats:italic>Diderma<\/jats:italic> with a focus on this South American taxon via molecular genetics. A comparison of 18S-rDNA-sequences from <jats:italic>D.<\/jats:italic>\u00a0<jats:italic>aquatile<\/jats:italic> and 21 other <jats:italic>Didymium<\/jats:italic> (and <jats:italic>Diderma<\/jats:italic>)-species indicates that <jats:italic>D.<\/jats:italic>\u00a0<jats:italic>aquatile<\/jats:italic> is a reproductively isolated morpho-species. Phenotypic plasticity of <jats:italic>D. nigripes<\/jats:italic> is documented with respect to plasmodium morphology and the formation of fruiting bodies, as an example of an adaptation of a terrestrial species to aquatic environments.<\/jats:p>","DOI":"10.1007\/s12064-022-00375-9","type":"journal-article","created":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T08:02:35Z","timestamp":1661587355000},"page":"313-319","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phenotypic plasticity in plasmodial slime molds and molecular phylogeny of terrestrial vs. aquatic species"],"prefix":"10.1007","volume":"141","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5764-6986","authenticated-orcid":false,"given":"T.","family":"Hoppe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"Kutschera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,8,27]]},"reference":[{"key":"375_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/00275514.1960.12024875","volume":"52","author":"A Alexopoulos","year":"1960","unstructured":"Alexopoulos A (1960) Gross morphology of the plasmodium and its possible significance in the relationships among the myxomycetes. Mycologia 52:1\u201320","journal-title":"Mycologia"},{"key":"375_CR2","first-page":"165","volume":"6","author":"H Baba","year":"2018","unstructured":"Baba H, Sevindik M (2018) The roles of myxomycetes in ecosystems. J Bacteriol Mycol 6:165\u2013166","journal-title":"J Bacteriol Mycol"},{"key":"375_CR3","first-page":"17","volume":"41","author":"F Bellido","year":"2017","unstructured":"Bellido F, Moreno G, Meyer M, Castillo A (2017) A new species of Didymium from Spain. Bol Soc Micol Madrid 41:17\u201322","journal-title":"Bol Soc Micol Madrid"},{"key":"375_CR4","first-page":"209","volume":"74","author":"J Clark","year":"2004","unstructured":"Clark J (2004) Reproductive system and taxonomy in the myxomycetes. Syst Geogr Pl 74:209\u2013216","journal-title":"Syst Geogr Pl"},{"key":"375_CR5","doi-asserted-by":"publisher","first-page":"233","DOI":"10.5943\/mycosphere\/4\/2\/6","volume":"4","author":"J Clark","year":"2013","unstructured":"Clark J, Haskins EF (2013) The nuclear reproductive circle in the myxomycetes: a review. Mycosphere 4:233\u2013248","journal-title":"Mycosphere"},{"key":"375_CR6","doi-asserted-by":"publisher","first-page":"153","DOI":"10.5943\/mycosphere\/5\/1\/7","volume":"5","author":"J Clark","year":"2014","unstructured":"Clark J, Haskins EF (2014) Sporophore morphology and development in the myxomycetes: a review. Mycosphere 5:153\u2013170","journal-title":"Mycosphere"},{"key":"375_CR7","first-page":"1","volume":"10","author":"MB Dahl","year":"2017","unstructured":"Dahl MB, Brejnrod AK, Unterseher M, Hoppe T, Feng Y, Novozhilov Y, S\u00f8rensen SJ, Schnittler M (2017) Genetic barcoding of dark-spored myxomycetes (Amoebozoa)\u2013identification, evaluation and application of a sequence similarity threshold for species differentiation in NGS studies. Mol Ecol Resour 10:1\u201313","journal-title":"Mol Ecol Resour"},{"key":"375_CR8","first-page":"331","volume":"2","author":"A Feest","year":"1987","unstructured":"Feest A (1987) The quantitative ecology of soil Mycetozoa. Prog Protistol 2:331\u2013361","journal-title":"Prog Protistol"},{"key":"375_CR9","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/j.protis.2016.03.003","volume":"167","author":"Y Feng","year":"2016","unstructured":"Feng Y, Klahr A, Janik P, Ronikier A, Hoppe T, Novozhilov YK, Schnittler M (2016) What an intron may tell: several sexual biospecies coexist in Meriderma spp. (Myxomycetes). Protist 167:234\u2013253","journal-title":"Protist"},{"key":"375_CR10","volume-title":"The genetical theory of natural selection","author":"RA Fisher","year":"1958","unstructured":"Fisher RA (1958) The genetical theory of natural selection. Dover Publications, New York (second