{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T06:50:33Z","timestamp":1780469433538,"version":"3.54.1"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[1990,11,1]],"date-time":"1990-11-01T00:00:00Z","timestamp":657417600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molec. Gen. Genet."],"published-print":{"date-parts":[[1990,11]]},"DOI":"10.1007\/bf00271553","type":"journal-article","created":{"date-parts":[[2004,9,30]],"date-time":"2004-09-30T17:02:05Z","timestamp":1096563725000},"page":"201-208","source":"Crossref","is-referenced-by-count":64,"title":["Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins"],"prefix":"10.1007","volume":"224","author":[{"given":"J.","family":"Hess","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Gentschev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Goebel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Jarchau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","reference":[{"key":"BF00271553_CR1","doi-asserted-by":"crossref","first-page":"2463","DOI":"10.1002\/j.1460-2075.1987.tb02526.x","volume":"6","author":"D Baty","year":"1987","unstructured":"Baty D, Lloubes R, Geli V, Lazdunski C, Howard SP (1987) Extracellular release of colicin A is non-specific. EMBO J 6:2463\u20132468","journal-title":"EMBO J"},{"key":"BF00271553_CR2","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/0022-2836(69)90288-5","volume":"41","author":"HW Boyer","year":"1969","unstructured":"Boyer HW, Roulland-Dussoix D (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459\u2013472","journal-title":"J Mol Biol"},{"key":"BF00271553_CR3","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0735-0651(87)90003-3","volume":"4","author":"P Carter-Muenchau","year":"1987","unstructured":"Carter-Muenchau P, Wolf RE Jr (1987) A method for cloning mixtures of long, synthetic oligonucleotides. Gen Anal Techn 4:105\u2013110","journal-title":"Gen Anal Techn"},{"key":"BF00271553_CR4","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1038\/299371a0","volume":"299","author":"D Eisenberg","year":"1982","unstructured":"Eisenberg D, Weiss RM, Terwilliger TC (1982) The helical hydrophobic moment: a measure of the amphiphilicity of a helix. Nature 299:371\u2013374","journal-title":"Nature"},{"key":"BF00271553_CR5","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1007\/BF00330427","volume":"208","author":"K Erb","year":"1987","unstructured":"Erb K, Vogel M, Wagner W, Goebel W (1987) Alkaline phosphatase which lacks its own signal sequence becomes enzymatically active when fused to N-terminal sequences of Escherichia coli haemolysin (HlyA). Mol Gen Genet 208:88\u201393","journal-title":"Mol Gen Genet"},{"key":"BF00271553_CR6","doi-asserted-by":"crossref","first-page":"5269","DOI":"10.1073\/pnas.85.14.5269","volume":"85","author":"T Felmlee","year":"1988","unstructured":"Felmlee T, Welch RA (1988) Alterations of amino acid repeats in the Escherichia coli hemolysin affect cytolytic activity and secretion. Proc Natl Acad Sci USA 85:5269\u20135273","journal-title":"Proc Natl Acad Sci USA"},{"key":"BF00271553_CR7","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0022-2836(78)90297-8","volume":"120","author":"J Garnier","year":"1978","unstructured":"Garnier J, Osguthorpe DJ, Robson B (1978) Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol 120:97\u2013120","journal-title":"J Mol Biol"},{"key":"BF00271553_CR8","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/BF00633820","volume":"222","author":"I Gentschev","year":"1990","unstructured":"Gentschev I, Hess J, Goebel W (1990) Change in the cellular localization of alkaline phosphatase by alteration of its carboxyterminal sequence. Mol Gen Genet 222:211\u2013216","journal-title":"Mol Gen Genet"},{"key":"BF00271553_CR9","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1128\/JB.151.3.1290-1298.1982","volume":"151","author":"W