{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:39:34Z","timestamp":1788925174712,"version":"build-2803163510"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,5,24]],"date-time":"2025-05-24T00:00:00Z","timestamp":1748044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,5,24]],"date-time":"2025-05-24T00:00:00Z","timestamp":1748044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5R35GM149345-02"],"award-info":[{"award-number":["5R35GM149345-02"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Inflamm. Res."],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the micro-RNA (miRNA) cargo of alveolar small extracellular vesicles (sEVs) after lung contusion (LC), which can contribute to the development of trauma-related acute lung injury (ALI).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A mouse model of LC was conducted with a controlled cortical impact device. Bronchoalveolar lavage fluid (BAL) was collected 24\u00a0h post-injury and sEVs were purified using size exclusion chromatography. sEVs characteristics and miRNA cargo were analyzed with vesicle flow cytometry and sequencing. Macrophages were treated with BAL sEVs in vitro to assess their pro-inflammatory effect.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>LC increased lung permeability and caused ALI histologically. LC increased the number of sEVs in the BAL and altered their miRNA cargo. BAL sEVs collected after LC increased pro-inflammatory cytokine release from macrophages.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>LC increased the mobilization of sEVs to the alveolar space and modified their miRNA cargo that might contribute to the development of ALI by activating the immune response in macrophages.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00011-025-02051-2","type":"journal-article","created":{"date-parts":[[2025,5,24]],"date-time":"2025-05-24T14:49:45Z","timestamp":1748098185000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Lung contusion drives lung injury by modifying miRNA cargo in alveolar small extracellular vesicles"],"prefix":"10.1007","volume":"74","author":[{"given":"Keita","family":"Nakatsutsumi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Jun","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wooil","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Johnston","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"Pool","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Kezios","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raul","family":"Coimbra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian P.","family":"Eliceiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd W.","family":"Costantini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,5,24]]},"reference":[{"key":"2051_CR1","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1097\/SHK.0000000000001109","volume":"50","author":"IE Brown","year":"2018","unstructured":"Brown IE, Rigor RR, Schutzman LM, Khosravi N, Chung K, Becker JA, et al. Pulmonary arterial thrombosis in a murine model of blunt thoracic trauma. Shock. 2018;50:696\u2013705.","journal-title":"Shock"},{"key":"2051_CR2","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1097\/SHK.0b013e31819c385c","volume":"32","author":"K Raghavendran","year":"2009","unstructured":"Raghavendran K, Notter RH, Davidson BA, Helinski JD, Kunkel SL, Knight PR. Lung contusion: inflammatory mechanisms and interaction with other injuries. Shock. 2009;32:122\u201330.","journal-title":"Shock"},{"key":"2051_CR3","doi-asserted-by":"crossref","unstructured":"Piszczatowska K, Czerwaty K, Cyran AM, Fiedler M, Ludwig N, Brzost J et al. The emerging role of small extracellular vesicles in inflammatory airway diseases. Diagnostics (Basel). 2021;11.","DOI":"10.3390\/diagnostics11020222"},{"key":"2051_CR4","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1002\/jcp.30553","volume":"237","author":"Z Liu","year":"2022","unstructured":"Liu Z, Yan J, Tong L, Liu S, Zhang Y. The role of exosomes from BALF in lung disease. J Cell Physiol. 2022;237:161\u20138.","journal-title":"J Cell Physiol"},{"key":"2051_CR5","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1097\/shk.0b013e318048801a","volume":"28","author":"JJ Hoth","year":"2007","unstructured":"Hoth JJ, Hudson WP, Brownlee NA, Yoza BK, Hiltbold EM, Meredith JW, et al. Toll-like receptor 2 participates in the response to lung injury in a murine model of pulmonary contusion. Shock. 