{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T09:06:31Z","timestamp":1695891991091},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[1989,9,1]],"date-time":"1989-09-01T00:00:00Z","timestamp":620611200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["In Vitro Cell Dev Biol"],"published-print":{"date-parts":[[1989,9]]},"DOI":"10.1007\/bf02623668","type":"journal-article","created":{"date-parts":[[2007,5,3]],"date-time":"2007-05-03T18:29:39Z","timestamp":1178216979000},"page":"839-848","source":"Crossref","is-referenced-by-count":14,"title":["Improved maintenance of adult rat hepatocytes in a new serum-free medium in the presence or absence of barbiturates"],"prefix":"10.1007","volume":"25","author":[{"given":"Masahiro","family":"Miyazaki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasunori","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Munehiro","family":"Oda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Kawai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyan","family":"Bai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiro","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"key":"BF02623668_CR1","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/0016-5085(78)90522-X","volume":"75","author":"M. D. Berman","year":"1978","unstructured":"Berman, M. D.; Kleinman, H. K.; Waggoner, J. G., et al. Synthesis and binding of collagen by isolated hepatocytes from rats with and without hepatic fibrosis. Gastroenterology 75:955; 1978.","journal-title":"Gastroenterology"},{"key":"BF02623668_CR2","first-page":"2510","volume":"38","author":"F. Chytil","year":"1979","unstructured":"Chytil, F.; Ong, D. E. Cellular retinol- and retinoic acid-binding proteins in vitamin A action. Fed. Proc. 38:2510\u20132514; 1979.","journal-title":"Fed. Proc."},{"key":"BF02623668_CR3","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1002\/jcp.1041300104","volume":"130","author":"J. L. Dixon","year":"1987","unstructured":"Dixon, J. L.; Goodman, D. S. Studieson the metabolism of retinol-binding protein by primary hepatocytes from retinol-deficient rats. J. Cell. Physiol. 130:14\u201320; 1987.","journal-title":"J. Cell. Physiol."},{"key":"BF02623668_CR4","first-page":"2501","volume":"38","author":"D. S. Goodman","year":"1979","unstructured":"Goodman, D. S. Vitamin A and retinoids: recent advance. Introduction, background, and general overview. Fed. Proc. 38:2501\u20132503; 1979.","journal-title":"Fed. Proc."},{"key":"BF02623668_CR5","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/0076-6879(71)17252-7","volume":"17A","author":"D. K. Granner","year":"1970","unstructured":"Granner, D. K.; Tomkins, G. M. Tyrosine aminotransferase (rat liver). Methods Enzymol. 17A:633\u2013637; 1970.","journal-title":"Methods Enzymol."},{"key":"BF02623668_CR6","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0014-4827(83)90107-6","volume":"143","author":"C. Guguen-Guillouzo","year":"1983","unstructured":"Guguen-Guillouzo, C.; Clement, B.; Baffet, G., et al. Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another epithelial cell type. Exp. Cell Res. 143:47\u201354; 1983.","journal-title":"Exp. Cell Res."},{"key":"BF02623668_CR7","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/0006-291X(84)91255-5","volume":"120","author":"A. Guillouzo","year":"1984","unstructured":"Guillouzo, A.; Delers, F.; Clement, B., et al. Long-term production of acute-phase proteins by adult rat hepatocytes cocultured with another liver cell type in serum-free medium. Biochem. Biophys. Res. Commun. 120:311\u2013317; 1984.","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"BF02623668_CR8","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/S0076-6879(79)58126-9","volume":"58","author":"R. G. Ham","year":"1979","unstructured":"Ham, R. G.; McKeehan, W. L. Media and growth requirements. Methods Enzymol. 58:44\u201393; 1979.","journal-title":"Methods Enzymol."},{"key":"BF02623668_CR9","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1093\/oxfordjournals.jbchem.a134130","volume":"92","author":"Y. Horiuti","year":"1982","unstructured":"Horiuti, Y.; Nakamura, T.; Ichihara, A. Role of serum in maintenance of functional hepatocytes in primary culture. J. Biochem. 