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It is hypothesized that abietanes exert their antibacterial effects by disrupting microbial cell walls. Recent studies have classified\n                    <jats:italic>Plectranthus s.l<\/jats:italic>\n                    . abietanes into six distinct groups. However, the impact of this chemical diversity on the response of abietanes against Gram-positive bacteria remains unexamined. Therefore, a structure-activity relationship study on abietanes from species of\n                    <jats:italic>Plectranthus sensu stricto<\/jats:italic>\n                    and\n                    <jats:italic>Coleus<\/jats:italic>\n                    was conducted to identify active fragments or pharmacophores in abietane-type diterpenoids that could be responsible for their activity against the Gram-positive bacteria\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    . The study followed a bio-guided approach and included chemical profiling of the extracts, performed using NMR spectroscopy, and comparative analysis, using LC-MS in both positive and negative ionization modes. Results revealed all six classes of abietanes occurred as major constituents of the extracts, with distinctive chemical markers differentiating between\n                    <jats:italic>Plectranthus s.s<\/jats:italic>\n                    . and\n                    <jats:italic>Coleus<\/jats:italic>\n                    species. Oxidation of the B-ring in acylhydrobenzoquinones was identified as a key factor contributing to the strong antibacterial activity of\n                    <jats:italic>Plectranthus s.l<\/jats:italic>\n                    . derived compounds against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    . Analysis of 28 species of\n                    <jats:italic>Plectranthus s.l<\/jats:italic>\n                    . revealed four main clusters based on the accumulation of various abietane classes. The active acylhydrobenzoquinones were a defining factor in the separation of one of the groups of plants while another cluster in both\n                    <jats:italic>Plectranthus s.s<\/jats:italic>\n                    . and\n                    <jats:italic>Coleus<\/jats:italic>\n                    separated based on flavonoid contents. It would be of interest to examine how this trend might be broadened to encompass all species within each genus.\n                  <\/jats:p>","DOI":"10.1007\/s00044-026-03524-7","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T11:24:13Z","timestamp":1768303453000},"page":"548-562","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Acylhydrobenzoquinones influence the susceptibility of Staphylococcus aureus towards abietanes and the speciation within Plectranthus sensus lato"],"prefix":"10.1007","volume":"35","author":[{"given":"Gabin Thierry M.","family":"Bitchagno","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sohini S.","family":"Bhatia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott","family":"Bintrim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Coates","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debborah","family":"Mulligan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique S. 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