{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:33:22Z","timestamp":1790019202865,"version":"4.0.1"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"1-2","license":[{"start":{"date-parts":[[1987,6,1]],"date-time":"1987-06-01T00:00:00Z","timestamp":549504000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mole Gen Genet"],"published-print":{"date-parts":[[1987,6]]},"DOI":"10.1007\/bf00330426","type":"journal-article","created":{"date-parts":[[2004,10,24]],"date-time":"2004-10-24T00:59:11Z","timestamp":1098579551000},"page":"84-87","source":"Crossref","is-referenced-by-count":13,"title":["Cloning and expression of streptococcal pyrogenic exotoxin A and staphylococcal toxic shock syndrome toxin-1 in Bacillus subtilis"],"prefix":"10.1007","volume":"208","author":[{"given":"B. N.","family":"Kreiswirth","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. P.","family":"Handley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. M.","family":"Schlievert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. P.","family":"Novick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","reference":[{"key":"BF00330426_CR1","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1126\/science.318764","volume":"195","author":"WM Barnes","year":"1977","unstructured":"Barnes WM (1977) Plasmid detection and sizing in single colony lysates. Science 195:393\u2013394","journal-title":"Science"},{"key":"BF00330426_CR2","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1128\/IAI.22.3.681-688.1978","volume":"22","author":"EL Barsumian","year":"1978","unstructured":"Barsumian EL, Schlievert PM, Watson DW (1978) Nonspecific and specific immunological mitogenicity by group A streptococcal pyrogenic exotoxins. Infect Immun 22:681\u2013688","journal-title":"Infect Immun"},{"key":"BF00330426_CR3","first-page":"265","volume-title":"Microbial toxins, vol III","author":"MS Bergdoll","year":"1970","unstructured":"Bergdoll MS (1970) Enterotoxins. In: Montie T, Kadis S, Ajl SJ (eds) Microbial toxins, vol III. Academic Press, New York, pp 265\u2013326"},{"key":"BF00330426_CR4","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/0003-2697(84)90320-8","volume":"136","author":"MS Blake","year":"1984","unstructured":"Blake MS, Johnson KH, Russell-Jones GJ, Gotschlich EC (1984) A rapid, sensitive method for detection of alkaline phosphatase conjugated anti-antibody on western blots. Anal Biochem 136:175\u2013179","journal-title":"Anal Biochem"},{"key":"BF00330426_CR5","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1021\/bi00349a009","volume":"25","author":"DA Blomster-Hautamaa","year":"1986","unstructured":"Blomster-Hautamaa DA, Kreiswirth BN, Novick RP, Schlievert PM (1986a) Resolution of highly purified toxic shock syndrome toxin-1 into two distinct proteins by isoelectric focusing. Biochemistry 25:54\u201359","journal-title":"Biochemistry"},{"key":"BF00330426_CR6","doi-asserted-by":"crossref","first-page":"15783","DOI":"10.1016\/S0021-9258(18)66787-0","volume":"261","author":"DA Blomster-Hautamaa","year":"1986","unstructured":"Blomster-Hautamaa DA, Kreiswirth BN, Kornblum JS, Novick RP, Schlievert PM (1986b) The nucleotide and partial amino acid sequence of toxic shock syndrome toxin-1. J Biol Chem 261:15783\u201315786","journal-title":"J Biol Chem"},{"key":"BF00330426_CR7","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/BF00267940","volume":"168","author":"S Chang","year":"1979","unstructured":"Chang S, Cohen SN (1979) High frequency of transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet 168:111\u2013115","journal-title":"Mol Gen Genet"},{"key":"BF00330426_CR8","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1128\/JB.141.1.246-253.1980","volume":"141","author":"T Gryczan","year":"1980","unstructured":"Gryczan T, Shivakumar AG, Dubnau D (1980) Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J Bacteriol 141:246\u2013253","journal-title":"J Bacteriol"},{"key":"BF00330426_CR9","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1007\/BF00383496","volume":"194","author":"LP Johnson","year":"1984","unstructured":"Johnson LP, Schlievert PM (1984) Group A streptococcal phage T12 carries the structural gene for pyrogenic exotoxin type A. Mol Gen Genet 194:52\u201356","journal-title":"Mol Gen Genet"},{"key":"BF00330426_CR10","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1128\/jb.166.2.623-627.1986","volume":"166","author":"LP Johnson","year":"1986","unstructured":"Johnson LP, Tomai MA, Schlievert PM (1986) Bacteriophage involvement in group A streptococcal pyrogenic exotoxin A production. J Bacteriol 166:623\u2013627","journal-title":"J Bacteriol"},{"key":"BF00330426_CR11","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1084\/jem.131.3.611","volume":"131","author":"YB Kim","year":"1970","unstructured":"Kim YB, Watson DW (1970) A purified group A streptococcal pyrogenic exotoxin. Physicochemical and biological properties including the enhancement of susceptibility to endotoxin lethal shock. J Exp Med 131:611\u2013628","journal-title":"J Exp Med"},{"key":"BF00330426_CR12","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1038\/305709a0","volume":"305","author":"B Kreiswirth","year":"1983","unstructured":"Kreiswirth B, Lofdahl S, Betley M, O'Reilly M, Schlievert