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The folate receptor cross-reacted with antibodies against human milk folate binding protein, e.g. the syncytothrophoblastic layer of molar placenta tissue sections showed strongly positive immunostaining. The gel filtration profile contained two radioligand-bound peaks (25 and 100 kDa), however, with considerable overlap. Only a single band of 70 kDa was seen on SDS-PAGE immunoblotting. The folate receptor in placental tissue may play a crucial role in the transfer of folate from maternal circulation to the fetus.<\/jats:p>","DOI":"10.1007\/bf01207263","type":"journal-article","created":{"date-parts":[[2005,2,25]],"date-time":"2005-02-25T17:48:26Z","timestamp":1109353706000},"page":"379-389","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Characterization of the folate receptor in human molar placenta"],"prefix":"10.1042","volume":"16","author":[{"given":"Jan","family":"Holm","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry, Horsens Hospital, Horsens, DK-8700, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steen Ingemann","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Central Hospital, Hiller\u00f8d, DK-3400, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl W.","family":"Nichols","sequence":"additional","affiliation":[{"name":"Department of Pathology, West Paces Medical Center, 3200 Howell Mill Rd., NW Atlanta, Georgia 30327, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mimi","family":"H\u00f8ier-Madsen","sequence":"additional","affiliation":[{"name":"Laboratory for Autoimmune Serology, State Serum Institute, Copenhagen, DK-2300 S, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poul Erik","family":"Helkj\u00e6r","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Central Hospital, N\u00e6stved, DK-4700, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","reference":[{"key":"CR1","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1126\/science.1310359","volume":"255","author":"R. 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