edition)"},{"key":"375_CR11","unstructured":"Fiore-Donno AM, Pawlosky J, Baldauf S, Cavalier-Smith J (2007) Evolutionary pathways in fruiting amoebae (Mycetozoa). In: Abstracts of V European Congress of Protistology and XI European Conference on Ciliate Biology. Protistology 5:29"},{"key":"375_CR12","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1016\/S0007-1536(78)80046-1","volume":"70","author":"TRG Gray","year":"1978","unstructured":"Gray TRG, Lanning S (1978) Culture of an aquatic form of Didymium difforme. Trans Br Mycol Soc 70:289\u2013291","journal-title":"Trans Br Mycol Soc"},{"key":"375_CR13","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1111\/j.1365-2435.2007.01283.x","volume":"21","author":"CK Ghalambor","year":"2007","unstructured":"Ghalambor CK, McKay JK, Carroll SP, Reznick DN (2007) Adaptive versus non-adaptive phenotypic plasticity and the potential for contemporary adaptation in new environments. Funct Ecol 21:394\u2013407","journal-title":"Funct Ecol"},{"key":"375_CR15","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/j.myc.2012.11.001","volume":"54","author":"T Hoppe","year":"2013","unstructured":"Hoppe T (2013) Molecular diversity of myxomycetes near Siegen (Germany). Mycoscience 54:309\u2013313","journal-title":"Mycoscience"},{"key":"375_CR16","doi-asserted-by":"publisher","first-page":"1975","DOI":"10.5943\/mycosphere\/8\/10\/17","volume":"8","author":"T Hoppe","year":"2017","unstructured":"Hoppe T (2017) What is the best? A four marker phylogenetic study of the dark-spored myxomycete Fuligo septica. Mycosphere 8:1975\u20131983","journal-title":"Mycosphere"},{"key":"375_CR14","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1007\/s12064-009-0079-7","volume":"129","author":"T Hoppe","year":"2010","unstructured":"Hoppe T, Kutschera U (2010) In the shadow of Darwin: Anton de Bary\u2019s origin of myxomycetology and a molecular phylogeny of the plasmodial slime molds. Theory Biosci 129:15\u201323","journal-title":"Theory Biosci"},{"key":"375_CR17","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2015.1074368","volume":"10","author":"T Hoppe","year":"2015","unstructured":"Hoppe T, Kutschera U (2015) Species-specific cell mobility of bacteria-feeding myxamoebae in plasmodial slime molds. Plant Signal & Behav 10:e1074268","journal-title":"Plant Signal & Behav"},{"key":"375_CR20","doi-asserted-by":"publisher","first-page":"216","DOI":"10.5943\/mycosphere\/6\/2\/11","volume":"6","author":"T Hoppe","year":"2015","unstructured":"Hoppe T, Schnittler M (2015) Characterization of myxomycetes in two different soils by TRFLP-analysis of 18S rRNA. Mycosphere 6:216\u2013227","journal-title":"Mycosphere"},{"key":"375_CR19","first-page":"131","volume":"23","author":"T Hoppe","year":"2014","unstructured":"Hoppe T, Ammon L, Moll JK (2014) Diversity of lignicolous myxomycetes in young timber forests of Western Germany. Austrian Journal of Mycology 23:131\u2013141","journal-title":"Austrian Journal of Mycology"},{"key":"375_CR21","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1111\/1574-6941.12042","volume":"84","author":"A Kamono","year":"2013","unstructured":"Kamono A, Meyer M, Cavalier-Smith T, Fukui M, Fiore-Donno AM (2013) Exploring slime mould diversity in high-altitude forests and grasslands by environmental RNA analysis. FEMS Microbiol Ecol 84:98\u2013109","journal-title":"FEMS Microbiol Ecol"},{"key":"375_CR22","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/S0269-915X(09)80586-7","volume":"6","author":"T Kappel","year":"1992","unstructured":"Kappel T, Anken RH (1992) An Aquarium myxomycete: Didymium nigripes. The Mycologist 6:106\u2013107","journal-title":"The Mycologist"},{"issue":"1","key":"375_CR18","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1007\/s12064-019-00285-3","volume":"138","author":"U Kutschera","year":"2019","unstructured":"Kutschera U, Hoppe T (2019) Plasmodial slime molds and the evolution of microbial husbandry. Theory Biosci 138(1):127\u2013132. https:\/\/doi.org\/10.1007\/s12064-019-00285-3","journal-title":"Theory Biosci"},{"key":"375_CR23","first-page":"1","volume":"16","author":"C Lado","year":"2001","unstructured":"Lado C (2001) Nomenmyx. A nomenclatural taxabase of myxomycetes. Cuaderos De Trabajo De Flora Micol\u00f3gica Ib\u00e9rica 