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Biol."],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Traditionally, scientists studied microbiology through the manner of batch cultures, to conclude the dynamics or outputs by averaging all individuals. However, as the researches go further, the heterogeneities among the individuals have been proven to be crucial for the population dynamics and fates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Due to the limit of technology, single\u2010cell analysis methods were not widely used to decipher the inherent connections between individual cells and populations. Since the early decades of this century, the rapid development of microfluidics, fluorescent labelling, next\u2010generation sequencing, and high\u2010resolution microscopy have speeded up the development of single\u2010cell technologies and further facilitated the applications of these technologies on bacterial analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In this review, we summarized the recent processes of single\u2010cell technologies applied in bacterial analysis in terms of intracellular characteristics, cell physiology dynamics, and group behaviors, and discussed how single\u2010cell technologies could be more applicable for future bacterial 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University of Chinese Academy of Sciences Beijing 100049 China"}]},{"given":"Yingtong","family":"Su","sequence":"additional","affiliation":[{"name":"<!--1--> Shenzhen Institute of Synthetic Biology Shenzhen Institutes of Advance Technology Chinese Academy of Sciences Shenzhen 518055 China"},{"name":"<!--2--> University of Chinese Academy of Sciences Beijing 100049 China"}]},{"given":"Yue","family":"Yu","sequence":"additional","affiliation":[{"name":"<!--1--> Shenzhen Institute of Synthetic Biology Shenzhen Institutes of Advance Technology Chinese Academy of Sciences Shenzhen 518055 China"}]},{"given":"Hui","family":"Wen","sequence":"additional","affiliation":[{"name":"<!--1--> Shenzhen Institute of Synthetic Biology Shenzhen Institutes of Advance Technology Chinese Academy of Sciences Shenzhen 518055 China"}]},{"given":"Xiongfei","family":"Fu","sequence":"additional","affiliation":[{"name":"<!--1--> Shenzhen Institute of Synthetic Biology Shenzhen Institutes of Advance Technology 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Chu, Yingtong Su, Yue Yu, Hui Wen, Xiongfei Fu and Shuqiang Huang declare that they have no conflict of interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethics Guidelines"}},{"value":"This article is a review article and does not contain any studies with human or animal subjects performed by any of the authors.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethics Guidelines"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}