Goebel","year":"1982","unstructured":"Goebel W, Hedgpeth J (1982) Cloning and functional characterization of the plasmid-encoded hemolysin determinant of Escherichia coli. J Bacteriol 151:1290\u20131298","journal-title":"J Bacteriol"},{"key":"BF00271553_CR10","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1002\/jcb.240220203","volume":"22","author":"M H\u00e4rtlein","year":"1983","unstructured":"H\u00e4rtlein M, Schie\u00dfl S, Wagner W, Rdest U, Kreft J, Goebel W (1983) Transport of hemolysin by Escherichia coli. J Cell Biochem 22:87\u201397","journal-title":"J Cell Biochem"},{"key":"BF00271553_CR11","volume-title":"PhD thesis","author":"J Hess","year":"1990","unstructured":"Hess J (1990) Transport von H\u00e4molysin in Escherichia coli. PhD thesis, University W\u00fcrzburg, FRG"},{"key":"BF00271553_CR12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.1986.tb01339.x","volume":"34","author":"J Hess","year":"1986","unstructured":"Hess J, Wels W, Vogel M, Goebel W (1986) Nucleotide sequence of a plasmid encoded hemolysin determinant and its comparison with a corresponding chromosomal hemolysin sequence. FEMS Microbiol Lett 34:1\u201311","journal-title":"FEMS Microbiol Lett"},{"key":"BF00271553_CR13","first-page":"219","volume-title":"Microbial Products, New Approaches","author":"IB Holland","year":"1989","unstructured":"Holland IB, Wang R, Seror SJ, Blight M (1989) Haemolysin secretion and other protein translocation mechanisms in gram-negative bacteria. In: Baumberg S, Hinster I, Rhodes M (eds) Microbial Products, New Approaches. Cambridge University Press, Cambridge, pp 219\u2013254"},{"key":"BF00271553_CR14","doi-asserted-by":"crossref","first-page":"4789","DOI":"10.1093\/nar\/16.11.4789","volume":"16","author":"V Koronakis","year":"1988","unstructured":"Koronakis V, Cross M, Hughes C (1988) Expression of the E. coli hemolysin secretion gene hlyB involves transcript anti-termination within the hly operon. Nucleic Acids Res 16:4789\u20134800","journal-title":"Nucleic Acids Res"},{"key":"BF00271553_CR15","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1002\/j.1460-2075.1989.tb03414.x","volume":"8","author":"V Koronakis","year":"1989","unstructured":"Koronakis V, Koronakis E, Hughes C (1989) Isolation and analysis of the C-terminal signal directing export of Escherichia coli hemolysin across both bacterial membranes. EMBO J 8:595\u2013605","journal-title":"EMBO J"},{"key":"BF00271553_CR16","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"UK Laemmli","year":"1970","unstructured":"Laemmli UK (1970) Cleavage of structural proteins during the assembly of the heads of bacteriophage T4. Nature 227:680\u2013685","journal-title":"Nature"},{"key":"BF00271553_CR17","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1146\/annurev.cb.02.110186.001531","volume":"2","author":"C Lee","year":"1986","unstructured":"Lee C, Beckwith J (1986) Cotranslational and post-translational protein translocation in procaryotic systems. Annu Rev Cell Biol 2:315\u2013336","journal-title":"Annu Rev Cell Biol"},{"key":"BF00271553_CR18","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1007\/BF00333579","volume":"206","author":"A Ludwig","year":"1987","unstructured":"Ludwig A, Vogel M, Goebel W (1987) Mutations affecting activity and transport of haemolysin in E. coli. Mol Gen Genet 206:238\u2013245","journal-title":"Mol Gen Genet"},{"key":"BF00271553_CR19","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/BF00334104","volume":"196","author":"N Mackman","year":"1984","unstructured":"Mackman N, Nicaud JM, Gray L, Holland IB (1984) Functional characterization of a cloned haemolysin determinant from E. coli of human origin, encoding information for the secretion of a 107 K polypeptide. Mol Gen Genet 