92:1985\u20131994; 1982.","journal-title":"J. Biochem."},{"key":"BF02623668_CR10","doi-asserted-by":"crossref","first-page":"3252","DOI":"10.1073\/pnas.82.10.3252","volume":"82","author":"H. C. Isom","year":"1985","unstructured":"Isom, H. C.; Secott, T.; Georgoff, I., et al. Maintenance of differentiated rat hepatocytes in primary culture. Proc. Natl. Acad. Sci. USA 82:3252\u20133256; 1985.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BF02623668_CR11","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1172\/JCI105889","volume":"47","author":"M. Kanai","year":"1968","unstructured":"Kanai, M.; Raz, A.; Goodman, D. S. Retinol-binding protein: the transport protein for vitamin A in human plasma. J. Clin. Invest. 47:2025\u20132044; 1968.","journal-title":"J. Clin. Invest."},{"key":"BF02623668_CR12","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0091-679X(08)60477-X","volume":"14","author":"H. Katsuta","year":"1976","unstructured":"Katsuta, H.; Takaoka, T. Improved synthetic media suitable for tissue culture of various mammalian cells. Methods Cell Biol. 14:145\u2013158; 1976.","journal-title":"Methods Cell Biol."},{"key":"BF02623668_CR13","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/BF02796495","volume":"12","author":"B. A. Laishes","year":"1976","unstructured":"Laishes, B. A.; Williams, G. M. Conditions affecting primary cell cultures of functional adult rat hepatocytes. I. The effect of insulin. In Vitro 12:521\u2013532; 1976.","journal-title":"In Vitro"},{"key":"BF02623668_CR14","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1007\/BF02796367","volume":"12","author":"B. A. Laishes","year":"1976","unstructured":"Laishes, B. A.; Williams, G. M., Conditions affecting primary cell cultures of functional adult rat hepatocytes. II. Dexamethasone enhanced longevity and maintenance of morphology. In Vitro 12:821\u2013832; 1976.","journal-title":"In Vitro"},{"key":"BF02623668_CR15","first-page":"3509","volume":"39","author":"R. Langenbach","year":"1979","unstructured":"Langenbach, R.; Malick, L.; Tompa, A., et al. Maintenance of adult rat hepatocytes on C3H\/10T1\/2 cells. Cancer Res. 39:3509\u20133514; 1979.","journal-title":"Cancer Res."},{"key":"BF02623668_CR16","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","author":"O. H. Lowry","year":"1951","unstructured":"Lowry, O. H.; Rosebrough, N. J.; Farr, A. L., et al. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265\u2013275; 1951.","journal-title":"J. Biol. Chem."},{"key":"BF02623668_CR17","first-page":"57","volume":"51","author":"A. Martinez-Hernandez","year":"1984","unstructured":"Martinez-Hernandez, A. The hepatic extracellular matrix. I. Electron immunohistochemical studies in normal rat liver. Lab. Invest. 51:57\u201374; 1984.","journal-title":"Lab. Invest."},{"key":"BF02623668_CR18","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/0014-4827(75)90532-7","volume":"94","author":"G. Michalopoulos","year":"1975","unstructured":"Michalopoulos, G.; Pitot, H. C. Primary culture of parenchymal liver cells on collagen membranes. Morphological and biochemical observations. Exp. Cell Res. 94:70\u201378; 1975.","journal-title":"Exp. Cell Res."},{"key":"BF02623668_CR19","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1007\/BF02618306","volume":"15","author":"G. Michalopoulos","year":"1979","unstructured":"Michalopoulos, G.; Russell, F.; Biles, C. Primary cultures of hepatocytes on human fibroblasts. In Vitro 15:796\u2013806; 1979.","journal-title":"In Vitro"},{"key":"BF02623668_CR20","first-page":"351","volume":"31","author":"M. Miyazaki","year":"1977","unstructured":"Miyazaki, M. Primary culture of adult rat liver cells. I. Preparation of isolated cells from trypsin-perfused liver of adult rat. Acta Med. Okayama 31:351\u2013360; 1977.","journal-title":"Acta Med. Okayama"},{"key":"BF02623668_CR21","first-page":"11","volume":"32","author":"M. Miyazaki","year":"1978","unstructured":"Miyazaki, M. Primary culture of adult rat liver cells. II. Cytological and biochemical properties of primary cultured cells. Acta Med. Okayama 32:11\u201322; 1978.","journal-title":"Acta Med. Okayama"},{"key":"BF02623668_CR22","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/S0014-4827(85)80047-1","volume":"159","author":"M. Miyazaki","year":"1985","unstructured":"Miyazaki, M.; Handa, Y.; Oda, M., et al. Long-term survival of functional hepatocytes from adult rat in the presence of phenobarbital in primary culture. Exp. Cell Res. 159:176\u2013190; 1985.","journal-title":"Exp. Cell Res."