{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T16:54:33Z","timestamp":1694624073999},"reference-count":28,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,6,1]]},"abstract":"<jats:p>Calpain, a calcium-dependent, neutral cysteine-protease was purified from the erythrocyte cytosol of subjects having essential hypertension (HTN), sickle cell anaemia, (SCA), or kwashiorkor (KWA). Identical electrophoretic mobility on SDS-polyacrylamide gradient gel, sensitivity to micromolar amounts of Ca2+, absolute requirement for a reducing environment and a high susceptibility to inhibition by leupeptin and thiol-group modifying reagents confirm that calpain preparations from these erythrocytes are equivalent to calpain I. Whereas the extent of calpain activation of erythrocyte membrane Ca2+-pumping ATPase of normal subjects was almost equal to that due to calmodulin, calpain activation of the HTN and SCA pump was greater than activation by calmodulin. Like in normal membranes, exogenous calmodulin protected the Ca2+-pumping ATPase of these erythrocytes against calpainization; the degree of protection by calmodulin is least in SCA and HTN. Electrophoretic separation of erythrocyte membranes and the purified Ca2+-pumping ATPase of HTN, SCA and KWA subjects does not indicate the presence of fragments resulting from the proteolytic action of calpain.<\/jats:p>","DOI":"10.1007\/bf01117244","type":"journal-article","created":{"date-parts":[[2005,2,6]],"date-time":"2005-02-06T17:11:11Z","timestamp":1107709871000},"page":"281-291","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative action of calpain on erythrocyte Ca2+-pumping ATPase in sickle cell anaemia, essential hypertension and kwashiorkor"],"prefix":"10.1042","volume":"10","author":[{"given":"O. O.","family":"Olorunsogo","sequence":"first","affiliation":[{"name":"Laboratory for Biomembrane Research, Biochemistry Department, College of Medicine, University of Ibadan, Ibadan, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. O.","family":"Agbolade","sequence":"additional","affiliation":[{"name":"Laboratory for Biomembrane Research, Biochemistry Department, College of Medicine, University of Ibadan, Ibadan, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. O.","family":"Owojuyigbe","sequence":"additional","affiliation":[{"name":"Laboratory for Biomembrane Research, Biochemistry Department, College of Medicine, University of Ibadan, Ibadan, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A.","family":"Adebisi","sequence":"additional","affiliation":[{"name":"Laboratory for Biomembrane Research, Biochemistry Department, College of Medicine, University of Ibadan, Ibadan, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. O.","family":"Adebayo","sequence":"additional","affiliation":[{"name":"Laboratory for Biomembrane Research, Biochemistry Department, College of Medicine, University of Ibadan, Ibadan, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. G.","family":"Okunade","sequence":"additional","affiliation":[{"name":"Laboratory for Biomembrane Research, Biochemistry Department, College of Medicine, University of Ibadan, Ibadan, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","reference":[{"key":"CR1","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1113\/jphysiol.1961.sp006675","volume":"156","author":"E. T. Dunham","year":"1961","unstructured":"Dunham, E. T. and Glynn, I. M. (1961)J. Physiol. 156:274.","journal-title":"J. Physiol."},{"key":"CR2","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1007\/BF01901136","volume":"22","author":"H. J. Schatzman","year":"1966","unstructured":"Schatzman, H. J. (1966).Experentia 22:364.","journal-title":"Experentia"},{"key":"CR3","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/B978-0-12-152506-4.50013-3","volume":"6","author":"F. L. Bygrave","year":"1977","unstructured":"Bygrave, F. L. (1977).Curr. Top. Bioenerg. 6:259.","journal-title":"Curr. Top. Bioenerg."},{"key":"CR4","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev.bi.56.070187.002143","volume":"56","author":"E. Carafoli","year":"1987","unstructured":"Carafoli, E. (1987)Ann Rev. Biochem. 56:395.","journal-title":"Ann Rev. Biochem."},{"key":"CR5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1152\/physrev.1978.58.1.1","volume":"58","author":"M. Tada","year":"1978","unstructured":"Tada, M., Yamamato, T. and Tonomura, Y. (1978)Physiol. Rev. 58:1.","journal-title":"Physiol. Rev."},{"key":"CR6","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0005-2736(88)90359-8","volume":"945","author":"O. O. Olorunsogo","year":"1988","unstructured":"Olorunsogo, O. O., Villalobo, A., Wang, K. K. W. and Roufogalis B. D. (1988)Biochim. Biophys. Acta 945:33.","journal-title":"Biochim. Biophys. Acta"},{"key":"CR7","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0005-2736(86)90059-3","volume":"854","author":"A. Villalobo","year":"1986","unstructured":"Villalobo, A., Brown, L. and Roufogalis, B. D. (1986).Biochim. Biophys. Acta 854:9.","journal-title":"Biochim. Biophys. Acta"},{"key":"CR8","doi-asserted-by":"crossref","first-page":"4511","DOI":"10.1021\/bi00261a049","volume":"21","author":"E. Graf","year":"1982","unstructured":"Graf, E., Verma, A. K., Gorski, J. P., Laposchuck, G., Niggli, V., Zuruni, M., Carafoli, E. and Penniston, J. T. (1982)Biochem. 21:4511.","journal-title":"Biochem."},{"key":"CR9","doi-asserted-by":"crossref","first-page":"8588","DOI":"10.1016\/S0021-9258(19)68885-X","volume":"256","author":"V. Niggli","year":"1981","unstructured":"Niggli, V., Adunyah, E. S. and Carafoli, E. (1981)J. Biol. Chem. 256:8588.","journal-title":"J. Biol. Chem."