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However, the\u00a0natural Ogura CMS restorer line has not been found in<jats:italic>B. oleracea<\/jats:italic>crops. In this study, the triploid strategy was used with the aim to create euploid<jats:italic>B. oleracea<\/jats:italic>progenies with the<jats:italic>Rfo<\/jats:italic>gene. The allotriploid AAC hybrid YL2 was used as a male parent to backcross with Ogura CMS Chinese kale. After successive backcrosses, the BC<jats:sub>2<\/jats:sub><jats:italic>Rfo<\/jats:italic>-positive individual 16CMSF2-11 and its BC<jats:sub>3<\/jats:sub>progenies, with 18 chromosomes, were developed, which were morphologically identical to the parent Chinese kale. Compared with F<jats:sub>1<\/jats:sub>and BC<jats:sub>1<\/jats:sub>plants, it showed stable fertility performance, and regular meiosis behavior and could produce seeds normally under natural pollination. The genomic composition analysis of<jats:italic>Rfo<\/jats:italic>-positive progenies by using molecular markers showed that more than 87% of the C-genome components of BC<jats:sub>3<\/jats:sub><jats:italic>Rfo<\/jats:italic>-progenies recovered to the parent Chinese kale, while most or all of the<jats:italic>A<\/jats:italic><jats:sub><jats:italic>n<\/jats:italic><\/jats:sub>-genome segments were lost in 16CMSF2-11 and its progenies. The results suggested that the genetic background of<jats:italic>Rfo<\/jats:italic>-positive individuals was closer to that of the parent Chinese kale along with backcrossing. Hereof, the Ogura CMS fertility-restored materials of Chinese kale were successfully created via triploid strategy for the first time, providing a bridge for utilizing the Ogura CMS<jats:italic>B. oleracea<\/jats:italic>germplasm in the future. Moreover, our study indicates that the triploid strategy is effective for transferring genes from<jats:italic>B. napus<\/jats:italic>into<jats:italic>B. oleracea<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1007\/s00122-020-03635-8","type":"journal-article","created":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T10:39:48Z","timestamp":1593599988000},"page":"2825-2837","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Creation of fertility-restored materials for Ogura CMS in Brassica oleracea by introducing Rfo gene from Brassica napus via an allotriploid strategy"],"prefix":"10.1007","volume":"133","author":[{"given":"Hai-long","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-yuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-jing","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-qing","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-mei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mu","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-hao","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-mei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-yuan","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1337-4899","authenticated-orcid":false,"given":"Yang-yong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,1]]},"reference":[{"key":"3635_CR1","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1007\/BF00041511","volume":"36","author":"R Ayotte","year":"1987","unstructured":"Ayotte R, Harney PM, Machado VS (1987) The transfer of triazine resistance from Brassica napus L. to B. oleracea L. 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