{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:26:15Z","timestamp":1789986375466,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[1994,10,1]],"date-time":"1994-10-01T00:00:00Z","timestamp":780969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of glucose stimulation (25 mM for 5 min) on the phospholipid and neutral lipid composition of isolated pancreatic islets was studied to find out whether there is a change in the mass of potential lipid mediators or modulators of insulin secretion. For comparison, the lipid compositions of homogenates and subcellular fractions from RINm5F insulin\u2010secreting tumor cells and of glucose\u2010stimulated streptozotocin\/nicotinamide\u2010induced islet cell tumors were analyzed. After separation of the lipid extract into a neutral and an acidic fraction by anion\u2010exchange chromatography, lipids were separated by high\u2010performance thin\u2010layer chromatography and quantitated by<jats:italic>in situ<\/jats:italic> densitometry of the cupric sulfate\u2010charred bands. In glucose\u2010stimulated islets, the molar percentages of phosphatidic acid (PA) and of phosphatidylinositol were significantly increased (3.1 vs. 4.7 mol% and 8.6 vs. 11.8 mol%), while those of all other phospholipids and neutral lipids, including 1,2\u2010diacylglycerol, were not significantly changed. In stimulated islet cell tumors, an increase of PA was visible in the microsomal fraction, and there was an increase of lysophosphatidylcholine in the mitochondrial fraction. However, in both tumoral tissues, particularly in RINm5F cells, the lipid distribution pattern showed abnormalities which can be regarded as a loss of differentiation and which limit the usefulness of these tissues for the study of the physiological regulation of lipid metabolism during glucose stimulation. In conclusion, the data are in accordance with a role of PA early in stimulus\u2010secretion coupling. The well\u2010known stimulation of phospholipid synthesis in pancreatic islets during glucose\u2010induced insulin secretion does not result in an increase in the total phospholipid mass.<\/jats:p>","DOI":"10.1007\/bf02538912","type":"journal-article","created":{"date-parts":[[2006,11,29]],"date-time":"2006-11-29T14:09:11Z","timestamp":1164809351000},"page":"685-692","source":"Crossref","is-referenced-by-count":56,"title":["Lipid composition of glucose\u2010stimulated pancreatic islets and insulin\u2010secreting tumor cells"],"prefix":"10.1002","volume":"29","author":[{"given":"Ingo","family":"Rustenbeck","sequence":"first","affiliation":[{"name":"Institute of Pharmacology and Toxicology University of G\u00f6ttingen Robert\u2010Koch\u2010Str. 40 G\u00f6ttingen D\u201037075 Germany"},{"name":"Institute of Clinical Biochemistry Hannover Medical School Hannover D\u201030623 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Matthies","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology University of G\u00f6ttingen Robert\u2010Koch\u2010Str. 40 G\u00f6ttingen D\u201037075 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigurd","family":"Lenzen","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology University of G\u00f6ttingen Robert\u2010Koch\u2010Str. 40 G\u00f6ttingen D\u201037075 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1994,10]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7827(87)90010-5"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.40.12.1565"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(72)80505-2"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1975.tb02448.x"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1520661"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1520667"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2270995"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(86)90212-X"},{"key":"e_1_2_2_10_1","first-page":"153","volume":"10","author":"Montague W.","year":"1980","journal-title":"Horm. Metab. Res. Suppl."},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(82)80650-9"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2230467"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/306067a0"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/308693a0"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)61389-4"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(90)91730-G"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(92)90007-F"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)35676-4"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(82)90036-7"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2700427"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(84)90134-6"},{"key":"e_1_2_2_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(86)90232-8"},{"key":"e_1_2_2_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02535277"},{"key":"e_1_2_2_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1979.236.4.E391"},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1965.tb34819.x"},{"key":"e_1_2_2_26_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2120439"},{"key":"e_1_2_2_27_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-137-35561"},{"key":"e_1_2_2_28_1","doi-asserted-by":"publisher","DOI":"10.1530\/acta.0.1090361"},{"key":"e_1_2_2_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(78)90046-5"},{"key":"e_1_2_2_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(86)90179-X"},{"key":"e_1_2_2_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(82)83012-7"},{"key":"e_1_2_2_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(85)90447-6"},{"key":"e_1_2_2_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/01483918308066881"},{"key":"e_1_2_2_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)83381-2"},{"key":"e_1_2_2_35_1","first-page":"207","volume":"2","author":"Rustenbeck I.","year":"1989","journal-title":"J. Planar Chromatogr."},{"key":"e_1_2_2_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(77)90011-2"},{"key":"e_1_2_2_37_1","first-page":"60","volume-title":"Techniques of Lipidology","author":"Kates M.","year":"1986"},{"key":"e_1_2_2_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(85)90002-4"},{"key":"e_1_2_2_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(90)90036-N"},{"key":"e_1_2_2_40_1","first-page":"129","volume-title":"Phospholipids","author":"Barenholz Y.","year":"1982"},{"key":"e_1_2_2_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)81159-3"},{"key":"e_1_2_2_42_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-118-4-1498"},{"key":"e_1_2_2_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)91157-X"},{"key":"e_1_2_2_44_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2810163"},{"key":"e_1_2_2_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(91)90470-P"},{"key":"e_1_2_2_46_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.29.9.686"},{"key":"e_1_2_2_47_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114463"},{"key":"e_1_2_2_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(89)90206-3"},{"key":"e_1_2_2_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(88)90082-5"},{"key":"e_1_2_2_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01230690"},{"key":"e_1_2_2_51_1","first-page":"559","volume":"70","author":"Upreti G.","year":"1983","journal-title":"J. Natl. Cancer Inst."},{"key":"e_1_2_2_52_1","first-page":"567","volume":"70","author":"Upreti G.","year":"1983","journal-title":"J. Natl. Cancer Inst."},{"key":"e_1_2_2_53_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-114-3-1012"},{"key":"e_1_2_2_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(88)90138-5"}],"container-title":["Lipids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/BF02538912","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T09:03:49Z","timestamp":1760951029000},"score":1,"resource":{"primary":{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/10.1007\/BF02538912"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10]]},"references-count":53,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1994,10]]}},"alternative-id":["10.1007\/BF02538912"],"URL":"https:\/\/doi.org\/10.1007\/bf02538912","archive":["Portico"],"relation":{},"ISSN":["0024-4201","1558-9307"],"issn-type":[{"value":"0024-4201","type":"print"},{"value":"1558-9307","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10]]}}}