{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:40:32Z","timestamp":1760920832943,"version":"build-2065373602"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[1973,7,1]],"date-time":"1973-07-01T00:00:00Z","timestamp":110332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[1973,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Phospholipase has been prepared in a stable, partially purified form from the small intestine of mice infected with the tapeworm<jats:italic>Hymenolepis nana<\/jats:italic>. The enzyme(s) attacks diacylphosphatides with liberation of free fatty acids and a corresponding decrease in phospholipid phosphorus, without accumulation of lysophosphatides. The reactivity with various substrates is strongly influenced by their physical state, the presence of other lipids, proteins or detergents. The phospholipids of some biomembranes (mitochondria, microsomes, red cell ghosts) are readily hydrolyzed under customary reaction conditions. The ensuing biochemical, morphological and functional alterations have been documented. In contrast, the diacylphospholipids of the cell membrane (intact erythrocytes,<jats:italic>Pseudomonas aerunginosa<\/jats:italic>) are not accessible to phospholipase action unless some alteration of the integrity of the cell is induced by physical or chemical means (hemolysis, polymyxin). The enzyme is proposed as a tool for the investigation of biomembranes and as a model for the study of phospholipase activity.<\/jats:p>","DOI":"10.1007\/bf02531718","type":"journal-article","created":{"date-parts":[[2006,11,21]],"date-time":"2006-11-21T06:24:02Z","timestamp":1164090242000},"page":"415-425","source":"Crossref","is-referenced-by-count":19,"title":["Preparation and characterization of mouse intestinal phospholipase"],"prefix":"10.1002","volume":"8","author":[{"given":"Athos","family":"Ottolenghi","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology Duke Medical Center Durham North Carolina 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1973,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.41.070172.001021"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1874-6047(08)60087-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(71)90275-X"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0710615"},{"key":"e_1_2_1_6_1","first-page":"123","volume":"74","author":"Marples E.A.","year":"1960","journal-title":"Ibid."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00911a012"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)40180-4"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(65)90017-2"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02532741"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1180233"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02531719"},{"key":"e_1_2_1_13_1","first-page":"87","volume-title":"Phospholipids","author":"Ansell G.B.","year":"1964"},{"key":"e_1_2_1_14_1","unstructured":"Ansell G.B. andS.N.Hawthorne Ibid. p.99."},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0740123"},{"key":"e_1_2_1_16_1","first-page":"423","volume-title":"Methods in Enzymology","author":"Fleischer S.","year":"1967"},{"key":"e_1_2_1_17_1","unstructured":"Fleischer S. andB.Fleischer Ibid. p.412."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1139\/y59-099"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)84756-1"},{"key":"e_1_2_1_20_1","first-page":"466","volume":"234","author":"Barlett G.R.","year":"1959","journal-title":"Ibid."},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI103259"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(69)90052-9"},{"key":"e_1_2_1_23_1","first-page":"566","volume-title":"Methods in Enzymology","author":"Skipski V.P.","year":"1966"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)49224-1"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.2307\/3571142"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0840497"},{"key":"e_1_2_1_27_1","first-page":"427","volume-title":"Methods in Enzymology","author":"Fleischer S.","year":"1967"},{"key":"e_1_2_1_28_1","first-page":"214","volume-title":"Methods in Enzymology","author":"Wood W.A.","year":"1955"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.39.070170.003021"},{"key":"e_1_2_1_30_1","first-page":"171","volume-title":"Recent Advances in Microbiology","author":"Kaback H.R.","year":"1970"},{"key":"e_1_2_1_31_1","first-page":"35","volume-title":"Current Topics in Membranes and Transport","author":"Kaback H.R.","year":"1970"},{"key":"e_1_2_1_32_1","first-page":"421","volume-title":"The Molecular Basis of Membrane Transport","author":"Kaback H.R.","year":"1969"},{"key":"e_1_2_1_33_1","first-page":"2305","volume":"23","author":"Fleischer S.","year":"1964","journal-title":"Fed. 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