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Indeed, immunoblot analysis has become a valuable tool in the correct diagnosis and assignment of individual patients, except for X\u2010linked adrenoleukodystrophy (X\u2010ALD). We describe a simple immunoblotting procedure applicable to liver and fibroblast homo\u2010genates using antibodies raised against catalase and the three \u03b2\u2010oxidation enzyme proteins acyl\u2010CoA oxidase I, bifunctional protein and peroxisomal thiolase. The same procedure can also be used for chorionic villus biopsy specimens and has now become the method of choice for the prenatal diagnosis of Zellweger syndrome (and other disorders of peroxisome biogenesis) and rhizomelic chondrodysplasia punctata.<\/jats:p>","DOI":"10.1007\/bf00711433","type":"journal-article","created":{"date-parts":[[2004,11,30]],"date-time":"2004-11-30T10:35:54Z","timestamp":1101810954000},"page":"101-112","source":"Crossref","is-referenced-by-count":26,"title":["Immunoblot analysis of peroxisomal proteins in liver and fibroblasts from patients"],"prefix":"10.1002","volume":"18","author":[{"given":"R. J. A.","family":"Wanders","sequence":"first","affiliation":[{"name":"Academic Medical Centre, Departments of Pediatrics and Clinical Chemistry, Laboratory of Pediatric Clinical Chemistry, Section for Clinical Enzymology University Hospital Amsterdam  PO Box 22700 Meibergdreef 9 Amsterdam 1100 DE The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Dekker","sequence":"additional","affiliation":[{"name":"Academic Medical Centre, Departments of Pediatrics and Clinical Chemistry, Laboratory of Pediatric Clinical Chemistry, Section for Clinical Enzymology University Hospital Amsterdam  PO Box 22700 Meibergdreef 9 Amsterdam 1100 DE The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Ofman","sequence":"additional","affiliation":[{"name":"Academic Medical Centre, Departments of Pediatrics and Clinical Chemistry, Laboratory of Pediatric Clinical Chemistry, Section for Clinical Enzymology University Hospital Amsterdam  PO Box 22700 Meibergdreef 9 Amsterdam 1100 DE The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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