{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:04:22Z","timestamp":1760645062830},"reference-count":5,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1990,3,21]],"date-time":"1990-03-21T00:00:00Z","timestamp":637977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Inher Metab Disea"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>This paper describes a non\u2010invasive method for the study of mitochondrial morphology in cultured human skin fibroblasts by confocal scanning laser microscopy after staining the mitochondria with 2\u2010[4\u2010(dimethylaminostyryl]\u20101\u2010methylpyridinium iodide. This method is applied to compare mitochondria in fibroblasts from healthy individuals and from patients with mitochondrial myopathy. In most cases there is a striking swelling of the patient's mitochondria and a loss of fine structure. The results with respect to the potential of this method as a diagnostic tool are discussed.<\/jats:p>","DOI":"10.1007\/bf01804387","type":"journal-article","created":{"date-parts":[[2005,6,27]],"date-time":"2005-06-27T23:58:36Z","timestamp":1119916716000},"page":"45-48","source":"Crossref","is-referenced-by-count":9,"title":["Confocal scanning laser microscopy of mitochondria: a possible tool in the diagnosis of mitochondrial disorders"],"prefix":"10.1002","volume":"14","author":[{"given":"M. H. J.","family":"Ruiters","sequence":"first","affiliation":[{"name":"Laboratory of Physiological Chemistry University of Groningen  Bloemsingel 10 Groningen 9712 KZ The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. A.","family":"van Spronsen","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, Section of Molecular Cytology University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O. H.","family":"Skjeldal","sequence":"additional","affiliation":[{"name":"Centre for Mental Retardation, Department of Paediatrics, Rikshospitalet University of Oslo  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Stromme","sequence":"additional","affiliation":[{"name":"Centre for Mental Retardation, Department of Paediatrics, Rikshospitalet University of Oslo  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. R.","family":"Scholte","sequence":"additional","affiliation":[{"name":"Department of Biochemistry I Erasmus University  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Pzyrembel","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Sophia's Children's Hospital Erasmus University  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. P. A.","family":"Smit","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University of Groningen  Groningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Ruitenbeek","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University of Nijmegen  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Agsteribbe","sequence":"additional","affiliation":[{"name":"Laboratory of Physiological Chemistry University of Groningen  Bloemsingel 10 Groningen 9712 KZ The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1990,3,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(76)90096-7"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.290010306"},{"key":"e_1_2_1_4_1","first-page":"748","article-title":"Three\u2010dimensional chromatin distribution in neuroblastoma nuclei shown by confocal scanning laser microscopy","volume":"337","author":"Brakenhoff G. J.","year":"1989","journal-title":"Nature"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01812848"},{"key":"e_1_2_1_6_1","first-page":"563","article-title":"Studies on the formation of lactate and pyruvate from glucose in cultured skin fibroblasts: implications for detection of respiratory chain defects","volume":"19","author":"Wijburg F. A.","year":"1989","journal-title":"Biochem. Int."}],"container-title":["Journal of Inherited Metabolic Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/BF01804387","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T05:28:14Z","timestamp":1694928494000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1007\/BF01804387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,3,21]]},"references-count":5,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,1]]}},"alternative-id":["10.1007\/BF01804387"],"URL":"https:\/\/doi.org\/10.1007\/bf01804387","archive":["Portico"],"relation":{},"ISSN":["0141-8955","1573-2665"],"issn-type":[{"value":"0141-8955","type":"print"},{"value":"1573-2665","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,3,21]]}}}