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In contrast,\n                      <jats:italic>C. annuum<\/jats:italic>\n                      cv. Feherozon (CaFo) is susceptible to XeR6. Map-based cloning of the\n                      <jats:italic>bs6<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>61<\/jats:italic>\n                      <\/jats:sup>\n                      locus revealed that the\n                      <jats:italic>bs6<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>61<\/jats:italic>\n                      <\/jats:sup>\n                      locus encodes three predicted proteins, pbs6\n                      <jats:sup>61<\/jats:sup>\n                      npk, pbs6\n                      <jats:sup>61<\/jats:sup>\n                      n, pbs6\n                      <jats:sup>61<\/jats:sup>\n                      pk in Ca61R which are the counterpart of the three wild type predicted proteins, pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      NPK, pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      N and pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      PK in CaFo, respectively. Sequence analysis revealed that the pBs6FoNPK protein showed 35% identity and 53% similarity to the ZRK4 pseudokinase (pAtZRK4) identified in\n                      <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                      , which possesses N-terminal and C-terminal (pseudokinase) domains. The pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      N and pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      PK proteins resemble the N-terminal and C-terminal domains of the pBs6FoNPK protein, respectively, thus, they also contain pseudokinase domains\/sequences. In Ca61R, pbs6\n                      <jats:sup>61<\/jats:sup>\n                      npk and pbs6\n                      <jats:sup>61<\/jats:sup>\n                      n are truncated counterparts of pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      NPK and pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      N, respectively, as a consequence of a single-nucleotide insertion that causes frameshifts and premature stop codons, whereas pbs6\n                      <jats:sup>61<\/jats:sup>\n                      pk differs from pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      PK by a single amino acid, a serine to alanine substitution at position 133. These three proteins are considered functionless. The ORF of pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      N, pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      PK and pbs6\n                      <jats:sup>61<\/jats:sup>\n                      n, pbs6\n                      <jats:sup>61<\/jats:sup>\n                      pk, respectively, are encoded by a single transcript in both parental plants. For this reason the translation of pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      PK was ambiguous, however, the expression of pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      N-dsRed fusion protein demonstrated that pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      PK in fact can be translated. The three wild type proteins (pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      NPK, pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      N and pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      PK) were independently responsible for ETS (effector-triggered susceptibility), because all three genes had to be in mutant configuration to manifest the resistant phenotype, in addition, any one of the three was able to reverse the resistant phenotype to susceptibility. Functional analysis using virus-induced gene silencing and transient genetic complementation demonstrated that the three CaFo proteins contribute redundantly to XeR6 susceptibility. Silencing either Bs6\n                      <jats:sup>Fo<\/jats:sup>\n                      NPK, Bs6\n                      <jats:sup>Fo<\/jats:sup>\n                      N and Bs6\n                      <jats:sup>Fo<\/jats:sup>\n                      PK alone did not alter susceptibility, whereas simultaneous silencing of these genes conferred resistance. Conversely, the individual expression of pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      NPK, pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      N, and pBs6\n                      <jats:sup>Fo<\/jats:sup>\n                      PK, in the resistant Ca61R background each restored susceptibility. These results demonstrate that the three Bs6 proteins act redundantly to promote XeR6 susceptibility and that loss of function of all three corresponding proteins was required for the recessive resistance phenotype conferred by\n                      <jats:italic>bs6<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>61<\/jats:italic>\n                      <\/jats:sup>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00122-026-05407-2","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T09:56:00Z","timestamp":1792490160000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Map-based cloning of the susceptibility locus conditioning bs6 resistance in pepper (Capsicum annuum L.) encoding mutant alleles of pseudokinase proteins"],"prefix":"10.1007","volume":"139","author":[{"given":"Zoltan","family":"Toth","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoltan Gabor","family":"Toth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Kalo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyorgy B.","family":"Kiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2001-0375","authenticated-orcid":false,"given":"Zoltan","family":"Szabo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,20]]},"reference":[{"key":"5407_CR1","doi-asserted-by":"publisher","first-page":"1163","DOI":"10.1111\/mpp.12821","volume":"20","author":"P Balint-Kurti","year":"2019","unstructured":"Balint-Kurti P (2019) The plant hypersensitive response: concepts, control and consequences. 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