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The steroid sulphatase content in fibroblast, leukocyte and placental extracts correlates with the steroid sulphatase activity in these extracts. No steroid sulphatase protein was found in approximately 350 \u00b5g plasma proteins from a normal person. In three of four X\u2010linked ichthyosis patients a complete gene deletion was found by Southern hybridization with the full\u2010length STS cDNA as probe. Neither steroid sulphatase protein nor enzymatic activity was found in fibroblast extracts of these three patients. In a fibroblast extract of another X\u2010linked ichthyosis patient, which had a normal Southern blotting pattern, no immunoreactive protein was detected. Residual activity of steroid sulphatase was also not found after prolonged incubation of this fibroblast extract with the natural substrate oestrone sulphate.<\/jats:p>","DOI":"10.1007\/bf00711310","type":"journal-article","created":{"date-parts":[[2004,11,30]],"date-time":"2004-11-30T08:37:33Z","timestamp":1101803853000},"page":"17-26","source":"Crossref","is-referenced-by-count":3,"title":["Biochemical and immunological characterization of X\u2010linked ichthyosis"],"prefix":"10.1002","volume":"16","author":[{"given":"X.","family":"Fan","sequence":"first","affiliation":[{"name":"Institute of Medical Genetics Ernst\u2010Moritz\u2010Arndt University  Fleischmannstrasse 42\/44 Greifswald O\u20102200 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Petruschka","sequence":"additional","affiliation":[{"name":"Institute of Medical Genetics Ernst\u2010Moritz\u2010Arndt University  Fleischmannstrasse 42\/44 Greifswald O\u20102200 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Wulff","sequence":"additional","affiliation":[{"name":"Institute of Medical Genetics Ernst\u2010Moritz\u2010Arndt University  Fleischmannstrasse 42\/44 Greifswald O\u20102200 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"Grimm","sequence":"additional","affiliation":[{"name":"Institute of Medical Genetics Ernst\u2010Moritz\u2010Arndt University  Fleischmannstrasse 42\/44 Greifswald O\u20102200 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. 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