196:129\u2013134","journal-title":"Mol Gen Genet"},{"key":"BF00271553_CR20","doi-asserted-by":"crossref","first-page":"2835","DOI":"10.1002\/j.1460-2075.1987.tb02580.x","volume":"6","author":"N Mackman","year":"1987","unstructured":"Mackman N, Baker K, Gray L, Haigh R, Nicaud JM, Holland B (1987) Release of a chimeric protein into the medium from Escherichia coli using the C-terminal secretion signal of haemolysin. EMBO J 6:2835\u20132841","journal-title":"EMBO J"},{"key":"BF00271553_CR21","doi-asserted-by":"crossref","first-page":"8129","DOI":"10.1073\/pnas.82.23.8129","volume":"82","author":"C Manoil","year":"1985","unstructured":"Manoil C, Beckwith J (1985) TnPhoA: A transposon probe for protein export signals. Proc Natl Acad Sci USA 82:8129\u20138133","journal-title":"Proc Natl Acad Sci USA"},{"key":"BF00271553_CR22","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/0014-5793(86)80838-9","volume":"204","author":"JM Nicaud","year":"1986","unstructured":"Nicaud JM, Mackman N, Gray L, Holland IB (1986) The C-terminal 23 kD peptide of Escherichia coli haemolysin 2001 contains all the information necessary for its secretion by the haemolysin (Hly) machinery. FEBS Lett 204:331\u2013335","journal-title":"FEBS Lett"},{"key":"BF00271553_CR23","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/0003-2697(80)90470-4","volume":"105","author":"BR Oakley","year":"1980","unstructured":"Oakley BR, Kirsch DR, Morris NR (1980) A simplified silver stain for detecting proteins in polyacrylamide gels. Anal Biochem 105:361\u2013363","journal-title":"Anal Biochem"},{"key":"BF00271553_CR24","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/0092-8674(81)90184-7","volume":"25","author":"D Oliver","year":"1981","unstructured":"Oliver D, Beckwith J (1981) E. coli mutant pleiotropically defective in the export of secreted proteins. Cell 25:765\u2013772","journal-title":"Cell"},{"key":"BF00271553_CR25","doi-asserted-by":"crossref","first-page":"2783","DOI":"10.1128\/jb.171.5.2783-2788.1989","volume":"171","author":"RL Oropeza-Wekerle","year":"1989","unstructured":"Oropeza-Wekerle RL, M\u00fcller E, Kern P, Meyermann R, Goebel W (1989) Synthesis, inactivation and localization of extracellular and intracellular Escherichia coli hemolysins. J Bacteriol 171:2783\u20132788","journal-title":"J Bacteriol"},{"key":"BF00271553_CR26","doi-asserted-by":"crossref","first-page":"3711","DOI":"10.1128\/jb.172.7.3711-3717.1990","volume":"172","author":"RL Oropeza-Wekerle","year":"1990","unstructured":"Oropeza-Wekerle RL, Speth W, Imhof B, Gentschev I, Goebel W (1990) Translocation and compartmentalization of Escherichia coli hemolysin (HlyA). J Bacteriol 172:3711\u20133717","journal-title":"J Bacteriol"},{"key":"BF00271553_CR27","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1038\/325458a0","volume":"325","author":"J Pohlner","year":"1987","unstructured":"Pohlner J, Halter R, Beyreuther K, Meyer TF (1987) Gene structure and extracellular secretion of Neisseria gonorrhoeae IgA protease. Nature 325:458\u2013462","journal-title":"Nature"},{"key":"BF00271553_CR28","doi-asserted-by":"crossref","unstructured":"Pugsley AP, Reyss I (1990) Five genes at the 3\u2032 end of the Klebsiella pneumoniae pulC operon are required for pullulanase secretion. Mol Microbiol, in press","DOI":"10.1111\/j.1365-2958.1990.tb00604.x"},{"key":"BF00271553_CR29","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1574-6968.1985.tb01180.x","volume":"32","author":"AP Pugsley","year":"1985","unstructured":"Pugsley AP, Schwartz M (1985) Export and secretion of proteins by bacteria. FEMS Microbiol Rev 32:3\u201338","journal-title":"FEMS Microbiol Rev"},{"key":"BF00271553_CR30","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1146\/annurev.mi.41.100187.002451","volume":"41","author":"LL Randall","year":"1987","unstructured":"Randall