{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T12:42:05Z","timestamp":1740141725383,"version":"3.37.3"},"reference-count":11,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T00:00:00Z","timestamp":1671667200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T00:00:00Z","timestamp":1671667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100023448","name":"Robert Koch-Institut","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100023448","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Bundesgesundheitsbl"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title><jats:sec>\n                <jats:title>Hintergrund<\/jats:title>\n                <jats:p>In den vergangenen Jahren hat sich die Gesamtgenomsequenzierung (\u201ewhole genome sequencing\u201c; WGS) in Kombination mit bioinformatischen Analysen zum Stand der Technik bei der Bewertung des Pathogenit\u00e4ts- und Resistenzpotenzials sowie der Verwandtschaftsgrade zwischen Bakterien entwickelt. Die WGS-Analyse stellt somit ein zentrales Instrument bei der Typisierung von Erregern und der Untersuchung von Krankheits- und Ausbruchsclustern im Rahmen der molekularen Epidemiologie dar. Ziel der Studie war die Generierung eines \u00dcberblicks der in Deutschland auf Landes- und Bundesebene verf\u00fcgbaren Erregertypisiermethoden von Salmonellen und Shiga-Toxin-bildenden bzw. enteroh\u00e4morrhagischen <jats:italic>Escherichia coli<\/jats:italic> (STEC\/EHEC) und den angewandten geno- und ph\u00e4notypischen Methoden sowie \u00fcber die Verf\u00fcgbarkeit der genombasierten Typisierung und entsprechenden Analyseverfahren.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methoden<\/jats:title>\n                <jats:p>Im Zeitraum vom Februar bis Juni 2020 wurde eine elektronische Umfrage bei Laboratorien durchgef\u00fchrt, die f\u00fcr den \u00f6ffentlichen Gesundheitsschutz und gesundheitlichen Verbraucherschutz t\u00e4tig sind.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Ergebnisse und Fazit<\/jats:title>\n                <jats:p>Die Ergebnisse der Umfrage zeigten, dass viele der teilnehmenden Laboratorien \u00fcber eine gro\u00dfe Auswahl an ph\u00e4notypischen und molekularbiologischen Methoden verf\u00fcgen. Molekularbiologische Typisierungen werden am h\u00e4ufigsten f\u00fcr die Speziesidentifizierung von Salmonellen herangezogen. WGS-Verfahren sind vielfach schon bei Einrichtungen auf Bundes- und Landesebene etabliert oder befinden sich im Aufbau. Die Illumina-Sequenzierung ist dabei die am weitesten verbreitete Technologie. Die Umfrage best\u00e4tigt die Bedeutung von molekularbiologischen und genombasierten Typisierungstechnologien f\u00fcr die Laboratorien bei der Diagnostik von bakteriellen zoonotischen Erregern.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00103-022-03622-y","type":"journal-article","created":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T12:04:43Z","timestamp":1671710683000},"page":"75-83","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bestandsaufnahme der verf\u00fcgbaren und aktuell eingesetzten Typisierungsmethoden einschlie\u00dflich genombasierter Verfahren von Zoonoseerregern am Beispiel von Salmonella enterica","Assessment of available and currently applied typing methods including genome-based methods for zoonotic pathogens with a focus on Salmonella enterica"],"prefix":"10.1007","volume":"66","author":[{"given":"Michael","family":"Pietsch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Simon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Richter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Burkhard","family":"Malorny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Uelze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina","family":"Hepner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Dangel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Sing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid","family":"Huber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Busch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rg","family":"Linde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Methner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalie","family":"Becker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido","family":"Werner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Mellmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelika","family":"Fruth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antje","family":"Flieger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,12,22]]},"reference":[{"key":"3622_CR1","doi-asserted-by":"publisher","DOI":"10.2900\/1244210.2900\/12442","volume-title":"Expert opinion on whole genome sequencing for public health surveillance","author":"European Centre for Disease Prevention and Control","year":"2016","unstructured":"European Centre for Disease Prevention and Control (2016) Expert opinion on whole genome sequencing for public health surveillance. 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F\u00fcr die aufgef\u00fchrten Studien gelten die jeweils dort angegebenen ethischen Richtlinien.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Interessenkonflikt"}}]}}