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Mol. Life Sci."],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Loss of GLI-Similar 3 (GLIS3) function in mice and humans causes congenital hypothyroidism (CH). In this study, we demonstrate that GLIS3 protein is first detectable at E15.5 of murine thyroid development, a time at which GLIS3 target genes, such as\n                    <jats:italic>Slc5a5<\/jats:italic>\n                    (\n                    <jats:italic>Nis<\/jats:italic>\n                    ), become expressed. This, together with observations showing that ubiquitous\n                    <jats:italic>Glis3<\/jats:italic>\n                    KO mice do not display major changes in prenatal thyroid gland morphology, indicated that CH in\n                    <jats:italic>Glis3<\/jats:italic>\n                    KO mice is due to dyshormonogenesis rather than thyroid dysgenesis. Analysis of GLIS3 in postnatal thyroid suggested a link between GLIS3 protein expression and blood TSH levels. This was supported by data showing that treatment with TSH, cAMP, or adenylyl cyclase activators or expression of constitutively active PKA enhanced GLIS3 protein stability and transcriptional activity, indicating that GLIS3 activity is regulated at least in part by TSH\/TSHR-mediated activation of PKA. The TSH-dependent increase in GLIS3 transcriptional activity would be critical for the induction of GLIS3 target gene expression, including several thyroid hormone (TH) biosynthetic genes, in thyroid follicular cells of mice fed a low iodine diet (LID) when blood TSH levels are highly elevated. Like TH biosynthetic genes, the expression of cell cycle genes is suppressed in ubiquitous\n                    <jats:italic>Glis3<\/jats:italic>\n                    KO mice fed a LID; however, in thyroid-specific\n                    <jats:italic>Glis3<\/jats:italic>\n                    knockout mice, the expression of cell cycle genes was not repressed, in contrast to TH biosynthetic genes. This indicated that the inhibition of cell cycle genes in ubiquitous\n                    <jats:italic>Glis3<\/jats:italic>\n                    KO mice is dependent on changes in gene expression in GLIS3 target tissues other than the thyroid.\n                  <\/jats:p>","DOI":"10.1007\/s00018-024-05113-6","type":"journal-article","created":{"date-parts":[[2024,1,28]],"date-time":"2024-01-28T00:02:43Z","timestamp":1706400163000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["GLIS3 expression in the thyroid gland in relation to TSH signaling and regulation of gene expression"],"prefix":"10.1007","volume":"81","author":[{"given":"Hong Soon","family":"Kang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara A.","family":"Grimm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Hui","family":"Liao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0954-4445","authenticated-orcid":false,"given":"Anton M.","family":"Jetten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,1,28]]},"reference":[{"key":"5113_CR1","doi-asserted-by":"publisher","first-page":"110638","DOI":"10.1016\/j.mce.2019.110638","volume":"501","author":"HM Targovnik","year":"2020","unstructured":"Targovnik HM, Scheps KG, Rivolta CM (2020) Defects in protein folding in congenital hypothyroidism. 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