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Enriched cultures contain between 60 and 75% multinucleated cells and may be maintained in culture without fetal calf serum for up to 14 days without significant overgrowth by the few contaminating mononuclear parental cells. Complementation appears not to occur between GM<jats:sub>1<\/jats:sub> gangliosidosis and mucopolysaccharidosis, type VIB (Morquio) cell strains; this experimental observation provides support for the earlier hypothesis that the mutations for these conditions are allelic. Earlier observations that complementation does not occur between selected phenotypic variants (viz., neuronopathic forms and those without neurological involvement) of sphingomyelin storage (Niemann\u2010Pick) disease or Gaucher's disease are confirmed.<\/jats:p>","DOI":"10.1007\/bf01819286","type":"journal-article","created":{"date-parts":[[2005,6,27]],"date-time":"2005-06-27T17:57:26Z","timestamp":1119895046000},"page":"95-99","source":"Crossref","is-referenced-by-count":6,"title":["A method for enrichment of hybrid somatic cells: Complementation studies in certain lysosomal enzymopathies"],"prefix":"10.1002","volume":"8","author":[{"given":"P. V.","family":"Nelson","sequence":"first","affiliation":[{"name":"Department of Chemical Pathology The Adelaide Children's Hospital  72 King William Street North Adelaide South Australia 5006 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. F.","family":"Carey","sequence":"additional","affiliation":[{"name":"Department of Chemical Pathology The Adelaide Children's Hospital  72 King William Street North Adelaide South Australia 5006 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1984,11,28]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00291589"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.9.5588"},{"key":"e_1_2_1_4_1","first-page":"831","volume-title":"The Metabolic Basis of Inherited Disease","author":"Brady R. 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