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At a calmodulin antagonist concentration of 25 \u03bcmol, the incorporation of [<jats:sup>14<\/jats:sup>C] mevalonolactone into cellular cholesterol was suppressed to about 30% (hepatoma cells) and 10% (human skin fibroblasts) of control values. When the total nonsaponifiable [<jats:sup>14<\/jats:sup>C] lipids were separated and analyzed by two\u2010dimensional thin layer chromatography, an accumulation of [<jats:sup>14<\/jats:sup>C] desmosterol was observed along with reduced formation of [<jats:sup>14<\/jats:sup>C] cholesterol. However, when cells were preincubated in the presence of [<jats:sup>14<\/jats:sup>C] dihydrolanosterol, [<jats:sup>14<\/jats:sup>C] cholesterol formation was not inhibited by the calmodulin antagonists. About 25% of the cell\u2010associated dihydrolanosterol radioactivity was converted to cholesterol in both control and calmodulin antagonist\u2010pretreated cells. The data suggest that calmodulin antagonists prevent the conversion of desmosterol into cholesterol by inhibiting sterol \u0394<jats:sup>24<\/jats:sup> reductase and that the enzymes catalyzing sterol ring modifications are not affected by the inhibitors.<\/jats:p>","DOI":"10.1007\/bf02533989","type":"journal-article","created":{"date-parts":[[2006,11,22]],"date-time":"2006-11-22T04:16:21Z","timestamp":1164168981000},"page":"261-265","source":"Crossref","is-referenced-by-count":9,"title":["Calmodulin antagonists suppress cholesterol synthesis by inhibiting sterol \u0394<sup>24<\/sup> reductasereductase"],"prefix":"10.1002","volume":"22","author":[{"given":"Ivan","family":"Filipovic","sequence":"first","affiliation":[{"name":"Institute of Physiological Chemistry University of Muenster Waldeyerstrasse 15 Muenster D\u201044OO Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eckhart","family":"Buddecke","sequence":"additional","affiliation":[{"name":"Institute of Physiological Chemistry University of Muenster Waldeyerstrasse 15 Muenster D\u201044OO Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1987,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)38120-6"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00294a001"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42859-6"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)86479-7"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)81322-2"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(86)90325-5"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(77)90061-3"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1320439"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)38931-8"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(69)15022-3"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(69)15023-5"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1830309"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.36.070167.003355"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)39825-3"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(84)90006-2"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)81176-0"}],"container-title":["Lipids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/BF02533989","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:40:08Z","timestamp":1760874008000},"score":1,"resource":{"primary":{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/10.1007\/BF02533989"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,4]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1987,4]]}},"alternative-id":["10.1007\/BF02533989"],"URL":"https:\/\/doi.org\/10.1007\/bf02533989","archive":["Portico"],"relation":{},"ISSN":["0024-4201","1558-9307"],"issn-type":[{"type":"print","value":"0024-4201"},{"type":"electronic","value":"1558-9307"}],"subject":[],"published":{"date-parts":[[1987,4]]}}}