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A mixture of synthetic conjugated bile acids has been separated by reverse\u2010phase systems consisting of either a Waters Associates' \u201cfatty\u2010acid analysis\u201d or a \u03bcBondapak\/C<jats:sub>18<\/jats:sub> column eluted with a mixture of 2\u2010propanol\/potassium phosphate buffer (pH 2.5 or 7.0). The major conjugated bile acids present in the gallbladder bile of obese subjects have been analyzed each in less than 30 min and quantitated with a U.V. detector set at 193 nm. Some of the 5\u03b1\u2010 and 5\u03b2\u2010isomers of conjugated bile salts could be resolved in straight\u2010phase systems on Corasil II or \u03bcPorasil columns. Mass spectra of the conjugated bile acids obtained by electron impact were characteristic of the type of amino acid attached to the side chain, and the number of hydroxyl substituents on the nucleus. Most of the isomers could readily be differentiated by the relative intensities of the fragment ions.<\/jats:p>","DOI":"10.1007\/bf02533858","type":"journal-article","created":{"date-parts":[[2006,11,22]],"date-time":"2006-11-22T03:24:01Z","timestamp":1164165841000},"page":"971-975","source":"Crossref","is-referenced-by-count":40,"title":["Bile acids LVII. Analysis of bile acids by high pressure liquid chromatography and mass spectrometry"],"prefix":"10.1002","volume":"13","author":[{"given":"Roger","family":"Shaw","sequence":"first","affiliation":[{"name":"Edward A. Doisy Department of Biochemistry St. Louis University School of Medicine St. Lousi MO 63104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William H.","family":"Elliott","sequence":"additional","affiliation":[{"name":"Edward A. 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