{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T05:07:35Z","timestamp":1770700055958,"version":"3.49.0"},"reference-count":32,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,2,1]]},"abstract":"<jats:p>The question as to whether the homologous peptides CGRP and IAPP can regulate insulin secretion in RINm5F cells was addressed. Chicken CGRP displayed a reproducible inhibitory effect on insulin secretion within 0.1 and 1 nM concentrations and a stimulatory effect at higher concentrations. The maximal stimulatory effects on insulin secretion were obtained with 1.0 \u03bcM of chicken CGRP (cCGRP), human \u03b1-CGRP (h \u03b1-CGRP) and human IAPP (hIAPP) which caused 246 \u00b1 22, 302 \u00b1 63 and 224 \u00b1 14 percent increases of control levels, respectively (p &amp;lt; 0.001). Similarly, maximal accumulations of cAMP were obtained with 1.0 \u03bcM of cCGRP, h \u03b1-CGRP and hIAPP with the respective percent increases of control levels of 587 \u00b1 24, 436 \u00b1 41 and 410 \u00b1 25 (p &amp;lt; 0.005). Thus the stimulatory effects on insulin secretion in RINm5F cells by cCGRP, h \u03b1-CGRP and hIAPP appear to be mediated by the cAMP pathway. Chicken CGRP, the most potent peptide tested, displayed a correlated dose response stimulation of intracellular cAMP and insulin release within the concentration range of 10\u20131000nM. The EC50 values of cCGRP for cAMP accumulation and insulin release were similar (20nM and 10 nM respectively). The stimulatory effect of IAPP on cAMP was not additive with that of cCGRP suggesting that IAPP action was mediated by CGRP receptors. This hypothesis was further sustained by a preferential inhibition of125I[His]h \u03b1-CGRP binding to RINm5F cells by cCGRP as compared to IAPP.<\/jats:p>\n               <jats:p>We conclude that CGRP and IAPP, through a direct action on a chicken CGRP preferring receptor present in \u03b2 cells, stimulated insulin by a cAMP mediated pathway.<\/jats:p>","DOI":"10.1007\/bf01901633","type":"journal-article","created":{"date-parts":[[2005,7,29]],"date-time":"2005-07-29T16:20:40Z","timestamp":1122654040000},"page":"1-13","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Calcitonin gene-related peptide and islet amyloid polypeptide stimulate insulin secretion in RINm5F cells through a common receptor coupled to a generation of cAMP"],"prefix":"10.1042","volume":"14","author":[{"given":"A.","family":"Barakat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Skoglund","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Boissard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Rosselin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.-C.","family":"Marie","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U.55, H\u00f4pital Saint-Antoine, 75571 Paris Cedex 12, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","reference":[{"key":"BF01901633_CR1","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1038\/298240a0","volume":"298","author":"S. G. Amara","year":"1982","unstructured":"Amara, S. G., Jonas, V., Rosenfeld, M. G., Ong, E. S. and Evans, R. M. (1982) Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products.Nature,298:240\u2013244.","journal-title":"Nature"},{"key":"BF01901633_CR2","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1038\/304129a0","volume":"304","author":"M. G. Rosenfeld","year":"1983","unstructured":"Rosenfeld, M. G., Mermod, J. J., Amara, S. G., Swanson, L. W., Sawchenko, P. E., Rivier, J., Vale, W. W. and Evans, R. M. (1983) Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing.Nature,304:129\u2013135.","journal-title":"Nature"},{"key":"BF01901633_CR3","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1210\/endo-119-2-865","volume":"119","author":"M. Pettersson","year":"1986","unstructured":"Pettersson, M., Ahr\u00e9n, B., B\u00f6ttcher, G. and Sundler, F. (1986) Calcitonin gene-related peptide: Occurrence in pancreatic islets in the mouse and the rat and inhibition of insulin secretion in the mouse.Endocrinology,119:865\u2013869.","journal-title":"Endocrinology"},{"key":"BF01901633_CR4","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/BF00353900","volume":"269","author":"C. Sternini","year":"1992","unstructured":"Sternini, C., De Giorgio, R., Anderson, K., Watt, P. C., Brunicardi, F. C., Widdison, A. L., Wong, H., Reber, H. A., Walsh, J. H. and Go, V. L. W. (1992). Species differences in the immunoreactive patterns of calcitonin gene-related peptide in the pancreas.Cell Tissue Res.,269:447\u2013458.","journal-title":"Cell Tissue Res."},{"key":"BF01901633_CR5","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/BF00401195","volume":"35","author":"P. Westermark","year":"1992","unstructured":"Westermark, P., Johnson, K. H., O'Brien, T. D., and Betsholtz, C. 