},{"key":"BF02623668_CR23","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/BF01851972","volume":"187","author":"M. Miyazaki","year":"1987","unstructured":"Miyazaki, M.; Handa, Y.; Suzuki, Y., et al. Effect of various barbituric acid derivatives on survival of functional hepatocytes from adult rats in primary culture. Res. Exp. Med. 187:105\u2013117; 1987.","journal-title":"Res. Exp. Med."},{"key":"BF02623668_CR24","first-page":"2","volume":"23","author":"M. Miyazaki","year":"1987","unstructured":"Miyazaki, M.; Handa, Y.; Suzuki, Y., et al. Synthesis of barbituric acid derivatives and their effect on survival of functional hepatocytes from adult rats in primary culture. In Vitro 23:2\u20139; 1987.","journal-title":"In Vitro"},{"key":"BF02623668_CR25","first-page":"351","volume":"42","author":"M. Miyazaki","year":"1988","unstructured":"Miyazaki, M.; Suzuki, Y.; Sato, J. A method for rapid preparation of single-cell suspensions from rat hepatocyte cultures on collagen substratum and the mechanism of cell dissociation. Acta Med. Okayama 42:351\u2013354; 1988.","journal-title":"Acta Med. Okayama"},{"key":"BF02623668_CR26","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/BF01852393","volume":"184","author":"M. Miyazaki","year":"1984","unstructured":"Miyazaki, M.; Tsunashima, M.; Wahid, S., et al. Comparison of cytologic and biochemical properties between liver cells isolated from adult rats by trypsin perfusion and those isolated by collagenase perfusion. Res. Exp. Med. 184:191\u2013204; 1984.","journal-title":"Res. Exp. Med."},{"key":"BF02623668_CR27","first-page":"85","volume":"32","author":"M. Miyazaki","year":"1978","unstructured":"Miyazaki, M.; Watanabe, A.; Shiraishi, M., et al. Primary culture of adult rat liver cells. III. Hormonal effects on cytological and biochemical properties of primary cultured cells. Acta Med. Okayama 32:85\u201396; 1978.","journal-title":"Acta Med. Okayama"},{"key":"BF02623668_CR28","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/0014-4827(84)90769-9","volume":"155","author":"T. Nakamura","year":"1984","unstructured":"Nakamura, T.; Asami, O.; Tanaka, K., et al. Increased survival of rat hepatocytes in serum-free medium by inhibition of a trypsin-like protease associated with their plasma membranes. Exp. Cell Res. 155:81\u201391; 1984.","journal-title":"Exp. Cell Res."},{"key":"BF02623668_CR29","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1083\/jcb.87.1.255","volume":"87","author":"M. Rojkind","year":"1980","unstructured":"Rojkind, M.; Gatmaitan, Z.; Mackensen, S., et al. Connective tissue biomatrix: its isolation and utilization for long-term cultures of normal rat hepatocytes. J. Cell Biol. 87:255\u2013263; 1980.","journal-title":"J. Cell Biol."},{"key":"BF02623668_CR30","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/0014-4827(78)90488-3","volume":"114","author":"C. R. Savage Jr.","year":"1978","unstructured":"Savage, C. R., Jr.; Bonney, R. J. Extended expression of differentiated function in primary cultures of adult liver parenchymal cells maintained on nitrocellulose filters. I. Induction of phosphoenolpyruvate carboxykinase and tyrosine aminotransferase. Exp. Cell Res. 114:307\u2013315; 1978.","journal-title":"Exp. Cell Res."},{"key":"BF02623668_CR31","first-page":"267","volume":"23","author":"N. Sawada","year":"1987","unstructured":"Sawada, N.; Tomomura, A.; Sattler, C. A., et al. Effect of extracellular matrix components on the growth and differentiation of cultured rat hepatocytes. In Vitro 23:267\u2013273; 1987.","journal-title":"In Vitro"},{"key":"BF02623668_CR32","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0091-679X(08)61797-5","volume":"13","author":"P. O. Seglen","year":"1976","unstructured":"Seglen, P. O. Preparation of isolated rat liver cells. Methods Cell Biol. 13:29\u201383; 1976.","journal-title":"Methods Cell Biol."