16:1\u2013221","journal-title":"Cuaderos De Trabajo De Flora Micol\u00f3gica Ib\u00e9rica"},{"key":"375_CR25","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1080\/15572536.2007.11832544","volume":"99","author":"LA Lindley","year":"2007","unstructured":"Lindley LA, Stephenson SL, Spiegel FW (2007) Protostelids and myxomycetes isolated from aquatic habitats. Mycologia 99:504\u2013509","journal-title":"Mycologia"},{"key":"375_CR26","volume-title":"The Myxomycetes","author":"GW Martin","year":"1969","unstructured":"Martin GW, Alexopoulos CJ (1969) The Myxomycetes. Univ Iowa Press, Chicago"},{"key":"375_CR27","first-page":"80","volume":"6","author":"H M\u00fcller","year":"2008","unstructured":"M\u00fcller H, Hoppe T, Ferchen T (2008) Didymium nigripes - Notiz \u00fcber einen aquatischen Schleimpilz. Deutsche Aquaristische Zeitschrift 6:80\u201381","journal-title":"Deutsche Aquaristische Zeitschrift"},{"key":"375_CR28","first-page":"13","volume":"74","author":"NE Nannenga-Bremekamp","year":"1971","unstructured":"Nannenga-Bremekamp NE, Gottsberger G (1971) A new species of Didymium from brazil. KNA Wet Amst 74:13\u201317","journal-title":"KNA Wet Amst"},{"key":"375_CR29","doi-asserted-by":"publisher","first-page":"2362","DOI":"10.1242\/jeb.02070","volume":"209","author":"M Pigliucci","year":"2006","unstructured":"Pigliucci M, Murren CJ, Schlichting CD (2006) Phenotypic plasticity and evolution by genetic assimilation. J Exp Biol 209:2362\u20132367","journal-title":"J Exp Biol"},{"key":"375_CR30","first-page":"55","volume":"73","author":"M Schnittler","year":"2000","unstructured":"Schnittler M, Mitchell DW (2000) Species diversity in myxomycetes based on the morphological species concept \u2013 a critical comparison. Stapfia 73:55\u201362","journal-title":"Stapfia"},{"key":"375_CR31","volume-title":"Myxomycetes \u2013 biology, systematics, biogeography, and ecology","author":"SL Stephenson","year":"2017","unstructured":"Stephenson SL, Rojas C (2017) Myxomycetes \u2013 biology, systematics, biogeography, and ecology. Elsevier, London, San Diego, Cambridge Oxford"},{"key":"375_CR32","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1007\/s10531-007-9252-9","volume":"17","author":"SL Stephenson","year":"2008","unstructured":"Stephenson SL, Schnittler M, Novozhilov YK (2008) Myxomycete diversity and distribution from the fossil record to the present. Biodiv Conserv 17:285\u2013301","journal-title":"Biodiv Conserv"},{"key":"375_CR33","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1002\/j.1537-2197.1977.tb15729.x","volume":"64","author":"CD Therrien","year":"1977","unstructured":"Therrien CD, Bell WR, Collins O\u2019Neil R (1977) Nuclear DNA content of myxamoebae and plasmodia in six non-heterothallic isolates of a myxomycete, Didymium iridis. Amer J Bot 64:286\u2013291","journal-title":"Amer J Bot"},{"key":"375_CR34","first-page":"64","volume":"24","author":"MH Ward","year":"1886","unstructured":"Ward MH (1886) The morphology and physiology of an aquatic myxomycete. Quarter J Microscop Sci 24:64\u201386","journal-title":"Quarter J Microscop Sci"},{"key":"375_CR35","first-page":"287","volume":"6","author":"KE Winsett","year":"2013","unstructured":"Winsett KE, Stephenson SL (2013) Myxomycetes isolated from submerged plant material collected in the Big Thicket National Preserve, Texas. Mycosphere 6:287\u2013298","journal-title":"Mycosphere"}],"container-title":["Theory in Biosciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12064-022-00375-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12064-022-00375-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12064-022-00375-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T17:51:52Z","timestamp":1663177912000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12064-022-00375-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,27]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["375"],"URL":"https:\/\/doi.org\/10.1007\/s12064-022-00375-9","relation":{},"ISSN":["1431-7613","1611-7530"],"issn-type":[{"type":"print","value":"1431-7613"},{"type":"electronic","value":"1611-7530"}],"subject":[],"published":{"date-parts":[[2022,8,27]]},"assertion":[{"value":"1 February 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 August 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}