{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T04:16:55Z","timestamp":1778991415078,"version":"3.51.4"},"reference-count":23,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1993,6,1]]},"abstract":"<jats:p>We report the effects of the tetracycline analogues 4-dedimethylaminotetracycline (CMT-1) and minocycline on osteoclast spreading and motility. Both agents influenced the morphometric descriptor of cell spread area, \u03c1, producting cellular retraction or an R effect (half-times: 30 and 44 minutes for CMT-1 and minocycline, respectively). At the concentrations employed, the tetracycline-induced R effects were significantly slower than, but were qualitatively similar to, those resulting from Ca2+ \u201creceptor\u201d activation through the application of 15 mM-[Ca2+] (slopes: \u22121.25, \u22120.18, and \u22124.40\/minute for 10 mg\/l-[CMT-1], 10 mg\/l-[minocycline] and 15 mM-[Ca2+], respectively). In contrast, the same tetracycline concentrations did not influence osteoclast margin ruffling activity as described by \u03bc, a motility descriptor known to be influenced by elevations of cellular cyclic AMP. Thus, the tetracyclines exert morphometric effects comparable to changes selectively activated by occupancy of the osteoclast Ca2+ \u201creceptor\u201d which may act through an increase in cytosolic [Ca2+].<\/jats:p>","DOI":"10.1007\/bf01149962","type":"journal-article","created":{"date-parts":[[2005,2,16]],"date-time":"2005-02-16T12:56:17Z","timestamp":1108558577000},"page":"175-182","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The effect of tetracyclines on quantitative measures of osteoclast morphology"],"prefix":"10.1042","volume":"13","author":[{"given":"Mone","family":"Zaidi","sequence":"first","affiliation":[{"name":"The Bone Research Unit, Division of Biochemical Medicine, St. George's Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baljit S.","family":"Moonga","sequence":"additional","affiliation":[{"name":"The Bone Research Unit, Division of Biochemical Medicine, St. George's Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher L.-H.","family":"Huang","sequence":"additional","affiliation":[{"name":"The Physiological Laboratory, Cambridge University, Cambridge CB2 3EG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.S.M. 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