P, Bergdoll M, Novick RP (1983) The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature 305:709\u2013712","journal-title":"Nature"},{"key":"BF00330426_CR13","first-page":"17","volume-title":"Genetic engineering","author":"SR Kushner","year":"1978","unstructured":"Kushner SR (1978) An improved method for transformation of Escherichia coli with ColE1 derived plasmids. In: Boyer HB, Nicosia S (eds) Genetic engineering. Elsvier\/North-Holland, Amsterdam, pp 17"},{"key":"BF00330426_CR14","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"UK Laemmli","year":"1970","unstructured":"Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680\u2013685","journal-title":"Nature"},{"key":"BF00330426_CR15","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/0022-2836(70)90051-3","volume":"53","author":"M Mandel","year":"1970","unstructured":"Mandel M, Higa A (1970) Calcium-dependent bacteriophage DNA infection. J Mol Biol 53:159\u2013162","journal-title":"J Mol Biol"},{"key":"BF00330426_CR16","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/S0076-6879(80)65059-9","volume":"65","author":"AM Maxam","year":"1980","unstructured":"Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65:499\u2013560","journal-title":"Methods Enzymol"},{"key":"BF00330426_CR17","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1111\/j.1432-1033.1969.tb00754.x","volume":"11","author":"C Nauciel","year":"1969","unstructured":"Nauciel C, Blass B, Mangalo R (1969) Evidence for two molecular forms of streptococcal erythrogenic toxin. Conversion to a single form by 2-mercaptoethanol. Eur J Biochem 11:160\u2013164","journal-title":"Eur J Biochem"},{"key":"BF00330426_CR18","first-page":"81","volume":"15","author":"S Okubo","year":"1972","unstructured":"Okubo S, Yanagida T, Fujita DJ, Ohlsson-Wiilhelm BM (1972) The genetics of bacteriophage SPO1. Biken J 15:81\u201397","journal-title":"Biken J"},{"key":"BF00330426_CR19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/15569548509014412","volume":"4","author":"NJ Poindexter","year":"1985","unstructured":"Poindexter NJ, Schlievert PM (1985) The biochemical and immunological properties of toxic-shock syndrome toxin-1 (TSST-1) and association with TSS. J Toxicol Toxin Rev 4:1\u201339","journal-title":"J Toxicol Toxin Rev"},{"key":"BF00330426_CR20","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1128\/IAI.16.2.673-679.1977","volume":"16","author":"PM Schlievert","year":"1977","unstructured":"Schlievert PM, Bettin KM, Watson DW (1977) Purification and characterization of group A streptococcal pyrogenic exotoxin type C. Infect Immun 16:673\u2013679","journal-title":"Infect Immun"},{"key":"BF00330426_CR21","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1093\/infdis\/143.4.509","volume":"143","author":"PM Schlievert","year":"1981","unstructured":"Schlievert PM, Shands KN, Dan BB, Schmid GP, Nishimura RD (1981) Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic-shock syndrome. J Infect Dis 143:509\u2013516","journal-title":"J Infect Dis"},{"key":"BF00330426_CR22","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1093\/infdis\/96.1.14","volume":"96","author":"JH Schwab","year":"1955","unstructured":"Schwab JH, Watson DW, Cromartie WJ (1955) Further studies of group A streptococcal factors with lethal and cardiotoxic properties. J Infect Dis 96:14\u201318","journal-title":"J Infect Dis"},{"key":"BF00330426_CR23","doi-asserted-by":"crossref","first-page":"575","DOI":"10.4049\/jimmunol.115.2.575","volume":"115","author":"BD Smith","year":"1975","unstructured":"Smith BD, Johnson HM (1975) The effect of staphylococcal enterotoxins on the primary in vitro immune response. J Immunol 115:575\u2013578","journal-title":"J Immunol"},{"key":"BF00330426_CR24","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/S0076-6879(80)65048-4","volume":"65","author":"HO Smith","year":"1980","unstructured":"Smith HO (1980) Recovery of DNA from gels. Methods Enzymol 65:371\u2013380","journal-title":"Methods Enzymol"},{"key":"BF00330426_CR25","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1128\/JB.162.1.176-182.1985","volume":"162","author":"I Ulmanen","year":"1985","unstructured":"Ulmanen I, Lundstrom K, Lehtovaara P, Sarvas M, Ruohonen M, Palva I (1985) Transcription and translation of foreign genes in Bacillus subtilis by the aid of a secretion vector. J Bacteriol 162:176\u2013182","journal-title":"J Bacteriol"}],"container-title":["Molecular and General Genetics MGG"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/BF00330426.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/BF00330426\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/BF00330426","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/BF00330426.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T17:31:12Z","timestamp":1682789472000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/BF00330426"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,6]]},"references-count":25,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[1987,6]]}},"alternative-id":["BF00330426"],"URL":"https:\/\/doi.org\/10.1007\/bf00330426","relation":{},"ISSN":["0026-8925","1432-1874"],"issn-type":[{"value":"0026-8925","type":"print"},{"value":"1432-1874","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,6]]}}}