{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T21:39:09Z","timestamp":1778708349185,"version":"3.51.4"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[1979,9,19]],"date-time":"1979-09-19T00:00:00Z","timestamp":306547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Inher Metab Disea"],"published-print":{"date-parts":[[1979,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rabbit antiserum was prepared against purified normal human liver phenylalanine hydroxylase. This was used to test for cross\u2010reacting material in crude extracts of livers from patients with classical phenylketonuria (PKU); the samples from patients were two livers obtained at autopsy and a needle biopsy core. None of these enzymically inactive livers contained detectable cross\u2010reacting material capable of neutralizing antibody activity in double immunodiffusion and\/or enzyme inhibition experiments. In an earlier study, no phenylalanine hydroxylase was found in the two autopsy PKU livers by an affinity chromatography method or by the use of a specific antiserum raised against purified monkey liver phenylalanine hydroxylase (Choo<jats:italic>et al.<\/jats:italic>, 1979a). This evidence suggests that mutations in the PKU patients studied may be regulatory in nature.<\/jats:p>","DOI":"10.1007\/bf01805662","type":"journal-article","created":{"date-parts":[[2005,6,27]],"date-time":"2005-06-27T23:52:00Z","timestamp":1119916320000},"page":"79-84","source":"Crossref","is-referenced-by-count":8,"title":["Observations indicating the nature of the mutation in phenylketonuria"],"prefix":"10.1002","volume":"2","author":[{"given":"K. 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