2007;28:447\u201352.","journal-title":"Shock"},{"key":"2051_CR6","doi-asserted-by":"crossref","unstructured":"Park DJ, Choi W, Sayeed S, Dorschner RA, Rainaldi J, Ho K et al. Defining the activity of pro-reparative extracellular vesicles in wound healing based on MiRNA payloads and cell type-specific lineage mapping. Mol Ther. 2024.","DOI":"10.1016\/j.ymthe.2024.02.019"},{"key":"2051_CR7","doi-asserted-by":"crossref","unstructured":"Guiot J, Struman I, Louis E, Louis R, Malaise M, Njock MS. Exosomal MiRNAs in lung diseases: from biologic function to therapeutic targets. J Clin Med. 2019;8.","DOI":"10.3390\/jcm8091345"},{"key":"2051_CR8","doi-asserted-by":"crossref","unstructured":"Kim TH, Hong SB, Lim CM, Koh Y, Jang EY, Huh JW. The role of exosomes in bronchoalveloar lavage from patients with acute respiratory distress syndrome. J Clin Med. 2019;8.","DOI":"10.3390\/jcm8081148"},{"key":"2051_CR9","doi-asserted-by":"publisher","first-page":"646546","DOI":"10.3389\/fcimb.2021.646546","volume":"11","author":"F Liu","year":"2021","unstructured":"Liu F, Peng W, Chen J, Xu Z, Jiang R, Shao Q, et al. Exosomes derived from alveolar epithelial cells promote alveolar macrophage activation mediated by miR-92a-3p in Sepsis-Induced acute lung injury. Front Cell Infect Microbiol. 2021;11:646546.","journal-title":"Front Cell Infect Microbiol"},{"key":"2051_CR10","doi-asserted-by":"publisher","first-page":"1500","DOI":"10.4049\/jimmunol.1800264","volume":"201","author":"H Lee","year":"2018","unstructured":"Lee H, Zhang D, Laskin DL, Jin Y. Functional evidence of pulmonary extracellular vesicles in infectious and noninfectious lung inflammation. J Immunol. 2018;201:1500\u20139.","journal-title":"J Immunol"},{"key":"2051_CR11","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.imlet.2020.12.015","volume":"231","author":"R Xu","year":"2021","unstructured":"Xu R, Shao Z, Cao Q. MicroRNA-144-3p enhances LPS induced septic acute lung injury in mice through downregulating Caveolin-2. Immunol Lett. 2021;231:18\u201325.","journal-title":"Immunol Lett"},{"key":"2051_CR12","first-page":"201","volume":"68","author":"Hangqi Ren1 WMaQX","year":"2021","unstructured":"Hangqi Ren1 WMaQX. miR-19a-3p Inhibition alleviates sepsis-induced lung injury via enhancing USP13 expression. Acta Biochim Pol. 2021;68:201.","journal-title":"Acta Biochim Pol"},{"key":"2051_CR13","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.3389\/fimmu.2017.01327","volume":"8","author":"F Huang","year":"2017","unstructured":"Huang F, Zhao JL, Wang L, Gao CC, Liang SQ, An DJ, et al. miR-148a-3p mediates Notch signaling to promote the differentiation and M1 activation of macrophages. Front Immunol. 2017;8:1327.","journal-title":"Front Immunol"},{"key":"2051_CR14","doi-asserted-by":"publisher","first-page":"7435","DOI":"10.4049\/jimmunol.1000798","volume":"185","author":"QY Zhu","year":"2010","unstructured":"Zhu QY, Liu Q, Chen JX, Lan K, Ge BX. MicroRNA-101 targets MAPK phosphatase-1 to regulate the activation of MAPKs in macrophages. J Immunol. 2010;185:7435\u201342.","journal-title":"J Immunol"},{"key":"2051_CR15","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1007\/s00011-021-01437-2","volume":"70","author":"H Zhong","year":"2021","unstructured":"Zhong H, Lin H, Pang Q, Zhuang J, Liu X, Li X, et al. Macrophage ICAM-1 functions as a regulator of phagocytosis in LPS induced endotoxemia. Inflamm Res. 2021;70:193\u2013203.","journal-title":"Inflamm Res"},{"key":"2051_CR16","doi-asserted-by":"publisher","first-page":"L962","DOI":"10.1152\/ajplung.00352.2005","volume":"290","author":"MP Mendez","year":"2006","unstructured":"Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R. 3rd. Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2006;290:L962\u201370.","journal-title":"Am J Physiol Lung Cell Mol Physiol"}],"container-title":["Inflammation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00011-025-02051-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00011-025-02051-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00011-025-02051-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T16:55:27Z","timestamp":1767200127000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00011-025-02051-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,24]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["2051"],"URL":"https:\/\/doi.org\/10.1007\/s00011-025-02051-2","relation":{},"ISSN":["1023-3830","1420-908X"],"issn-type":[{"value":"1023-3830","type":"print"},{"value":"1420-908X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,24]]},"assertion":[{"value":"21 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 May 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 May 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 May 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"83"}}