{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:54:58Z","timestamp":1761512098374},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1990,5,1]],"date-time":"1990-05-01T00:00:00Z","timestamp":641520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World j. surg."],"published-print":{"date-parts":[[1990,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Thyroid stimulating hormone (TSH) and epidermal growth factor (EGF) are growth factors for some thyroid cells in cultures. We have previously found more EGF receptors in neoplastic human thyroid tissues than in normal thyroid tissues. We have also found a higher TSH\u2010stimulated adenylate cyclase (AC) activity in neoplastic human thyroid tissues than in normal thyroid tissues. To clarify the relationship between the effect of EGF and TSH on thyroid tissue, we measured the binding of EGF and TSH and the basal, TSH\u2010stimulated and forskolin\u2010stimulated adenylate cyclase activity in 49\u00a0normal, hyperplastic and neoplastic human thyroid tissues (5\u00a0normal, 2 Hashimoto thyroiditis, 5 Graves' disease, 14\u00a0multinodular goiters, 9 follicular adenomas, S follicular carcinomas, 8 papillary carcinomas, and 1 undifferentiated carcinoma). Specific binding of EGF and TSH were measured by radioreceptor assays using competitive inhibition of radio\u2010labeled ligand by unlabeled ligand. Basal, maximally (300\u00a0mU\/ml) TSH\u2010stimulated, and maximally (100\u00a0mM) forskolin\u2010stimulated adenylate cyclase activities were also measured in the same membrane particulate fractions from the thyroid tissues. We found: neoplastic thyroid tissues bind more labeled EGF than nonneoplastic thyroid tissues; follicular adenomas and carcinomas have higher EGF binding than other thyroid tissues; a weak but significant correlation between specific EGF binding and specific TSH binding, and between specific EGF binding and TSH\u2010stimulated adenylate cyclase activity of the thyroid membrane preparations. These findings are consistent with the hypothesis that TSH stimulates an increase in thyroid EGF receptors by increasing intracellular cAMP. The higher binding of EGF and the higher TSH\u2010stimulated AC activity may explain why thyroid neoplasms grow to a larger size than normal thyroid tissues<\/jats:bold>.<\/jats:p>","DOI":"10.1007\/bf01658542","type":"journal-article","created":{"date-parts":[[2005,5,8]],"date-time":"2005-05-08T13:03:12Z","timestamp":1115557392000},"page":"410-417","source":"Crossref","is-referenced-by-count":12,"title":["Epidermal growth factor receptors and adenylate cyclase activity in human thyroid tissues"],"prefix":"10.1002","volume":"14","author":[{"given":"Quan\u2010Yang","family":"Duh","sequence":"first","affiliation":[{"name":"Veterans Administration Medical Center Surgical Service mail route 112, 4150 Clement Street 94121 San Francisco California USA"},{"name":"Department of Surgery University of California San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allan E.","family":"Siperstein","sequence":"additional","affiliation":[{"name":"Veterans 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