{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:07:41Z","timestamp":1760548061307},"reference-count":8,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[1989,10,2]],"date-time":"1989-10-02T00:00:00Z","timestamp":623289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Inher Metab Disea"],"published-print":{"date-parts":[[1990,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Two distinct pyrimidine 5\u2032\u2010nucleotidases (UMPH\u20101 and UMPH\u20102) have previously been detected in human erythrocytes; UMPH\u20101 is deficient in a haemolytic anaemia, while UMPH\u20102 is unaffected. Only the erythrocyte shows pathological effects in this disorder. Here we have studied lymphoblastoid cell lines from control and UMPH\u20101 deficient patients to determine whether UMPH\u20101 can be detected in lymphoblastoid cells and whether the deficiency of UMPH\u20101 results in any measurable metabolic effects. Both UMPH\u20101 and a UMPH\u20102\u2010like activity were found to be present in control lymphoblastoid cells. UMPH\u20101 was undetectable in the patients' cells; minor but significant changes were found in the pyrimidine nucleotide and nucleoside pools in the cells.<\/jats:p>","DOI":"10.1007\/bf01799571","type":"journal-article","created":{"date-parts":[[2005,6,21]],"date-time":"2005-06-21T16:28:46Z","timestamp":1119371326000},"page":"701-706","source":"Crossref","is-referenced-by-count":5,"title":["Pyrimidine 5\u2032\u2010nucleotidase activity in normal and deficient human lymphoblastoid cells"],"prefix":"10.1002","volume":"13","author":[{"given":"D. A.","family":"Hopkinson","sequence":"first","affiliation":[{"name":"MRC Human Biochemical Genetics Unit University College  London WC1 UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. M.","family":"Swallow","sequence":"additional","affiliation":[{"name":"MRC Human Biochemical Genetics Unit University College  London WC1 UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Marinaki","sequence":"additional","affiliation":[{"name":"Department of Chemical Pathology University of Cape Town  Cape Town 7925 South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. H.","family":"Harley","sequence":"additional","affiliation":[{"name":"Department of Chemical Pathology University of Cape Town  Cape Town 7925 South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1989,10,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113707"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V56.2.251.251"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.2.588"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-1809.1983.tb00965.x"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-1809.1983.tb00966.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107826"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.4.3.238"}],"container-title":["Journal of Inherited Metabolic Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/BF01799571","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/BF01799571","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T04:48:38Z","timestamp":1694926118000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1007\/BF01799571"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,10,2]]},"references-count":8,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1990,9]]}},"alternative-id":["10.1007\/BF01799571"],"URL":"https:\/\/doi.org\/10.1007\/bf01799571","archive":["Portico"],"relation":{},"ISSN":["0141-8955","1573-2665"],"issn-type":[{"value":"0141-8955","type":"print"},{"value":"1573-2665","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,10,2]]}}}