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In contrast, progesterone, cortexolone, 17\u2010\u03b2\u2010estradiol, testosterone, estriol, aldosterone, corticosterone, dexamethasone, dehydroepiandrosterone, 11\u2010\u03b2\u2010hydroxyandrosterone, 11\u2010ketoaetiocholanolone, epiaetiocholanolone and 5\u2010\u03b2\u2010pregnane\u20103\u03b1,20\u03b1\u2010diol, provoked no significant changes in the uptake of the exogenous acid. Of all the steroids tested, only 11\u2010dexycorticosterone, dexamethasone and 17\u2010\u03b2\u2010estradiol evoked a significant inhibition on the arachidonate biosynthesis in both kinds of cells. Testosterone, estriol, aldosterone and corticosterone provoked a significant inhibition of \u03945\u2010desaturase in HTC cells. In dexamethasone, this effect was dose\u2010dependent (0 to 10<jats:sup>\u22124<\/jats:sup> M). Simultaneous incubation with 17\u2010\u03b2\u2010 estradiol or 11\u2010deoxycorticosterone with dexamethasone led to an extent of inhibition on arachidonate biosynthesis that did not surpass the effect of each drug. Pretreatment of isolated hepatocytes with the antiglucocorticoid, cortexolone, prevented the dexamethasone\u2010induced inhibition of arachidonate biosynthesis. Normal rat liver microsomes preincubated in vitro with dexamethasone, 11\u2010deoxycorticosterone, 17\u2010\u03b2\u2010estradiol, corticosterone or estriol (10<jats:sup>\u22126<\/jats:sup> or 10<jats:sup>\u22124<\/jats:sup> M concentration), showed no significant changes in the \u03945\u2010desaturase activity. The results obtained suggest that the effect of the steroids on arachidonic acid biosynthesis in normal hepatocytes and HTC cells requires receptor occupancy and probably is mediated through a common biochemical mechanism.<\/jats:p>","DOI":"10.1007\/bf02535651","type":"journal-article","created":{"date-parts":[[2006,11,29]],"date-time":"2006-11-29T02:09:38Z","timestamp":1164766178000},"page":"1053-1058","source":"Crossref","is-referenced-by-count":27,"title":["Effect of various steroids on the biosynthesis of arachidonic acid in isolated hepatocytes and HTC cells"],"prefix":"10.1002","volume":"23","author":[{"given":"Carlos A.","family":"Marra","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Bioquimicas de la Plata (INIBIOLP) CONICET\u2010UNLP, Facultad de Ciencias M\u00e9dicas calles 60 y 120 La Plata 60 y 120 (1900) Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda J. 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