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During orthodontic treatment, sterile inflammation and mechanical loading favor the production of receptor activator of NF-\u03baB ligand (RANKL). Simultaneously, expression of osteoprotegerin (OPG) is inhibited. This stimulates bone resorption on the pressure side. Recently, heat shock protein\u00a027 (HSP27) was shown to be expressed in the periodontal ligament after force application and to interfere with inflammatory processes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>We investigated the effects of phosphorylated HSP27 on collagen synthesis (<jats:italic>COL1A2 <\/jats:italic>mRNA), inflammation (<jats:italic>IL1B<\/jats:italic> mRNA, <jats:italic>IL6<\/jats:italic> mRNA, PTGS2 protein) and bone remodeling (RANKL protein, OPG protein) in human periodontal ligament fibroblasts (PDLF) without and with transfection of a\u00a0plasmid mimicking permanent phosphorylation of HSP27 using real-time quantitative polymerase chain reaction (RT-qPCR), western blot and enzyme-linked immunosorbent assays (ELISAs). Furthermore, we investigated PDLF-induced osteoclastogenesis after compressive strain in a\u00a0co-culture model with human macrophages.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In particular, phosphorylated HSP27 increased gene expression of <jats:italic>COL1A2<\/jats:italic> and protein expression of PTGS2, while <jats:italic>IL6<\/jats:italic> mRNA levels were reduced. Furthermore, we observed an increasing effect on the RANKL\/OPG ratio and osteoclastogenesis mediated by PDLF.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Phosphorylation of HSP27 may therefore be involved in the regulation of orthodontic tooth movement by impairment of the sterile inflammation response and osteoclastogenesis.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00056-022-00391-w","type":"journal-article","created":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T15:05:11Z","timestamp":1650553511000},"page":"143-153","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Impact of phosphorylation of heat shock protein\u00a027 on the expression profile of periodontal ligament fibroblasts during mechanical strain","Einfluss der Phosphorylierung des Hitzeschockproteins\u00a027 auf das Expressionsprofil von parodontalen Ligamentfibroblasten bei mechanischer Belastung"],"prefix":"10.1007","volume":"84","author":[{"given":"Agnes","family":"Schr\u00f6der","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Cieplik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerrit","family":"Spanier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Proff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Kirschneck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,4,21]]},"reference":[{"issue":"2","key":"391_CR1","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1016\/j.yexcr.2004.05.032","volume":"299","author":"AS Adhikari","year":"2004","unstructured":"Adhikari\u00a0AS, Sridhar Rao\u00a0K, Rangaraj\u00a0N et al (2004) Heat stress-induced localization of small heat shock proteins in mouse myoblasts: intranuclear lamin A\/C speckles as target for alphaB-crystallin and Hsp25. 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Furthermore, no part of this article has been published before or is considered for publication elsewhere.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All experiments were performed in accordance with the relevant guidelines and regulations. Approval for the collection and use of PDLF was obtained from the ethics committee of the University of Regensburg, Germany (approval number 12-170-0150). We obtained informed consent from all patients or their legal guardians.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical standards"}}]}}