{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T02:50:11Z","timestamp":1771642211954,"version":"3.50.1"},"reference-count":25,"publisher":"Portland Press Ltd.","issue":"12","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1984,12,1]]},"abstract":"<jats:p>In the presence of 3-isobutyi-l-methylxanthine, VIP produced a dose-related (3 \u00d7 10\u22129 \u2212 10\u22127 M) increase (g-fold) in cAMP production in isolated HEp-2 cells incubated at 15\u00b0C in KRP buffer. Among the peptides structurally related to VIP, including secretin (10\u22127 M), pancreatic glucagon (10\u22126 M), PHI, somatostatin-14 (10\u22126 M), hpGRF (10\u22128 \u2212 4 \u00d7 10\u2212M), GIP (2 \u00d7 10\u22127 M), only PHI (3 \u00d7 10\u22127 M and above) is able to activate the cAMP-generating system in HEp-2 cells, but at 102 times lower potency. Under the same conditions, histamine (10\u22123 M) was also ineffective, while PGE 2 (10\u22127 \u2212 10\u22124 M) increased (0-fold) basal cAMP levels in HEp-2 cells. The VIP effect is related to the interaction os the peptide on VIP recognition sites (12SI-VIP-binding capacity), coupled to the membrane-bound adenylate cyclase. The results indicate that the transformed laryngeal cell line HEp-2 possessesa receptor-cAMP system preferentially activated by VIP (relative potencies: VIP &amp;gt; PHI \u226b other peptides of the secretin family), and suggest that this neuropeptide could modulate biological functions in normal laryngeal epithelia in man.<\/jats:p>","DOI":"10.1007\/bf01116697","type":"journal-article","created":{"date-parts":[[2005,2,6]],"date-time":"2005-02-06T09:32:36Z","timestamp":1107682356000},"page":"1045-1050","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Activation of the cAMP-generating system by vasoactive intestinal polypeptide (VIP) in the human laryngeal malignant cell line HEp-2"],"prefix":"10.1042","volume":"4","author":[{"given":"Annie","family":"Prost","sequence":"first","affiliation":[{"name":"INSERM U. 55, H\u00f4pital St. Antoine, 184 rue du Fg. 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