{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:17:29Z","timestamp":1760782649855},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"1-2","license":[{"start":{"date-parts":[[1995,12,1]],"date-time":"1995-12-01T00:00:00Z","timestamp":817776000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[1995,12]]},"DOI":"10.1007\/bf00164481","type":"journal-article","created":{"date-parts":[[2004,11,8]],"date-time":"2004-11-08T11:43:32Z","timestamp":1099914212000},"page":"59-67","source":"Crossref","is-referenced-by-count":25,"title":["CMP-N-acetyl neuraminic-acid synthetase from Escherichia coli: fermentative production and application for the preparative synthesis of CMP-neuraminic acid"],"prefix":"10.1007","volume":"44","author":[{"given":"M.","family":"Kittelmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"Kragl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Wandrey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Ghisalba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"key":"CR1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1002\/9780470122860.ch3","volume":"41","author":"G Ashwell","year":"1974","unstructured":"Ashwell G, Morell AG (1974) The role of surface carbohydrate in the hepatic recognition and transport of circulating glycoproteins. Adv Enzymol Related Areas Mol Biol 41:99?128","journal-title":"Adv Enzymol Related Areas Mol Biol"},{"key":"CR2","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/S0040-4039(00)80210-5","volume":"29","author":"C Aug\ufffd","year":"1988","unstructured":"Aug\ufffd C, Gautheron C (1988) An efficient synthesis of cytidine monophospho-sialic acids with four immobilized enzymes. Tetrahedron Lett 29:789?790","journal-title":"Tetrahedron Lett"},{"key":"CR3","unstructured":"Decorte E, Miccoli P (1984) Pharmaceutical composition containing the cytidine monophosphate of 5-acetamido-3,5-dideoxy-d-glycerol-d-galactononulosaminic acid. European patent specification 0 120 328 B1"},{"key":"CR4","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1111\/j.1432-1033.1987.tb13458.x","volume":"168","author":"HJ Gross","year":"1987","unstructured":"Gross HJ, B\ufffdnsch A, Paulson CP, Brossmer R (1987) Activation and transfer of novel synthetic 9-substituted sialic acids. Eur J Biochem 168:595?602","journal-title":"Eur J Biochem"},{"key":"CR5","doi-asserted-by":"crossref","first-page":"8838","DOI":"10.1016\/S0021-9258(17)39428-0","volume":"260","author":"HH Higa","year":"1985","unstructured":"Higa HH, Paulson JC (1985) Sialylation of glycoprotein oligosacharides with N-acetyl-, N-glycolyl-, and N-0-diacetyl-neuraminic acids. J Biol Chem 260:8838?8849","journal-title":"J Biol Chem"},{"key":"CR6","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1007\/BF00731185","volume":"10","author":"CT Holzer","year":"1993","unstructured":"Holzer CT, Itzstein M von, Jin B, Pegg MS, Stewart WP, Wu W-Y (1993) Inhibition of sialidases from viral, bacterial, and mammalian source by analogues of 2-deoxy-2,3-didehydro-N-acetyl-neuraminic acid modified at the C-4 position. Glycoconjugate J 10:40?44","journal-title":"Glycoconjugate J"},{"key":"CR7","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/0003-2697(76)90560-1","volume":"72","author":"W H\ufffdnig","year":"1976","unstructured":"H\ufffdnig W, Kula M-R (1976) Selectivity of protein precipication with polyethylene glycol fractions of various molecular weights. Anal Biochem 72:502?512","journal-title":"Anal Biochem"},{"key":"CR8","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1128\/JB.105.1.160-164.1971","volume":"105","author":"NE Kelker","year":"1971","unstructured":"Kelker NE, Anderson L (1971) Sorbitol metabolism in Aerobacter aerogenes. J Bacteriol 105:160?164","journal-title":"J Bacteriol"},{"key":"CR9","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1111\/j.1749-6632.1992.tb32711.x","volume":"672","author":"M Kittelmann","year":"1992","unstructured":"Kittelmann M, Klein T, Kragl U, Wandrey C, Ghisalba O (1992) Preparative enzymatic synthesis of activated neuraminic acid using a microbial enzyme. Ann NY Acad Sci 672:444?450","journal-title":"Ann NY Acad Sci"},{"key":"CR10","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1002\/anie.199108271","volume":"30","author":"U Kragl","year":"1991","unstructured":"Kragl U, Gygax D, Ghisalba O, Wandrey C (1991) Enzymatic two step synthesis of N-acetyl-neuraminic acid in the enzyme membrane reactor. Angew Chem Int Ed Engl 30:827?828","journal-title":"Angew Chem Int Ed Engl"},{"key":"CR11","doi-asserted-by":"crossref","unstructured":"Kragl U, Kittelmann M, Ghisalba O, Wandrey C (1995) N-Acetyl-neuraminic acid: from a rare chemical to a multi kilogram enzymatic synthesis for industrial application (Proceedings of the Enzyme Engineering Conference XII, Deauville, France). Ann NY Acad Sci (in press)","DOI":"10.1111\/j.1749-6632.1995.tb19969.x"},{"key":"CR12","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1351\/pac198456070797","volume":"56","author":"JC Paulson","year":"1984","unstructured":"Paulson JC, Rogers GN, Carroll SM, Higa HH, Pritchett T, Milks G, Sabesan S (1984) Selection of influenza virus