{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T11:37:45Z","timestamp":1782473865811,"version":"3.54.5"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T00:00:00Z","timestamp":1619740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T00:00:00Z","timestamp":1619740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Urologe"],"published-print":{"date-parts":[[2021,5]]},"DOI":"10.1007\/s00120-021-01537-1","type":"journal-article","created":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T10:03:00Z","timestamp":1619776980000},"page":"647-656","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["\u201ePraktisches Spermiogramm\u201c\u00a0\u2013 Ejakulatdiagnostik gem\u00e4\u00df WHO","\u201cPractical spermiogram\u201d\u2014semen analysis according to WHO recommendations"],"prefix":"10.1007","volume":"60","author":[{"given":"Sabine","family":"Kliesch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,30]]},"reference":[{"key":"1537_CR1","volume-title":"WHO Laboratory manual for the examination and processing of human sperm","author":"World Health Organization","year":"2010","unstructured":"World Health Organization (2010) WHO Laboratory manual for the examination and processing of human sperm, 5.\u00a0Aufl. WHO, Geneva (herausgegeben und \u00fcbersetzt von Nieschlag E, Schlatt S, Behre HM et\u00a0al. (2012) WHO-Laborhandbuch zur Untersuchung und Aufarbeitung des menschlichen Ejakulates. Springer-Verlag, Heidelberg)","edition":"5"},{"key":"1537_CR2","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12983","author":"MJ Campbell","year":"2021","unstructured":"Campbell MJ, Lotti F, Baldi E et\u00a0al (2021) Distribution of Semen Examination Results 2020\u2014a\u00a0follow up of data collated for the WHO semen analysis manual 2010. Andrology. https:\/\/doi.org\/10.1111\/andr.12983","journal-title":"Andrology"},{"key":"1537_CR3","first-page":"A1","volume":"116","author":"Bundes\u00e4rztekammer","year":"2019","unstructured":"Bundes\u00e4rztekammer (2019) Richtlinie der Bundes\u00e4rztekammer zur Qualit\u00e4tssicherung laboratoriumsmedizinischer Untersuchungen gem\u00e4\u00df des Beschlusses des Vorstands der Bundes\u00e4rztekammer in seiner Sitzung am 18.10.2019. Dtsch Arztebl 116:A1\u2013A33","journal-title":"Dtsch Arztebl"},{"key":"1537_CR4","unstructured":"Bundesministerium f\u00fcr Gesundheit (2017) Richtlinien des Bundesausschusses der \u00c4rzte und Krankenkassen \u00fcber \u00e4rztliche Ma\u00dfnahmen zur k\u00fcnstlichen Befruchtung (\u201eRichtlinien \u00fcber k\u00fcnstliche Befruchtung\u201c). in der Fassung vom 14. August 1990, ver\u00f6ffentlicht im Bundesarbeitsblatt 1990, Nr.\u00a012, zuletzt ge\u00e4ndert am 16. M\u00e4rz 2017, ver\u00f6ffentlicht im Bundesanzeiger BAnz AT 1. Juni 2017 B4, in Kraft getreten am 2. Juni 2017"},{"key":"1537_CR5","first-page":"A1","volume":"115","author":"Bundes\u00e4rztekammer","year":"2018","unstructured":"Bundes\u00e4rztekammer (2018) Richtlinie zur Entnahme und \u00dcbertragung von menschlichen Keimzellen im Rahmen der assistierten Reproduktion. Dtsch Arztebl 115:A1\u2013A22","journal-title":"Dtsch Arztebl"},{"issue":"Suppl","key":"1537_CR6","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.eursup.2014.08.002","volume":"13","author":"S Kliesch","year":"2014","unstructured":"Kliesch\u00a0S (2014) Diagnosis of male infertility: diagnostic work-up of the infertile man. Eur Urol 13(Suppl):73\u201382","journal-title":"Eur Urol"},{"key":"1537_CR7","doi-asserted-by":"publisher","first-page":"1116","DOI":"10.1007\/s00120-017-0455-8","volume":"56","author":"S Kliesch","year":"2017","unstructured":"Kliesch\u00a0S (2017) Rationelle Diagnostik und Therapie des kinderlosen Mannes. Urologe 56:1116\u20131128","journal-title":"Urologe"},{"key":"1537_CR8","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1093\/humupd\/dmp048","volume":"16","author":"TG Cooper","year":"2010","unstructured":"Cooper\u00a0TG, Noonan\u00a0E, von Eckardstein\u00a0S et\u00a0al (2010) World Health Organization reference values for human semen characteristics. Hum Reprod Update 16:231\u2013245","journal-title":"Hum