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One hundred microliters of deproteinated serum were extracted with chloroform and injected on a reverse\u2010phase column. Separation occurred within 16 min for all compounds of interest employing a mobile solvent of MeOH\/AcN\/CHCl<jats:sub>3<\/jats:sub> (47\u223647\u22366). All compounds were quantified at the wavelengths cited by integrated peak areas using retinyl acetate as a daily standard. Analysis of serum from a hypercarotenemic anorexia nervosa patient and a person suffering from hypervitaminosis A are presented as examples of the clinical application of this procedure.<\/jats:p>","DOI":"10.1007\/bf02534557","type":"journal-article","created":{"date-parts":[[2006,12,5]],"date-time":"2006-12-05T03:06:27Z","timestamp":1165287987000},"page":"253-258","source":"Crossref","is-referenced-by-count":43,"title":["Determination of lycopene, \u03b1\u2010 and \u03b2\u2010carotene and retinyl esters in human serum by reversed\u2010phase high performance liquid chromatography"],"prefix":"10.1002","volume":"18","author":[{"given":"Carla R.","family":"Broich","sequence":"first","affiliation":[{"name":"Department of Food Science University of Illinois Urbana IL 61801"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard E.","family":"Gerber","sequence":"additional","affiliation":[{"name":"Department of Food Science University of Illinois Urbana IL 61801"},{"name":"Department of Food Science and Technology, Nutrition and Dietetics University of Rhode Island Kingston RI 02881"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"John W.","family":"Erdman","sequence":"additional","affiliation":[{"name":"Department of Food Science University of Illinois Urbana IL 61801"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1983,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)84952-3"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)81715-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/32.10.2143"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)92091-0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/15.6.479"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/9780470110331.ch1","volume":"15","author":"McLaren D.S.","year":"1967","journal-title":"Methods Biochem. 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