},{"key":"BF02623668_CR33","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1210\/endo-111-3-919","volume":"111","author":"M. H. Simonian","year":"1982","unstructured":"Simonian, M. H.; White, M. L.; Gill, G. N. Growth and function of cultured bovine adrenocortical cells in a serum-free defined medium. Endocrinology 111:919\u2013927; 1982.","journal-title":"Endocrinology"},{"key":"BF02623668_CR34","first-page":"3259","volume":"40","author":"A. E. Sirica","year":"1980","unstructured":"Sirica, A. E.; Hwang, C. G.; Sattler, G. L. et al. Use of primary cultures of adult rat hepatocytes on collagen gel-nylon mesh to evaluate carcinogen-induced unscheduled DNA synthesis. Cancer Res. 40:3259\u20133267; 1980.","journal-title":"Cancer Res."},{"key":"BF02623668_CR35","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1093\/oxfordjournals.jbchem.a134479","volume":"94","author":"K. Tanaka","year":"1983","unstructured":"Tanaka, K.; Ichihara, A. Different effects of amino acid deprivation on synthesis of intra- and extracellular proteins in rat hepatocytes in primary culture. J. Biochem. 94:1339\u20131348; 1983.","journal-title":"J. Biochem."},{"key":"BF02623668_CR36","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1093\/oxfordjournals.jbchem.a132618","volume":"86","author":"K. Tanaka","year":"1979","unstructured":"Tanaka, K.; Kishi, K.; Ichihara, A. Biochemical studies on liver functions in primary cultured hepatocytes of adult rats. II. Regulation of protein and amino acid metabolism. J. Biochem. 86:863\u2013870; 1979.","journal-title":"J. Biochem."},{"key":"BF02623668_CR37","doi-asserted-by":"crossref","first-page":"2610","DOI":"10.1016\/S0021-9258(17)35831-3","volume":"261","author":"K. Tanaka","year":"1986","unstructured":"Tanaka, K.; Nakamura, T.; Ichihara, A. A unique trypsin-like protease associated with plasma membranes of rat liver. J. Biol. Chem. 261:2610\u20132615; 1986.","journal-title":"J. Biol. Chem."},{"key":"BF02623668_CR38","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1093\/jnci\/22.5.1003","volume":"22","author":"C. Waymouth","year":"1959","unstructured":"Waymouth, C. Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752\/1). JNCI 22:1003\u20131017; 1959.","journal-title":"JNCI"},{"key":"BF02623668_CR39","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1007\/BF02615128","volume":"13","author":"G. M. Williams","year":"1977","unstructured":"Williams, G. M.; Bermudez, E.; Scarmuzzino, D. Rat hepatocyte primary cell cultures. III. Improved dissociation and attachment techniques and the enhancement of survival by culture medium. In Vitro 13:809\u2013817; 1977.","journal-title":"In Vitro"},{"key":"BF02623668_CR40","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/0014-4827(71)90316-8","volume":"69","author":"G. M. Williams","year":"1971","unstructured":"Williams, G. M.; Weisburger, E. K.; Weisburger, J. H. Isolation and long-term cell culture of epithelial-like cells from rat liver. Exp. Cell Res. 69:106\u2013112; 1971.","journal-title":"Exp. Cell Res."},{"key":"BF02623668_CR41","doi-asserted-by":"crossref","first-page":"385","DOI":"10.2183\/pjab.57.385","volume":"57","author":"I. Yamane","year":"1981","unstructured":"Yamane, I.; Kan, M.; Minamoto, Y., et al. Alpha-cyclodextrin, a novel substitute for bovine albumin in serum-free culture of mammalian cells. Jpn. Proc. Natl. Acad. Sci. 57:385\u2013389; 1981.","journal-title":"Jpn. Proc. Natl. Acad. Sci."}],"container-title":["In Vitro Cellular & Developmental Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/BF02623668.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/BF02623668\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/BF02623668","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T03:14:49Z","timestamp":1628824489000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/BF02623668"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,9]]},"references-count":41,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1989,9]]}},"alternative-id":["BF02623668"],"URL":"https:\/\/doi.org\/10.1007\/bf02623668","relation":{},"ISSN":["0883-8364","1475-2689"],"issn-type":[{"value":"0883-8364","type":"print"},{"value":"1475-2689","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,9]]}}}