},{"key":"CR10","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/S0021-9258(19)70149-5","volume":"256","author":"V. Niggli","year":"1981","unstructured":"Niggli, V., Adunyah, E. S., Penniston, J. T. and Carafoli, E. (1981)J. Biol. Chem. 256:395.","journal-title":"J. Biol. Chem."},{"key":"CR11","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1016\/S0006-291X(77)80107-1","volume":"77","author":"R. M. Gopinath","year":"1977","unstructured":"Gopinath, R. M. and Vincenzi, F. F. (1977)Biochem Biophys. Res. Commun. 77:1203.","journal-title":"Biochem Biophys. Res. Commun."},{"key":"CR12","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/0003-9861(88)90498-5","volume":"260","author":"K. K. W. Wang","year":"1988","unstructured":"Wang, K. K. W., Villalobo, A. and Roufogalis, B. D. (1988)Arch. Biophys. Biochem. 260:696.","journal-title":"Arch. Biophys. Biochem."},{"key":"CR13","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/0003-9861(88)90037-9","volume":"267","author":"K. K. W. Wang","year":"1988","unstructured":"Wang, K. K. W., Roufogalis, B. D. and Villalobo, A. (1988)Arch. Biophys. Biochem. 267:317.","journal-title":"Arch. Biophys. Biochem."},{"key":"CR14","first-page":"1365","volume":"89","author":"J. Palek","year":"1977","unstructured":"Palek, J. (1977)J. Lab. Clin. Med. 89:1365.","journal-title":"J. Lab. Clin. Med."},{"key":"CR15","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/246105a0","volume":"246","author":"J. W. Eaton","year":"1973","unstructured":"Eaton, J. W., Skelton, T. P., Swofford, H. S., Kolpiu, C. E. and Jacob, H. S. (1973)Nature 246:105.","journal-title":"Nature"},{"key":"CR16","doi-asserted-by":"crossref","unstructured":"Ziddek, W., Vetter, H., Zonnkley, H. and Loose, H. (1981)Clin. Sci. 61: (Suppl.) 41s.","DOI":"10.1042\/cs061041s"},{"key":"CR17","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/BF01114689","volume":"9","author":"O. O. Olorunsogo","year":"1989","unstructured":"Olorunsogo, O. O. (1989).Biosci. Rep. 9:359.","journal-title":"Biosci. Rep."},{"key":"CR18","volume-title":"Protein-energy Malnutrition","author":"G. A. O. Alleyne","year":"1978","unstructured":"Alleyne, G. A. O., Hay, R. W., Picou, D. I., Stanfield, J. P. and Whitehead R. G. (1978)Protein-energy Malnutrition. Edward Arnold Publishers. U.K. 243 pp."},{"key":"CR19","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1007\/BF01116952","volume":"5","author":"O. O. Olorunsogo","year":"1985","unstructured":"Olorunsogo, O. O., Okudolo, B. E., Lawal, S. O. A. and Falase, A. O. (1985)Biosci. Rep. 5:525.","journal-title":"Biosci. Rep."},{"key":"CR20","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","author":"O. H. Lowry","year":"1951","unstructured":"Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951)J. Biol. Chem. 193:265.","journal-title":"J. Biol. Chem."},{"key":"CR21","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/0006-291X(82)91772-7","volume":"106","author":"E. Melloni","year":"1982","unstructured":"Melloni, E., Sparatore, B., Salamino, F., Michetti, M. and Pontremoli, S. (1982)Biochem. Biophys. Res. Commun. 106:731.","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"CR22","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"U. K. Laemmli","year":"1970","unstructured":"Laemmli, U. K. (1970)Nature (Lond.) 227:680.","journal-title":"Nature (Lond.)"},{"key":"CR23","first-page":"159","volume-title":"Calcium and cell function","author":"S. Pontremoli","year":"1980","unstructured":"Pontremoli, S. and Melloni, E. (1980) In.Calcium and cell function (Cheung, W. Y., ed.) Vol.6 Academic Press, New York. pp. 159."},{"key":"CR24","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0143-4160(82)90010-0","volume":"3","author":"V. Niggli","year":"1982","unstructured":"Niggli, V., Adunyah, E. S., Cameron, B. F., Bababunmi, E. A. and Carafoli, E. (1982)Cell Calcium 3:131.","journal-title":"Cell Calcium"},{"key":"CR25","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1093\/clinchem\/23.9.1548","volume":"23","author":"H. P. Schwarz","year":"1977","unstructured":"Schwarz, H. P., Dahlke, N. B. and Dreisbach, L. (1977)Clin. Chem. 23:1548.","journal-title":"Clin. Chem."},{"key":"CR26","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1016\/0006-291X(72)90932-1","volume":"47","author":"S. E. Gordesky","year":"1972","unstructured":"Gordesky, S. E., Marinetti, G. V. and Sigel, G. B. (1972)Biochem. Biophys. Res. Comm. 47: 1004.","journal-title":"Biochem. Biophys. Res. Comm."},{"key":"CR27","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1042\/cs0630281","volume":"63","author":"S. N. Orlov","year":"1982","unstructured":"Orlov, S. N. and Postnov, Y. (1982)Clin. Sci. 63:281.","journal-title":"Clin. Sci."},{"key":"CR28","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1079\/BJN19710072","volume":"25","author":"W. A. Coward","year":"1971","unstructured":"Coward, W. A. (1971)Brit. J. Nutr. 25:145.","journal-title":"Brit. J. Nutr."}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/10\/3\/281\/468151\/bsr0100281.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/10\/3\/281\/468151\/bsr0100281.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T17:09:51Z","timestamp":1637082591000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/10\/3\/281\/51142\/Comparative-action-of-calpain-on-erythrocyte-Ca2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6,1]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,6,1]]}},"URL":"https:\/\/doi.org\/10.1007\/bf01117244","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6,1]]}}}