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The maximal effects on hormone release of TRH and db-cAMP, but not of TRH and MIX, were additive.<\/jats:p>\n               <jats:p>The Ca2+ channel blockers Co2+ (5 mM) and verapamil (100 \u03bcM) and the Ca2+ chelator EGTA (4 mM) abolished the stimulatory effect of TRH (1 \u03bcM) on hormone release. Co2+ and verapamil, but not EGTA, inhibited the stimulatory effect of db-cAMP (5 mM) on hormone release. The inhibitory effects of Co2+ and verapamil on GH release were counteracted by the combination of TRH and db-cAMP. For PRL release Co2+, but not verapamil, was able to inhibit the combined action of TRH and db-cAMP. Co2+, verapamil, and EGTA eliminated the stimulatory effect of MIX (1 mM) on PRL release while only Co2+ and EGTA affected the GH release. Hormone release in the presence of MIX plus verapamil or EGTA, but not Co2+, was increased by TRH.<\/jats:p>\n               <jats:p>The calmodulin antagonist trifluoperazine (TFP) at 30 \u03bcM inhibited basal hormone release and hormone release stimulated by TRH (1 \u03bcM), db-cAMP (5 mM), and MIX (1 mM). The Ca2+ ionophore A23187 (5 \u03bcM) had a stimulatory effect on basal hormone release which was abolished by 30 \u03bcM TFP.<\/jats:p>","DOI":"10.1007\/bf01121872","type":"journal-article","created":{"date-parts":[[2005,2,6]],"date-time":"2005-02-06T11:35:12Z","timestamp":1107689712000},"page":"93-105","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["On the involvement of cyclic AMP and extracellular Ca2+ in the regulation of hormone release from rat pituitary tumour (GH3) cells in culture"],"prefix":"10.1042","volume":"7","author":[{"given":"Kjersti","family":"Sletholt","sequence":"first","affiliation":[{"name":"Department of Physiology, The Norwegian College of Veterinary Medicine, PO Box 8146 Dep., 0033 Oslo 1, Norway"},{"name":"Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo 3, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Egil","family":"Haug","sequence":"additional","affiliation":[{"name":"Hormone Laboratory, Aker Hospital, 0514, Oslo 5 Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaare M.","family":"Gautvik","sequence":"additional","affiliation":[{"name":"Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo 3, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","reference":[{"key":"CR1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1210\/endo-116-1-73","volume":"116","author":"T. 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