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Biol."],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Time\u2010lapse live cell imaging of a growing cell population is routine in many biological investigations. A major challenge in imaging analysis is accurate segmentation, a process to define the boundaries of cells based on raw image data. Current segmentation methods relying on single boundary features have problems in robustness when dealing with inhomogeneous foci which invariably happens in cell population imaging.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Combined with a multi\u2010layer training set strategy, we developed a neural\u2010network\u2010based algorithm \u2014 Cellbow.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Cellbow can achieve accurate and robust segmentation of cells in broad and general settings. It can also facilitate long\u2010term tracking of cell growth and division. To facilitate the application of Cellbow, we provide a website on which one can online test the software, as well as an ImageJ plugin for the user to visualize the performance before software installation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Cellbow is customizable and generalizable. It is broadly applicable to segmenting fluorescent images of diverse cell types with no further training needed. For bright\u2010field images, only a small set of sample images of the specific cell type from the user may be needed for training.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s40484-020-0213-6","type":"journal-article","created":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T10:03:58Z","timestamp":1599127438000},"page":"245-255","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cellbow: a robust customizable cell segmentation program"],"prefix":"10.1002","volume":"8","author":[{"given":"Huixia","family":"Ren","sequence":"first","affiliation":[{"name":"Center for Quantitative Biology and Peking\u2010Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China"}]},{"given":"Mengdi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center for Quantitative Biology and Peking\u2010Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China"}]},{"given":"Bo","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Quantitative Biology and Peking\u2010Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China"}]},{"given":"Ruixiao","family":"Yao","sequence":"additional","affiliation":[{"name":"Center for Quantitative Biology and Peking\u2010Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China"}]},{"given":"Qi","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Quantitative Biology and Peking\u2010Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China"}]},{"given":"Zhipeng","family":"Ren","sequence":"additional","affiliation":[{"name":"Center for Quantitative Biology and Peking\u2010Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China"}]},{"given":"Zirui","family":"Wu","sequence":"additional","affiliation":[{"name":"Center for Quantitative Biology and Peking\u2010Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China"}]},{"given":"Zongmao","family":"Gao","sequence":"additional","affiliation":[{"name":"Center for Quantitative Biology and Peking\u2010Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China"}]},{"given":"Xiaojing","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Quantitative Biology and Peking\u2010Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China"}]},{"given":"Chao","family":"Tang","sequence":"additional","affiliation":[{"name":"Center for Quantitative Biology and Peking\u2010Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China"}]}],"member":"311","published-online":{"date-parts":[[2020,9]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3550"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003043"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu302"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005177"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb\u20102006\u20107\u201010\u2010r100"},{"key":"e_1_2_7_7_2","doi-asserted-by":"crossref","unstructured":"O\u2019Brien J. Hoque S. Mulvihill D.andSirlantzis K.(2017)Automated cell segmentation of fission yeast phase images\u2014segmenting cells from light microscopy images.In:Proc.10th Inter. Joint Conf. Biomed. Eng. Syst. Technol. pp.92\u201399","DOI":"10.5220\/0006149100920099"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2016.0705"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1109\/MSP.2012.2204190"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1751\u20100473\u20108\u201016"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0206395"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx550"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081434"},{"key":"e_1_2_7_14_2","doi-asserted-by":"crossref","unstructured":"Peng J. Y. Chen Y. J. Green M. D. Forsburg S. L.andHsu C. N.(2013)Robust cell segmentation for schizosaccharomyces pombe images with focus gradient.In:Proc. Inter. Sympo. on Biomed. Imag.doi:10.1109\/ISBI.2013.6556500.","DOI":"10.1109\/ISBI.2013.6556500"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-84882-087-6_9"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1109\/CVPR.2018.00712"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1109\/TPAMI.2016.2572683"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12859\u2010015\u20100762\u20102"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2083"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1109\/TII.2016.2542043"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.patcog.2015.12.009"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165\u20101684(94)90060\u20104"},{"key":"e_1_2_7_23_2","unstructured":"Abadi M. Agarwal A. Barham P. 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