LL, Hardy SJS, Thom JR (1987) Export of proteins: a biochemical view. Annu Rev Microbiol 41:507\u2013541","journal-title":"Annu Rev Microbiol"},{"key":"BF00271553_CR31","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1128\/MMBR.53.3.333-366.1989","volume":"53","author":"MH Saier","year":"1989","unstructured":"Saier MH, Werner PK, M\u00fcller M (1989) Insertion of proteins into bacterial membranes: mechanism, characteristics and comparisons with the eucaryotic process. Microbiol Rev 53:333\u2013366","journal-title":"Microbiol Rev"},{"key":"BF00271553_CR32","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","volume":"76","author":"H Towbin","year":"1979","unstructured":"Towbin H, Staehlin H, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350\u20134354","journal-title":"Proc Natl Acad Sci USA"},{"key":"BF00271553_CR33","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1007\/BF00322447","volume":"212","author":"M Vogel","year":"1988","unstructured":"Vogel M, Hess J, Then I, Juarez A, Goebel W (1988) Characterization of a sequence (hlyR) which enhances synthesis and secretion of hemolysin in Escherichia coli. Mol Gen Genet 212:76\u201384","journal-title":"Mol Gen Genet"},{"key":"BF00271553_CR34","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1128\/JB.154.1.200-210.1983","volume":"154","author":"W Wagner","year":"1983","unstructured":"Wagner W, Vogel M, Goebel W (1983) Transport of hemolysin across the outer membrane of Escherichia coli requires two functions. J Bacteriol 154:200\u2013210","journal-title":"J Bacteriol"},{"key":"BF00271553_CR35","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1146\/annurev.cb.02.110186.002435","volume":"2","author":"P Walter","year":"1986","unstructured":"Walter P, Lingappa VR (1986) Mechanisms of protein translocation across the endoplasmic reticulum membrane. Annu Rev Cell Biol 2:499\u2013516","journal-title":"Annu Rev Cell Biol"},{"key":"BF00271553_CR36","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1128\/jb.170.4.1622-1630.1988","volume":"170","author":"RA Welch","year":"1988","unstructured":"Welch RA, Pellett S (1988) The transcriptional organization of the Escherichia coli hemolysin genes. J Bacteriol 170:1622\u20131630","journal-title":"J Bacteriol"},{"key":"BF00271553_CR37","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1016\/S0021-9258(18)89669-7","volume":"260","author":"PB Wolfe","year":"1985","unstructured":"Wolfe PB, Rice M, Wickner W (1985) Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli. J Biol Chem 260:1836\u20131841","journal-title":"J Biol Chem"},{"key":"BF00271553_CR38","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1128\/jb.166.3.937-944.1986","volume":"166","author":"N Yanagida","year":"1986","unstructured":"Yanagida N, Uuzumi T, Beppu T (1986) Specific excretion of Serratia marcescens protease through the outer membrane of E. coli. J Bacteriol 166:937\u2013944","journal-title":"J Bacteriol"}],"container-title":["Molecular and General Genetics MGG"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/BF00271553.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/BF00271553\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/BF00271553","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/BF00271553.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,26]],"date-time":"2021-06-26T02:49:58Z","timestamp":1624675798000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/BF00271553"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,11]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,11]]}},"alternative-id":["BF00271553"],"URL":"https:\/\/doi.org\/10.1007\/bf00271553","relation":{},"ISSN":["0026-8925","1432-1874"],"issn-type":[{"value":"0026-8925","type":"print"},{"value":"1432-1874","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,11]]}}}