{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:58:47Z","timestamp":1761512327096,"version":"build-2065373602"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[1984,9,1]],"date-time":"1984-09-01T00:00:00Z","timestamp":462844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[1984,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The triacylglycerols of very low density lipoproteins (VLDL\u2010TG) were analyzed in samples from normal subjects and patients with Frederickson\u2019s Type III and Type IV hyperlipoproteinemia. VLDL were obtained by conventional ultracentrifugation, and the triacylglycerols were isolated by thin\u2010layer chromatography (TLC). Representative sn\u20101,2(2,3)\u2010 and sn\u20101,3\u2010diacylglycerols were generated by Grignard degradation of the triacylglycerols, and were resolved by TLC on borate\u2010treated silica gel. The molecular association of the fatty acids in the diacylglycerol moieties was determined by gasliquid chromatography with mass spectrometry (GC\/MS) of the tertiary\u2010butyldimethylsilyl ethers. The positional distribution of the fatty acids was established by the Brockerhoff stereospecific analysis. The results showed a marked asymmetry in the distribution of the fatty acids in all samples, with the saturated acids predominantly in the sn\u20101\u2010position and the unsaturated fatty acids distributed about equally between the sn\u20102\u2010 and sn\u20103\u2010positions. In all instances, the molecular species composition of the sn\u20101,2\u2010, sn\u20102,3\u2010 and sn\u20101,3\u2010diacylglycerols was found to be similar to that calculated for 1\u2010random 2\u2010random 3\u2010random distribution of triacylglycerols. There were marked differences in the quantitative composition of the molecular species of the VLDL\u2010TG between normal subjects and patients, but these discrepancies were attributed to differences in the fatty acid composition of the samples.<\/jats:p>","DOI":"10.1007\/bf02534528","type":"journal-article","created":{"date-parts":[[2006,11,29]],"date-time":"2006-11-29T00:12:41Z","timestamp":1164759161000},"page":"683-691","source":"Crossref","is-referenced-by-count":17,"title":["Studies of triacyglycerol structure of very low density lipoproteins of normolipemic subjects and patients with type III and type IV hyperlipoproteinemia"],"prefix":"10.1002","volume":"19","author":[{"given":"J. J.","family":"Myher","sequence":"first","affiliation":[{"name":"Banting and Best Department of Medical Research University of Toronto Toronto M5G 1L6 Canada"},{"name":"Department of Medicine University of Toronto Toronto M5G 1L6 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Kuksis","sequence":"additional","affiliation":[{"name":"Banting and Best Department of Medical Research University of Toronto Toronto M5G 1L6 Canada"},{"name":"Department of Medicine University of Toronto Toronto M5G 1L6 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. C.","family":"Breckenridge","sequence":"additional","affiliation":[{"name":"Banting and Best Department of Medical Research University of Toronto Toronto M5G 1L6 Canada"},{"name":"Department of Medicine University of Toronto Toronto M5G 1L6 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A.","family":"Little","sequence":"additional","affiliation":[{"name":"Banting and Best Department of Medical Research University of Toronto Toronto M5G 1L6 Canada"},{"name":"Department of Medicine University of Toronto Toronto M5G 1L6 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1984,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.1.6.406"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1139\/o73-200"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02532622"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(76)90369-7"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02532700"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02533457"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02533264"},{"volume-title":"Lipid and Lipoprotein Analysis","year":"1974","author":"Lipid Research Clinics Program","key":"e_1_2_1_9_1"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4831-9942-9.50008-5"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)81198-6"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02531179"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(66)90143-3"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02631865"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)39632-2"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)39039-8"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02640489"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(74)90380-7"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac50026a006"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1139\/o78-095"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(69)90078-2"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1973.tb03028.x"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02532550"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.50.1.102"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02530965"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(70)90060-3"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.2.1.44"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02534275"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)38102-5"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1139\/o76-021"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(84)90193-0"}],"container-title":["Lipids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/BF02534528","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:45:41Z","timestamp":1760881541000},"score":1,"resource":{"primary":{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/10.1007\/BF02534528"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,9]]},"references-count":32,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1984,9]]}},"alternative-id":["10.1007\/BF02534528"],"URL":"https:\/\/doi.org\/10.1007\/bf02534528","archive":["Portico"],"relation":{},"ISSN":["0024-4201","1558-9307"],"issn-type":[{"type":"print","value":"0024-4201"},{"type":"electronic","value":"1558-9307"}],"subject":[],"published":{"date-parts":[[1984,9]]}}}