{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:36:16Z","timestamp":1772120176425,"version":"3.50.1"},"reference-count":10,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000888","name":"W. M. Keck Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000888","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Quant. Biol."],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Multiplexed milliliter\u2010scale chemostats are useful for measuring cell physiology under various degrees of nutrient limitation and for carrying out evolution experiments. In each chemostat, fresh medium containing a growth rate\u2010limiting metabolite is pumped into the culturing chamber at a constant rate, while culture effluent exits at an equal rate. Although such devices have been developed by various labs, key parameters \u2014 the accuracy, precision, and operational range of flow rate \u2014 are not explicitly characterized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Here we re\u2010purpose a published multiplexed culturing device to develop a multiplexed milliliter\u2010scale chemostat. Flow rates for eight chambers can be independently controlled to a wide range, corresponding to population doubling times of 3~13 h, without the use of expensive feedback systems.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Flow rates are precise, with the maximal coefficient of variation among eight chambers being less than 3%. Flow rates are accurate, with average flow rates being only slightly below targets,\n                      <jats:italic>i.e.,<\/jats:italic>\n                      3%\u20136% for 13\u2010h and 0.6%\u20131.0% for 3\n\u2010h doubling times. This deficit is largely due to evaporation and should be correctable. We experimentally demonstrate that our device allows accurate and precise quantification of population phenotypes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We achieve precise control of cellular growth in a low\u2010cost milliliter\u2010scale chemostat array, and show that the achieved precision reduces the error when measuring biological processes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s40484-018-0143-8","type":"journal-article","created":{"date-parts":[[2018,7,26]],"date-time":"2018-07-26T05:19:03Z","timestamp":1532582343000},"page":"129-141","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Developing a low\u2010cost milliliter\u2010scale chemostat array for precise control of cellular growth"],"prefix":"10.1002","volume":"6","author":[{"given":"David","family":"Skelding","sequence":"first","affiliation":[{"name":"<!--1--> Division of Basic Sciences Fred Hutchinson Cancer Research Center Seattle WA 98109 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel F M","family":"Hart","sequence":"additional","affiliation":[{"name":"<!--1--> Division of Basic Sciences Fred Hutchinson Cancer Research Center Seattle WA 98109 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thejas","family":"Vidyasagar","sequence":"additional","affiliation":[{"name":"<!--2--> University of Washington Seattle WA 98195\u20103770 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander E","family":"Pozhitkov","sequence":"additional","affiliation":[{"name":"<!--1--> Division of Basic Sciences Fred Hutchinson Cancer Research Center Seattle WA 98109 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenying","family":"Shou","sequence":"additional","affiliation":[{"name":"<!--1--> Division of Basic Sciences Fred Hutchinson Cancer Research Center Seattle WA 98109 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,6]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.5020220206"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/sb500165g"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.36.12.708"},{"key":"e_1_2_8_5_2","article-title":"Design and use of multiplexed chemostat arrays","volume":"72","author":"Miller A.W.","year":"2013","journal-title":"JoVEJ. 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