{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:43:02Z","timestamp":1760920982698,"version":"build-2065373602"},"reference-count":16,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[1987,11,1]],"date-time":"1987-11-01T00:00:00Z","timestamp":562723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[1987,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effects of 2\u2010lysophosphatidylcholine (2\u2010LPC), the alkyl lysophospholipid derivatives (ALP) 1\u2010<jats:italic>O<\/jats:italic>\u2010octadecyl\u20102\u2010<jats:italic>O<\/jats:italic>\u2010methyl\u2010<jats:italic>rac<\/jats:italic>\u2010glycero\u20103\u2010phosphocholine (ET\u201018\u2010OCH<jats:sub>3<\/jats:sub>) and 1\u2010<jats:italic>O<\/jats:italic>\u2010hexadecyl\u2010<jats:italic>sn<\/jats:italic>\u2010glycero\u20103\u2010phospho\u2010trimethyl\u2010ammonio\u2010hexanol, the 2\u2010acetamide analog of platelet\u2010activating factor (PAF) 1\u2010<jats:italic>O<\/jats:italic>\u2010octadecyl\u20102\u2010acetamide\u2010<jats:italic>sn<\/jats:italic>\u2010glycero\u20103\u2010phosphocholine, the thioether lysophospholipid derivative (TLP) BM 41.440 and the ether\u2010linked lipoidal amine CP\u201046,665 on tritiated thymidine uptake and trypan blue dye exclusion were tested in vitro in various freshly explanted cell samples from human nonneoplastic bone marrow and human leukemias. In both assay systems, a dose range of 1\u201320 \u03bcg\/ml of the compounds was tested after 24, 48 and 72 hr of coincubation with the cells.<\/jats:p><jats:p>The trypan blue dye exclusion revealed statistically significant preferential cytotoxicity in leukemic cells for three compounds with the order of quantitative selectiveness: ET\u201018\u2010OCH<jats:sub>3<\/jats:sub>&gt;BM41.440&gt;2\u2010acetamide analog of PAF. CP\u201046,665 was the most toxic compound, but did not reveal significant differences between nonneoplastic bone marrow and leukemic cells when added in concentrations greater than 1 \u03bcg\/ml. The trimethyl\u2010ammoniohexanol compound showed only minor activity in the majority of tests, when added at concentrations &lt;20 \u03bcg\/ml. 2\u2010LPC was rather ineffective.<\/jats:p><jats:p>The tritiated thymidine uptake showed only preferential antiproliferative effects towards leukemic cells of ET\u201018\u2010OCH<jats:sub>3<\/jats:sub> and, sometimes, within the dose time frame tested of BM 41.440. All compounds tested except 2\u2010LPC and the trimethyl\u2010ammonio\u2010hexanol compound were active also in this assay (inhibition of uptake&gt;50% of the controls). Based on these results, ET\u201018\u2010OCH<jats:sub>3<\/jats:sub> and BM 41.440 are recommended for experimental bone marrow purging.<\/jats:p>","DOI":"10.1007\/bf02535552","type":"journal-article","created":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T10:25:40Z","timestamp":1164968740000},"page":"904-910","source":"Crossref","is-referenced-by-count":20,"title":["Cytotoxic effects of ether lipids and derivatives in human nonneoplastic bone marrow cells and leukemic cells in vitro"],"prefix":"10.1002","volume":"22","author":[{"given":"Hans D.","family":"Schick","sequence":"first","affiliation":[{"name":"Division of Hematology and Oncology Department of Medicine III, University Munich Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang E.","family":"Berdel","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology Department of Medicine III, University Munich Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Fromm","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology Department of Medicine III, University Munich Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Fink","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology Department of Medicine III, University Munich Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Jehn","sequence":"additional","affiliation":[{"name":"Department of Medical Statistics and Epidemiology Technische Universitael Munich Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kurt","family":"Ulm","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Biophysical Chemistry Goettingen Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anneliese","family":"Reichert","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology Department of Medicine III, University Munich Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hansj\u00f6rg","family":"Eibl","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology Department of Medicine, University Goettingen Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clemens","family":"Unger","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Department of Medicine I Technische Universitaet Ismaninger Str 22 Munich 80 8000 Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johann","family":"Rastetter","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology Department of Medicine III, University Munich Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1987,11]]},"reference":[{"key":"e_1_2_2_2_1","first-page":"41","volume-title":"Phospholipids and Cellular Regulation","author":"Berdel W.E.","year":"1985"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V64.6.1288.1288"},{"key":"e_1_2_2_4_1","first-page":"569","volume":"12","author":"Vogler W.R.","year":"1984","journal-title":"Exp. Hematol."},{"key":"e_1_2_2_5_1","first-page":"1206","volume":"45","author":"Berdel W.E.","year":"1985","journal-title":"Cancer Res."},{"key":"e_1_2_2_6_1","first-page":"5538","volume":"43","author":"Berdel W.E.","year":"1983","journal-title":"Cancer Res."},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.19840960404"},{"key":"e_1_2_2_8_1","first-page":"770","volume":"43","author":"Long R.C.","year":"1983","journal-title":"Cancer Res."},{"key":"e_1_2_2_9_1","unstructured":"8.Aglietta M. Piacibello W. Sanavio F. andGavosto F.(1982)3rd International Symposium on Therapy of Acute Leukemias(December 1982 Rome) p.175."},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00320725"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V63.3.545.545"},{"key":"e_1_2_2_12_1","first-page":"3211","volume":"41","author":"Honma Y.","year":"1981","journal-title":"Cancer Res."},{"key":"e_1_2_2_13_1","first-page":"441","volume-title":"Augmenting Agents in Cancer Therapy","author":"Munder P.G.","year":"1981"},{"key":"e_1_2_2_14_1","first-page":"3894","volume":"38","author":"Andreesen R.","year":"1978","journal-title":"Cancer Res."},{"key":"e_1_2_2_15_1","first-page":"541","volume":"43","author":"Berdel W.E.","year":"1983","journal-title":"Cancer Res."},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02579858"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02579854"}],"container-title":["Lipids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/BF02535552","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T14:09:12Z","timestamp":1760882952000},"score":1,"resource":{"primary":{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/10.1007\/BF02535552"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,11]]},"references-count":16,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1987,11]]}},"alternative-id":["10.1007\/BF02535552"],"URL":"https:\/\/doi.org\/10.1007\/bf02535552","archive":["Portico"],"relation":{},"ISSN":["0024-4201","1558-9307"],"issn-type":[{"type":"print","value":"0024-4201"},{"type":"electronic","value":"1558-9307"}],"subject":[],"published":{"date-parts":[[1987,11]]}}}