Reprod Update"},{"key":"1537_CR9","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1007\/s00120-010-2466-6","volume":"50","author":"F Gottardo","year":"2011","unstructured":"Gottardo\u00a0F, Kliesch\u00a0S (2011) Die Ejakulatdiagnostik \u2013 Spermiogramm nach WHO-Kriterien 2010. Urologe 50:101\u2013108","journal-title":"Urologe"},{"key":"1537_CR10","doi-asserted-by":"publisher","first-page":"1278","DOI":"10.1055\/a-1017-3389","volume":"79","author":"B Toth","year":"2019","unstructured":"Toth\u00a0B, Baston-B\u00fcst\u00a0DM, Behre\u00a0HM et al (2019) Diagnosis and therapy before assisted reproductive treatments. Guideline of the DGGG, OEGGG and SGGG (S2k level, AWMF register number 015-085, february 2019)\u2014part 1, basic assessment of the woman. Geburtsh Frauenheilk 79:1278\u20131292","journal-title":"Geburtsh Frauenheilk"},{"key":"1537_CR11","doi-asserted-by":"publisher","first-page":"1293","DOI":"10.1055\/a-1017-3478","volume":"79","author":"B Toth","year":"2019","unstructured":"Toth\u00a0B, Baston-B\u00fcst\u00a0DM, Behre\u00a0HM et al (2019) Diagnosis and Treatment before Assisted Reproductive Treatments. Guideline of the DGGG, OEGGG and SGGG (S2k Level, AWMF Register Number 015-085, February 2019)\u2014Part 2, Hemostaseology, Andrology, Genetics and History of Malignant Disease. Geburtsh Frauenheilk 79:1293\u20131308","journal-title":"Geburtsh Frauenheilk"},{"key":"1537_CR12","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1007\/s11154-015-9320-5","volume":"16","author":"E Nieschlag","year":"2015","unstructured":"Nieschlag\u00a0E, Vorona\u00a0E (2015) Doping with anabolic androgenic steroids (AAS): adverse effects on non-reproductive organs and functions. Rev Endocr Metab Disord 16:199\u2013211","journal-title":"Rev Endocr Metab Disord"},{"key":"1537_CR13","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s12610-016-0029-4","volume":"26","author":"R El Osta","year":"2016","unstructured":"El Osta\u00a0R, Almont\u00a0T, Diligent\u00a0C et\u00a0al (2016) Anabolic steroids abuse and male infertility. Basic Clin Androl 26:2","journal-title":"Basic Clin Androl"},{"key":"1537_CR14","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1016\/S1472-6483(10)61888-3","volume":"7","author":"L Bj\u00f6rndahl","year":"2003","unstructured":"Bj\u00f6rndahl\u00a0L, Kvist\u00a0U (2003) Sequence of ejaculation affects the spermatozoon as a\u00a0carrier and its message. Reprod Biomed Online 7:440\u2013448","journal-title":"Reprod Biomed Online"},{"key":"1537_CR15","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1007\/s10304-019-00274-1","volume":"17","author":"V Nordhoff","year":"2019","unstructured":"Nordhoff\u00a0V, Kliesch\u00a0S (2019) Das eine unter vielen \u2013 Spermienqualit\u00e4t und M\u00f6glichkeiten der Selektion. Gynakol Endokrinol 17:250\u2013256","journal-title":"Gynakol Endokrinol"},{"key":"1537_CR16","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1007\/s00345-013-1082-7","volume":"31","author":"W Weidner","year":"2013","unstructured":"Weidner\u00a0W, Pilatz\u00a0A, Diemer\u00a0T et\u00a0al (2013) Male urogenital infections: impact of infection and inflammation on ejaculate parameters. World J Urol 31:717\u2013723","journal-title":"World J Urol"},{"key":"1537_CR17","first-page":"339","volume":"114","author":"H-C Schuppe","year":"2017","unstructured":"Schuppe\u00a0H\u2011C, Pilatz\u00a0A, Hossain\u00a0H et al (2017) Urogenitale Infektionen als Risiko f\u00fcr m\u00e4nnliche Infertilit\u00e4t. Dtsch Arztebl Int 114:339\u2013346","journal-title":"Dtsch Arztebl Int"},{"key":"1537_CR18","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1111\/j.2047-2927.2012.00020.x","volume":"1","author":"V Nordhoff","year":"2013","unstructured":"Nordhoff\u00a0V, Sch\u00fcring\u00a0AN, Krallmann\u00a0C et al (2013) Optimizing TESE-ICSI by laser-assisted selection of immotile spermatozoa and polarization microscopy for selection of oocytes. Andrology 1:67\u201374","journal-title":"Andrology"},{"key":"1537_CR19","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1111\/andr.286","volume":"3","author":"V