variant based on sialyloligosaccharide receptor specifity. Pure Appl Chem 56:797?805","journal-title":"Pure Appl Chem"},{"key":"CR13","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1515\/bchm2.1972.353.1.883","volume":"353","author":"R Schauer","year":"1972","unstructured":"Schauer R, Wember M, Do Amaral CF (1972) Synthesis of CMP-glycosides of radioactive of N-acetyl, N-glycolyl-, N-acetyl-7-0-acety-and N-acetyl-8-O-acetylneuraminic acids by CMP-sialate synthase from bovine submaxillary glands. Hoppe Seyler's Z Physiol Chem 353:883?886","journal-title":"Hoppe Seyler's Z Physiol Chem"},{"key":"CR14","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1093\/glycob\/1.2.187","volume":"1","author":"SL Shames","year":"1991","unstructured":"Shames SL, Simon ES, Christopher CW, Schmid W, Whitesides GM, Yang LL (1991) CMP-N-acetylneuraminic acid synthetase of Escherichia coli: high level expression, purification, and use in the enzymatic synthesis of CMP-N-acetylneuraminic acid and CMP-neuraminic acid derivatives. Glycobiology 1: 187?191","journal-title":"Glycobiology"},{"key":"CR15","doi-asserted-by":"crossref","first-page":"31","DOI":"10.3109\/10242429209014880","volume":"6","author":"G-J Shen","year":"1992","unstructured":"Shen G-J, Liu JL-C, Wong C-H (1992) Cloning and overexpression of a tagged CMP-N-acetylneuraminic acid synthetase from E. coli using a lambda phage system and application of the enzyme to the synthesis of CMP-N-acetylneuraminic acid. Biocatalysis 6:31?42","journal-title":"Biocatalysis"},{"key":"CR16","doi-asserted-by":"crossref","first-page":"7159","DOI":"10.1021\/ja00229a033","volume":"110","author":"ES Simon","year":"1988","unstructured":"Simon ES, Bednarski MD, Whitesides GM (1988) Synthesis of CMP-NeuAc from N-acetylglucosamine: generation of CTP from CMP using adenylate kinase. J Am Chem Soc 110:7159?7163","journal-title":"J Am Chem Soc"},{"key":"CR17","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0076-6879(89)79129-1","volume":"179","author":"ES Simon","year":"1989","unstructured":"Simon ES, Toone EJ, Ostroff G, Bednarski MD, Whitesides GM (1989) Preparation of cytidine 5?monophospho-N-acetylneuraminic acid and uridine 5?-diphosphoglucuronic acid; syntheses of alpha-2,6-sialyllactosamine, alpha-2,6-sialyllactose, and hyaluronic acid. Methods Enzymol 179:275?287","journal-title":"Methods Enzymol"},{"key":"CR18","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1002\/jlac.1990199001199","volume":"1990","author":"J Thiem","year":"1990","unstructured":"Thiem J, Stangier P (1990) Preparative enzymatic formation of cytidine 5?-monophosphosialate by integrated cytidine 5?-triphosphate regeneration. Liebigs Ann Chem 1990:1101?1105","journal-title":"Liebigs Ann Chem"},{"key":"CR19","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/0076-6879(82)83050-4","volume":"83","author":"FA Troy","year":"1982","unstructured":"Troy FA, Vijay IK, McCloskey MA, Rohr TE (1982) Synthesis of capsular polymers containing polysialic acidin Escherichia coli O7-K1. Methods Enzymol 83:540?548","journal-title":"Methods Enzymol"},{"key":"CR20","doi-asserted-by":"crossref","first-page":"2105","DOI":"10.1080\/00021369.1973.10860963","volume":"37","author":"Y Uchida","year":"1973","unstructured":"Uchida Y, Tsudaka Y, Sugimori T (1973) Improved microbial production of colominic acid, a homoploymer of N-acetylneuraminic acid. Agric Biol Chem 37:2105?2110","journal-title":"Agric Biol Chem"},{"key":"CR21","doi-asserted-by":"crossref","first-page":"17556","DOI":"10.1016\/S0021-9258(18)45417-8","volume":"262","author":"W Vann","year":"1987","unstructured":"Vann W, Silver RP, Abeijon C, Chang K, Aaronson W, Sutton A, Finn CW, Linder W, Kosatos M (1987) Purification, properties and genetic location of Escherichia coli cytidine 5?-monophosphate N-acetylneuraminic acid synthase. J Biol Chem 262:17556?17562","journal-title":"J Biol Chem"},{"key":"CR22","doi-asserted-by":"crossref","first-page":"3527","DOI":"10.1016\/S0021-9258(19)70851-5","volume":"237","author":"L Warren","year":"1962","unstructured":"Warren L, Blacklow RS (1962) The Biosynthesis of cytidine 5?-monophospho-N-acetylneuraminic acid by an enzyme from Neisseria meningitidis. J Biol Chem 237:3527?3534","journal-title":"J Biol Chem"},{"key":"CR23","first-page":"1969","volume":"128","author":"MJ Woodward","year":"1982","unstructured":"Woodward MJ, Charles HP (1982) Genes for l-sorbose utilization in Escherichia coli. J Gen Microbiol 128:1969?1980","journal-title":"J Gen Microbiol"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/BF00164481.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/BF00164481\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/BF00164481","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T03:04:58Z","timestamp":1705287898000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/BF00164481"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,12]]},"references-count":23,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[1995,12]]}},"alternative-id":["BF00164481"],"URL":"https:\/\/doi.org\/10.1007\/bf00164481","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,12]]}}}