{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:49:07Z","timestamp":1771627747327,"version":"3.50.1"},"reference-count":37,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1993,8,1]]},"abstract":"<jats:p>Calcitonin gene-related peptide (CGRP) shares about 46% and 20% amino acid sequence homology with islet amyloid polypeptide (IAPP) and salmon calcitonin (sCT). We investigated whether these related peptides could cross-react with the specific binding of 125I-[His]hCGRP I to the CGRP receptor in hamster insulinoma cell membranes. A rapid dissociation of membrane bound 125I-[His]hCGRP I could be induced in the presence of 1 \u03bcM chicken CGRP (cCGRP). The specific 125I-[His]hCGRP I binding was inhibited by the related peptides and their half-maximal inhibitory concentrations (IC50) were: cCGRP (0.1 nM), rat CGRP I and human CGRP I and II (1.0\u20132.0 nM), fragment of hCGRP I (8\u201337) (150 nM), human IAPP (440 nM). The non-amidated form of hIAPP; human diabetes-associated peptide (hDAP) did not inhibit the binding of 125I-[His]hCGRP I and sCT was only effective at a high concentration (1 \u03bcM). Binding of 125I-[His]hCGRP I was dose dependently inhibited by guanosine-5\u2032-O-(3-thiotriphosphate) or (GTP\u03b3S) and a 70% reduction of binding was obtained with 0.1 mM GTP\u03b3S. The IC50 value of cCGRP (0.1 nM) was increased 100-fold in the presence of 0.1 mM GTP\u03b3S. Human CGRP I and cCGRP at 2.5 \u03bcM did not stimulate the activity of hamster insulinoma cell membranes adenylate cyclase, while glucagon (1 \u03bcM) induced a 2-fold increase. Thus, specific CGRP receptors present in hamster \u03b2 cells are associated with G protein (s) and IAPP can interact with these receptors. These results and the observation that cCGRP and hCGRP I did not influence adenylate cyclase activity provide further evidence for CGRP receptor subtypes.<\/jats:p>","DOI":"10.1007\/bf01123504","type":"journal-article","created":{"date-parts":[[2005,2,6]],"date-time":"2005-02-06T12:24:09Z","timestamp":1107692649000},"page":"221-231","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Characterization of specific calcitonin gene related peptide receptors present in hamster pancreatic \u03b2 cells"],"prefix":"10.1042","volume":"13","author":[{"given":"A.","family":"Barakat","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U.55, H\u00f4pital Saint-Antoine, 75571 Paris Cedex 12, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Rosselin","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U.55, H\u00f4pital Saint-Antoine, 75571 Paris Cedex 12, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.-C.","family":"Marie","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U.55, H\u00f4pital Saint-Antoine, 75571 Paris Cedex 12, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","reference":[{"key":"CR1","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1038\/298240a0","volume":"298","author":"S. 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(1987) Regional distribution of calcitonin gene-related peptide and its specific binding sites in rats with particular reference to the nervous system.Neuroendocrinology,46:131?136.","journal-title":"Neuroendocrinology"},{"key":"CR4","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/0306-4522(85)90156-3","volume":"14","author":"P. K. Mulderry","year":"1985","unstructured":"Mulderry, P. K., Ghatei, M. A., Rodrigo, J., Allen, J. M. Rosenfeld, M. G., Polak, J. M. and Bloom, S. R. (1985) Calcitonin gene-related peptide in cardiovascular tissues of the rat.Neuroscience,14:947?954.","journal-title":"Neuroscience"},{"key":"CR5","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1210\/endo-119-2-865","volume":"119","author":"M. Pettersson","year":"1986","unstructured":"Pettersson, M., Ahr\u00e9n, B., B\u00f6ttcher, G., and Sundler, F. 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