Nordhoff","year":"2015","unstructured":"Nordhoff\u00a0V (2015) How to select immotile but viable spermatozoa on the day of intracytoplasmic sperm injection? An embryologist\u2019s view. Andrology 3:156\u2013162","journal-title":"Andrology"},{"key":"1537_CR20","doi-asserted-by":"publisher","first-page":"1124","DOI":"10.1016\/j.fertnstert.2012.07.1059","volume":"98","author":"V S\u00e1nchez","year":"2012","unstructured":"S\u00e1nchez\u00a0V, Redmann\u00a0K, Wistuba\u00a0J et\u00a0al (2012) Oxidative DNA damage in human sperm can be detected by Raman microspectroscopy. Fertil Steril 98:1124\u20131129","journal-title":"Fertil Steril"},{"key":"1537_CR21","doi-asserted-by":"publisher","first-page":"e0207786","DOI":"10.1371\/journal.pone.0207786","volume":"13","author":"R Da Costa","year":"2018","unstructured":"Da Costa\u00a0R, Amaral\u00a0S, Redmann\u00a0K et al (2018) Spectral features of nuclear DNA in human sperm assessed by Raman Microspectroscopy: effects of UV-irradiation and hydration. PLoS ONE 13:e207786","journal-title":"PLoS ONE"},{"key":"1537_CR22","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1038\/nature09769","volume":"471","author":"T Str\u00fcnker","year":"2011","unstructured":"Str\u00fcnker\u00a0T, Goodwin\u00a0N, Brenker\u00a0C et al (2011) The CatSper channel mediates progesterone-induced Ca2+ influx in human sperm. Nature 471:382\u2013386","journal-title":"Nature"}],"container-title":["Der Urologe"],"original-title":[],"language":"de","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00120-021-01537-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00120-021-01537-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00120-021-01537-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,26]],"date-time":"2022-12-26T01:28:23Z","timestamp":1672018103000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00120-021-01537-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,30]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["1537"],"URL":"https:\/\/doi.org\/10.1007\/s00120-021-01537-1","relation":{},"ISSN":["0340-2592","1433-0563"],"issn-type":[{"value":"0340-2592","type":"print"},{"value":"1433-0563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,30]]},"assertion":[{"value":"14 April 2021","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 April 2021","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Einhaltung ethischer Richtlinien"}},{"value":"Gem\u00e4\u00df den Richtlinien des Springer Medizin Verlags werden Autoren und Wissenschaftliche Leitung im Rahmen der Manuskripterstellung und Manuskriptfreigabe aufgefordert, eine vollst\u00e4ndige Erkl\u00e4rung zu ihren finanziellen und nichtfinanziellen Interessen abzugeben.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Interessenkonflikt"}},{"value":"<b>S. Kliesch<\/b>: A. Finanzielle Interessen: S. Kliesch gibt an, dass kein finanzieller Interessenkonflikt besteht.\u00a0\u2013 B. Nichtfinanzielle Interessen: Chef\u00e4rztin der Abteilung f\u00fcr Klinische und Operative Andrologie, Centrum f\u00fcr Reproduktionsmedizin und Andrologie, Universit\u00e4tsklinikum M\u00fcnster | Vorsitzende der Fachgruppe D4 \u201eEjakulatdiagnostik\u201c der Bundes\u00e4rztekammer, Pr\u00e4sidentin der Deutschen Gesellschaft f\u00fcr Andrologie, Vorsitzende des Arbeitskreises Andrologie der Deutschen Gesellschaft f\u00fcr Urologie.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Autoren"}},{"value":"Die vollst\u00e4ndige Erkl\u00e4rung zum Interessenkonflikt der Wissenschaftlichen Leitung finden Sie am Kurs der zertifizierten Fortbildung auf.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Wissenschaftliche Leitung"}},{"value":"erkl\u00e4rt, dass f\u00fcr die Publikation dieser CME-Fortbildung keine Sponsorengelder an den Verlag flie\u00dfen.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Der Verlag"}},{"value":"F\u00fcr diesen Beitrag wurden von den Autoren keine Studien an Menschen oder Tieren durchgef\u00fchrt. F\u00fcr die aufgef\u00fchrten Studien gelten die jeweils dort angegebenen ethischen Richtlinien.","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Der Verlag"}}]}}