{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:11:45Z","timestamp":1791101505723,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[1994,10,1]],"date-time":"1994-10-01T00:00:00Z","timestamp":780969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A procedure for the preparation of specific molecular species of<jats:sc>d<\/jats:sc>\u2010<jats:italic>erythro<\/jats:italic>\u2010lactosylceramide involving deacylation and reacylation of lactosylceramide prepared from bovine brain gangliosides is described. Lactosylceramide was<jats:italic>N<\/jats:italic>\u2010deacylated by alkaline hydrolysis and the resulting four lysolactosylceramides, which contained d18\u22361, d20\u22361, d18\u22360 and d20\u22360 long\u2010chain bases, were simultaneously re\u2010<jats:italic>N<\/jats:italic>\u2010acylated with the<jats:italic>N<\/jats:italic>\u2010succinimidyl ester of either 16\u22360, 18\u22360, 20\u22360, 24\u22360, 20\u22361, 22\u22361 or 24\u22361 fatty acid. The resulting lactosylceramide contained four molecular species of lactosylceramides, i.e., d18\u22361, d20\u22361, d18\u22360 and d20\u22360 long\u2010chain bases coupled with the fatty acid that was introduced. Lactosylceramides prepared in this manner were separated into four individual molecular species by high\u2010performance liquid chromatography (HPLC). Each of the purified molecular species of lactosylceramide was quantitated by HPLC after derivatization with benzoylchloride and was characterized by mass spectrometry. The yields of reacylated lactosylceramide were 38\u201358% relative to the starting lactosylceramide; the purity of each of the molecular species of lactosylceramide was greater than 95%.<\/jats:p>","DOI":"10.1007\/bf02538917","type":"journal-article","created":{"date-parts":[[2006,11,29]],"date-time":"2006-11-29T14:09:11Z","timestamp":1164809351000},"page":"721-725","source":"Crossref","is-referenced-by-count":5,"title":["Preparation of defined molecular species of lactosylceramide by chemical deacylation and reacylation with<i>N<\/i>\u2010succinimidyl fatty acid esters"],"prefix":"10.1002","volume":"29","author":[{"given":"Hiroko","family":"Kadowaki","sequence":"first","affiliation":[{"name":"Department of Medicine Boston University School of Medicine Boston Massachusetts"},{"name":"Division of Lipid Metabolism Boston Veterans Administration Medical Center Boston Massachusetts 02130"},{"name":"Boston VA Medical Center 150 South Huntington Avenue Research Building, Room RB\u20108 Boston MA 02130"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne A.","family":"Grant","sequence":"additional","affiliation":[{"name":"Department of Medicine Boston University School of Medicine Boston Massachusetts"},{"name":"Division of Lipid Metabolism Boston Veterans Administration Medical Center Boston Massachusetts 02130"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1994,10]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)41583-4"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(74)90234-3"},{"key":"e_1_2_2_4_1","first-page":"4997","volume":"43","author":"Kannagi R.","year":"1983","journal-title":"Cancer Res."},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00371a035"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00091a017"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)38225-0"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)39677-2"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)36555-9"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90178-5"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)38927-6"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(82)83012-7"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)37723-3"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_2_15_1","first-page":"33","volume-title":"Handbook of Lipid Research: Sphingolipid Biochemistry","author":"Hakomori S\u2010I.","year":"1983"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)38567-9"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02533114"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00323a042"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)41686-4"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1971.tb11997.x"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32944-7"},{"key":"e_1_2_2_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a129378"},{"key":"e_1_2_2_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)34395-9"},{"key":"e_1_2_2_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(91)90021-9"},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)41424-5"},{"key":"e_1_2_2_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)39355-X"},{"key":"e_1_2_2_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)38353-X"}],"container-title":["Lipids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/BF02538917","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T09:03:54Z","timestamp":1760951034000},"score":1,"resource":{"primary":{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/10.1007\/BF02538917"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10]]},"references-count":26,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1994,10]]}},"alternative-id":["10.1007\/BF02538917"],"URL":"https:\/\/doi.org\/10.1007\/bf02538917","archive":["Portico"],"relation":{},"ISSN":["0024-4201","1558-9307"],"issn-type":[{"value":"0024-4201","type":"print"},{"value":"1558-9307","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10]]}}}