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In an attempt to identify proteins which mediate phosphate transport, we treated renal BBM vesicles prepared from mutant male (<jats:italic>Hyp<\/jats:italic>\/Y) and normal male (+\/Y) littermates, with radiolabelled<jats:italic>N<\/jats:italic>\u2010ethylmaleimide (NEM), in the presence or absence of arsenate which is a competitive inhibitor of phosphate transport. Polyacrylamide gel electrophoresis revealed labelling of membrane proteins in the 40\u201345 kDa range; addition of arsenate during NEM treatment inhibited labelling. These findings indicate a 40\u201345 kDa protein as a component of the renal BBM phosphate transport system(s). We found no difference between protein labelling of the renal BBM from<jats:italic>Hyp<\/jats:italic>\/Y and +\/Y mice.<\/jats:p>","DOI":"10.1007\/bf01800070","type":"journal-article","created":{"date-parts":[[2005,6,21]],"date-time":"2005-06-21T16:59:49Z","timestamp":1119373189000},"page":"243-252","source":"Crossref","is-referenced-by-count":2,"title":["Effect of the X\u2010linked<i>Hyp<\/i> mutation on<i>N<\/i>\u2010ethylmaleimide labelling of proteins in renal brush border membrane"],"prefix":"10.1002","volume":"10","author":[{"given":"E. J.","family":"Vizel","sequence":"first","affiliation":[{"name":"Medical Research Council Genetics Group, Center for Human Genetics and Department of Biology, deBelle Laboratory for Biochemical Genetics McGill University\u2010Montreal Children's Hospital Research Institute  2300 Tupper Street Montreal Quebec H3H 1P3 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. S.","family":"Tenenhouse","sequence":"additional","affiliation":[{"name":"Medical Research Council Genetics Group, Center for Human Genetics and Department of Biology, deBelle Laboratory for Biochemical Genetics McGill University\u2010Montreal Children's Hospital Research Institute  2300 Tupper Street Montreal Quebec H3H 1P3 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. R.","family":"Scriver","sequence":"additional","affiliation":[{"name":"Medical Research Council Genetics Group, Center for Human Genetics and Department of Biology, deBelle Laboratory for Biochemical Genetics McGill University\u2010Montreal Children's Hospital Research Institute  2300 Tupper Street Montreal Quebec H3H 1P3 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1986,7,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00657156"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1420575"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1590395"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.12.4667"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(61)90902-7"},{"key":"e_1_2_1_7_1","first-page":"891","article-title":"Specific labelling and partial purification of the M protein, a component of the \u03b2\u2010galactoside transport system ofEscherichia Coli","volume":"54","author":"Fox C. 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