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Pept."},{"key":"BF01901633_CR10","doi-asserted-by":"crossref","first-page":"168","DOI":"10.2337\/diab.39.2.168","volume":"39","author":"A. Yamaguchi","year":"1990","unstructured":"Yamaguchi, A., Chiba, T., Morishita, T., Nakamura, A., Inui, T., Yamatani, T., Kadowaiki, S., Chihara, K., Fukase, M. and Fujita, T. (1990) Calcitonin gene-related peptide and induction of hyperglycemia in concious rats in vivo.Diabetes,39:168\u2013174.","journal-title":"Diabetes"},{"key":"BF01901633_CR11","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/0006-291X(90)90636-2","volume":"167","author":"H-C. Fehmann","year":"1990","unstructured":"Fehmann, H-C., Weber, V., G\u00f6ke, R., G\u00f6ke, B., Eissele, R. and Arnold, R. (1990) Islet amyloid polypeptide (IAPP; Amylin) influences the endocrine but not the exocrine rat pancreas.Biochem. Biophys. Res. Commun.,167:1102\u20131108.","journal-title":"Biochem. Biophys. Res. 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(1990) Direct interaction of islet amyloid polypeptide (IAPP) with calcitonin gene-related peptide (CGRP) receptors present in b cell membrane.Diabetologia,33:P399.","journal-title":"Diabetologia"},{"key":"BF01901633_CR17","first-page":"395","volume":"56","author":"H. L. Cailla","year":"1973","unstructured":"Cailla, H. L., Racine-weisbugh, M. S. and Delaage, N. A. (1973) Adenosine 3\u20325\u2032-cyclic monophsphate assay at 10.5 mole level.Anal. Biochem.,56:395\u2013407.","journal-title":"Anal. Biochem."},{"key":"BF01901633_CR18","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/0163-7258(92)90036-Y","volume":"56","author":"D. R. Poyner","year":"1992","unstructured":"Poyner, D. R. (1992) Calcitonin gene-related peptide: Multiple actions, multiple receptors.Pharmac. Ther.,56:23\u201351.","journal-title":"Pharmac. Ther."},{"key":"BF01901633_CR19","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1016\/0024-3205(92)90106-Y","volume":"51","author":"B. J.-A. 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Seifert","year":"1986","unstructured":"Seifert, H., Sawchenko, P., Chesnut, J., Rivier, J., Vale, W. and Pandol, S. J. (1986) Receptor for calcitonin gene-related peptide: Binding to exocrine pancreas mediates biological actions.Am J. Physiol,249:G147\u2013G151.","journal-title":"Am J. Physiol"},{"key":"BF01901633_CR25","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.1210\/endo-123-5-2591","volume":"123","author":"A. Yamaguchi","year":"1988","unstructured":"Yamaguchi, A., Chiba, T., Yamatani, T., Inui, T., Morishita, T., Nakamura, A., Kadowaski, S., Fukase, M. and Fujita, T. (1988) Calcitonin gene-related peptide (CGR) stimulates adenylate cyclase activation via a guanine nucleotide-dependent process in rat liver plasma membranes.Endocrinology,123:2591\u20132596.","journal-title":"Endocrinology"},{"key":"BF01901633_CR26","unstructured":"Barakat, A., Marie, J.-C., and Rosselin, G. (1990) Receptors for calcitonin gene-related peptide (CGRP) on insulinoma membranes.C.R. Acad. 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(1988) Biological properties of two models of calcitonin gene-related peptide with idealized amphiphilic a helices of different lengths.Biochemistry.,27:7600\u20137607.","journal-title":"Biochemistry."},{"key":"BF01901633_CR29","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1055\/s-2007-1009315","volume":"21","author":"K. Morita","year":"1989","unstructured":"Morita, K., Kato, I., Uzawa, T., Hori, M. and Noda, T. (1989) Structure-activity relationship of calcitonin gene-related peptide.Horm, metabol. Res.,21:666\u2013668.","journal-title":"Horm, metabol. Res."},{"key":"BF01901633_CR30","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1126\/science.2983422","volume":"227","author":"D. Goltzman","year":"1985","unstructured":"Goltzman, D. and Mitchell, J. 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Ther.,254:123\u2013128.","journal-title":"J. Pharmac. Exp. Ther."}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/14\/1\/1\/468881\/bsr0140001.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/14\/1\/1\/468881\/bsr0140001.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T17:43:27Z","timestamp":1637084607000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/14\/1\/1\/52195\/Calcitonin-gene-related-peptide-and-islet-amyloid"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2,1]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,2,1]]}},"URL":"https:\/\/doi.org\/10.1007\/bf01901633","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2,1]]}}}